温度对太平洋冈比亚藻Gambierdiscus pacificus的生长和多糖产量的影响*

doi:10.11978/2015158

热带海洋学报 ›› 2016, Vol. 35 ›› Issue (5): 55-61.doi: 10.11978/2015158CSTR: 32234.14.2015158

温度对太平洋冈比亚藻Gambierdiscus pacificus的生长和多糖产量的影响^*

吴珍¹, 张华¹, 岑竞仪¹, 李丽¹, 晁爱敏², 吕颂辉¹

1. 暨南大学赤潮与海洋生物学研究中心, 广东广州 510632; 2. 浙江省环境监测中心生态监测与评价研究所, 浙江杭州 310015

收稿日期:2015-12-29 出版日期:2016-08-29 发布日期:2016-09-22
通讯作者: 吕颂辉。E-mail: lusonghui1963@163.com
作者简介:吴珍(1991—), 女, 湖北省武汉市人, 硕士, 从事海洋藻类生理生态学研究。E-mail: wwonka@126.com
基金资助:
基金项目：国家自然科学基金(31372535、41576162); 广东省科技计划项目(2016A030303012); 广东省海洋推广专项(A201501D08)

Effects of temperature on the growth and carbohydrate production of Gambierdiscus pacificus

WU Zhen¹, ZHANG Hua¹, CEN Jingyi¹, LI Li¹, CHAO Aimin², LÜ Songhui¹

1. Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, China;; 2. Ecological Monitoring & Appraisal Ins., Zhejiang Environmental Monitoring Center, Hangzhou 310015, China

Received:2015-12-29 Online:2016-08-29 Published:2016-09-22
Contact: Corresponding author: LÜ Songhui. E-mail: lusonghui1963@163.com
Supported by:
Foundation item: National Nature Science Foundation (31372535, 41576162); Guangdong Provincial Science and Technology Project (2016A030303012); Guangdong Provincial Oceanic Special Promotion (A201501D08)

摘要/Abstract

摘要： 实验检测了一株分离于中国海南岛海域的太平洋冈比亚藻Gambierdiscus pacificus在5种温度下的生长状况、光合活性以及多糖产量。太平洋冈比亚藻的生长温度耐受范围在20~35℃之间, 15℃时细胞没有生长并在较短时间内死亡, 25℃时达到最大生长速率, 为0.40d^-1, 30℃时细胞密度最大, 达到5625个•mL^-1。在不同温度下太平洋冈比亚藻的生长周期不同, 25℃、30℃和35℃时生长周期分别是34、34和25天, 而在20℃时生长周期长达55天。该藻在25℃时光合活性最强, 最大光化学量子产量(F_v/F_m)值达到0.75, 而在其生长受限温度15℃时连续下降。通过测定太平洋冈比亚藻在其各耐受温度之下的稳定期和衰亡期产生的粘连多糖和非粘连多糖含量, 发现20℃和35℃时单位细胞的多糖产量要高于25℃和30℃, 衰亡期一般高于稳定期, 各温度下同一时期细胞产生的非粘连多糖含量高于粘连多糖含量。温度是一个重要生态因子, 通过研究太平洋冈比亚藻在不同温度下的生长、光合活性以及多糖产量对了解它的生长特性并获知其大致分布范围至关重要。

关键词: 太平洋冈比亚藻, 生长速率, 生物量, 光合活性, 多糖产量

Abstract: Our study mainly focused on the growth photosynthetic characteristics and carbohydrate production of a strain of Gambierdiscus pacificus from Hainan Island under five temperature settings. G. pacificus could survive under temperatures from 20℃ to 35℃. At 15℃, G. pacificus cells could not survive and would die in a short time. The specific growth rate was found to be highest at 25℃ (0.40 d^-1) and the largest cell concentration occurred at 30℃ (5625 cells•mL^-1). G. pacificus had different lengths of life cycle under different temperatures. It could survive for 34, 34 and 25 days at 25℃, 30℃ and 35℃, respectively, while the length of its life cycle at 20℃ could be as long as 55 days. The highest F_v/F_m (maximal quantum yield of PSII) value of G. pacificus was 0.75, which was found at 25℃, but the value kept declining at its limiting temperature of 15℃. Through measuring the carbohydrate yield of G. pacificus at its tolerable temperatures, we found that the cell carbohydrate production of G. pacificus was the highest at the temperature range that was suboptimal for cell growth. The non-attached carbohydrate yield were always higher than attached carbohydrate yield in every temperature and growth phase in our study and carbohydrate concentration was statistically higher during the decaying phase compared to that of the stationary phase. Since temperature is an important ecological factor, studying temperature requirements of G. pacificus is of great significance to grasp its growth characteristics and predict the scope of its general distribution.

Key words: Gambierdiscus pacificus, growth rate, biomass, photosynthesis characteristics, carbohydrate production

吴珍, 张华, 岑竞仪, 李丽, 晁爱敏, 吕颂辉. 温度对太平洋冈比亚藻Gambierdiscus pacificus的生长和多糖产量的影响^*[J]. 热带海洋学报, 2016, 35(5): 55-61.

WU Zhen, ZHANG Hua, CEN Jingyi, LI Li, CHAO Aimin, LÜ Songhui. Effects of temperature on the growth and carbohydrate production of Gambierdiscus pacificus[J]. Journal of Tropical Oceanography, 2016, 35(5): 55-61.

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温度对太平洋冈比亚藻Gambierdiscus pacificus的生长和多糖产量的影响^*

Effects of temperature on the growth and carbohydrate production of Gambierdiscus pacificus

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