中国沿海尖刺拟菱形藻的种下分类学研究*

doi:10.11978/2017017

热带海洋学报 ›› 2018, Vol. 37 ›› Issue (1): 12-19.doi: 10.11978/2017017CSTR: 32234.14.2017017

中国沿海尖刺拟菱形藻的种下分类学研究^*

董焕嫦^1,²(), 黄春秀^1,², 徐国双^1,², 李扬^1,²()

1. 华南师范大学生命科学学院, 广州市亚热带生物多样性与环境生物监测重点实验室, 广州广东 510631
2. 华南师范大学生命科学学院, 广东省水产健康安全养殖重点实验室, 广州广东 510631

收稿日期:2017-02-15 修回日期:2017-05-26 出版日期:2018-01-20 发布日期:2018-02-02
作者简介:
作者简介：董焕嫦(1993—), 女, 汉族, 广东省肇庆市人, 硕士研究生, 主要从事藻类学研究。E-mail: 460324827@qq.com
基金资助:
国家自然科学基金(31570205、31370235);广州市科技计划项目(201607010370)

Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters^*

Huanchang DONG^1,²(), Chunxiu HUANG^1,², Guoshuang XU^1,², Yang LI^1,²()

1. Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, College of Life Science, South China Normal University, Guangzhou 510631, China
2. Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture, College of Life Science, South China Normal University,Guangzhou 510631, China

Received:2017-02-15 Revised:2017-05-26 Online:2018-01-20 Published:2018-02-02
Supported by:
National Natural Science Foundation of China (31570205, 31370235);Science and Technology Planning Project of Guangzhou, China (201607010370)

摘要/Abstract

摘要：

为了澄清中国沿海尖刺拟菱形藻Pseudo-nitzschia pungens Hasle的种下分类学现状, 从我国沿海分离并建立了32个尖刺拟菱形藻的单克隆培养株系, 结合光学显微镜下的群体特征和透射电镜下的超微形态学特征, 以及基于核糖体转录间隔区(ITS)的分子系统学数据, 鉴定得到尖刺拟菱形藻的两个变种: 尖刺拟菱形藻原变种P. pungens var. pungens和尖刺拟菱形藻阿维罗变种P. pungens var. averiensis。文章对其形态学特征进行了详细描述, 并利用高效液相色谱-质谱联用法(LC-MS/MS)对培养株系的多莫酸(DA)进行了检测, 但未检测到DA。

关键词: 尖刺拟菱形藻, 种下分类学, 形态, 核糖体转录间隔区, 多莫酸

Abstract:

To declare intraspecies diversity of Pseudo-nitzschia pungens Hasle, a total of 32 monoclonal strains of P. pungens from Chinese coastal waters were isolated and established. These strains were identified carefully, based on the morphology under both light microscopy and transmission electron microscopy, and on the molecular analysis inferred from the internal transcribed spacer region sequences. Two varieties were identified: P. pungens var. pungens (Grunow) Hasle and P. pungens var. averiensis Lundholm, Churro, Carreira, and Calado. Their morphologies were described and compared with allied taxa. Meanwhile, domoic acid (DA) was detected using a liquid chromatography tandem mass spectrometry (LC-MS/MS) injection, but no detectable DA was found. The results may provide detailed information for further studies of toxin production of Chinese Pseudo-nitzschia strains.

Key words: Pseudo-nitzschia pungens, taxonomy, morphology, internal transcribed spacer, domoic acid

中图分类号:

P735.531

董焕嫦, 黄春秀, 徐国双, 李扬. 中国沿海尖刺拟菱形藻的种下分类学研究^*[J]. 热带海洋学报, 2018, 37(1): 12-19.

Huanchang DONG, Chunxiu HUANG, Guoshuang XU, Yang LI. Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters^*[J]. Journal of Tropical Oceanography, 2018, 37(1): 12-19.

图/表 4

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株系	分离地点	采样时间	种名
MC215	珠海	2013年5月	P. pungens var. pungens
MC218	葫芦岛	2013年8月	P. pungens var. pungens
MC241	惠州	2013年10月	P. pungens var. averiensis
MC255	厦门	2013年11月	P. pungens var. averiensis
MC263	青岛	2013年11月	P. pungens var. averiensis
MC289	青岛	2014年2月	P. pungens var. pungens
MC290	青岛	2014年2月	P. pungens var. pungens
MC295	惠州	2014年4月	P. pungens var. pungens
MC404	惠州	2014年7月	P. pungens var. averiensis
MC830	温州	2015年5月	P. pungens var. pungens
MC831	温州	2015年5月	P. pungens var. pungens
MC841	香港	2015年7月	P. pungens var. averiensis
MC864	宁波	2015年8月	P. pungens var. averiensis
MC867	宁波	2015年8月	P. pungens var. averiensis
MC881	湛江	2015年8月	P. pungens var. averiensis
MC888	湛江	2015年8月	P. pungens var. averiensis
MC899	青岛	2015年9月	P. pungens var. averiensis
MC915	深圳	2015年9月	P. pungens var. averiensis
MC988	台湾海峡	2016年5月	P. pungens var. pungens
MC3052	珠海	2016年5月	P. pungens var. pungens
MC3053	珠海	2016年5月	P. pungens var. averiensis
MC3054	珠海	2016年5月	P. pungens var. pungens
MC3057	珠海	2016年5月	P. pungens var. pungens
MC3058	珠海	2016年5月	P. pungens var. pungens
MC3059	珠海	2016年5月	P. pungens var. pungens
MC3060	珠海	2016年5月	P. pungens var. averiensis
MC3061	珠海	2016年5月	P. pungens var. pungens
MC3062	珠海	2016年5月	P. pungens var. pungens
MC3065	珠海	2016年5月	P. pungens var. pungens
MC3067	珠海	2016年5月	P. pungens var. pungens
MC3119	黄海 N:122°50′, E:32°00′	2016年7月	P. pungens var. pungens
MC3195	台湾海峡	2016年8月	P. pungens var. averiensis

中国沿海尖刺拟菱形藻的种下分类学研究^*

Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters^*

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中国沿海尖刺拟菱形藻的种下分类学研究*

Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters*

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中国沿海尖刺拟菱形藻的种下分类学研究^*

Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters^*