广西涠洲岛底栖甲藻前沟藻属(Amphidinium)种类的形态学和系统发育学研究

doi:10.11978/2019077

摘要/Abstract

摘要：

本文对采集自我国广西涠洲岛海域潮下带大型海藻及死珊瑚上的附着底栖甲藻样品进行了分析。利用毛细管单细胞分离方法成功分离并培养了多株前沟藻属种类, 运用光学显微镜、扫描电镜和分子生物学等技术, 对所获得的前沟藻株系进行形态学和系统发育分析, 鉴定出前沟藻属3个种, 分别为具盖前沟藻(Amphidinium operculatum)、玛氏前沟藻(Amphidinium massertii)和强壮前沟藻(Amphidinium caeterae), 其中具盖前沟藻和玛氏前沟藻为我国新纪录种。分析比较了不同种类间形态和系统发育的差异, 发现3种前沟藻的形态特征大体与相应模式种报道的一致。本研究丰富了我国前沟藻种类多样性和系统发育进化信息, 同时将两个新记录种具盖前沟藻和玛氏前沟藻的已知分布范围拓展到了我国南部沿海水域。

关键词: 涠洲岛, 前沟藻, 形态学, 系统发育

Abstract:

In this study, strains of Amphidinium from seaweeds and dead corals around Weizhou Island, Guangxi, China were isolated by single cell capillary pipette and cultured. The morphological features and phylogenetic analysis were determined based on light microscopy, scanning electron microscopy and molecular biology techiniques. Three Amphidinium species were identified, namely, Amphidinium operculatum, Amphidinium massertii and Amphidinium caeterae, among which A. operculatum and A. massertii were new records in China. The differences in morphology and phylogeny between different species were analyzed and compared. It was found that the morphological characteristics of the three Amphidinium species were significantly consistent with model species. This study adds information to the diversity and phylogenetic evolution of Amphidinium species in China, and extented the distribution of two new Amphidinium species to the coastal waters of southern China.

Key words: Weizhou Island, Amphidinium, morphology, phylogeny

江丽春, 李群, 吕颂辉. 广西涠洲岛底栖甲藻前沟藻属(Amphidinium)种类的形态学和系统发育学研究[J]. 热带海洋学报, 2020, 39(3): 106-115.

Lichun JIANG, Qun LI, Songhui LÜ. Morphology and Phylogenetics study on the species of Amphidinium (Gymnodinials, Dinophyceae) from Weizhou Island, Guangxi[J]. Journal of Tropical Oceanography, 2020, 39(3): 106-115.

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种名	长/μm	宽/μm	形状	纵鞭毛插入位置	蛋白核	泡	体鳞
具盖前沟藻(A.Operculatum)	33~48	22~37	卵圆形	偏细胞底端1/3处	无	2个, 位于两个鞭毛孔处	无
玛氏前沟藻(A.Massartii)	10~16	7~12	卵圆形或椭圆形	细胞中部	1个, 细胞中部	无	有
强壮前沟藻(A.Carterae)	12~19	9~14	卵圆形	细胞中部	1个, 细胞中部	无	无

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