三亚海域浮游病毒与细菌丰度的时空变化规律以及相关环境因子*

doi:10.11978/2017064

热带海洋学报 ›› 2018, Vol. 37 ›› Issue (2): 36-46.doi: 10.11978/2017064CSTR: 32234.14.2017064

三亚海域浮游病毒与细菌丰度的时空变化规律以及相关环境因子^*

荆红梅¹(), 韦誉^1,², 郑丽平¹, 许秋彤^1,², 张玥^1,², 朱文达^1,²

1. 中科院深海极端环境模拟重点实验室, 中国科学院深海科学与工程研究所, 海南三亚 572000
2. 中国科学院大学, 北京 100049

收稿日期:2017-05-26 修回日期:2017-09-27 出版日期:2018-04-10 发布日期:2018-04-11
作者简介:
作者简介：荆红梅(1977—), 女, 河南省新乡市人, 主要从事海洋微生物分子生态学研究。Email: hmjing@idsse.ac.cn
基金资助:
中国科学院知识创新工程项目(SIDSSE-201303);国家自然科学基金(41406180)

Spatiotemporal variations of virioplankton and bacterioplankton abundance in Sanya waters and their related environmental factors

Hongmei JING¹(), Yu WEI^1,², Liping ZHENG¹, Qiutong XU^1,², Yue ZHANG^1,², Wenda ZHU^1,²

1. Chinese Academy of Sciences Key Lab for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2017-05-26 Revised:2017-09-27 Online:2018-04-10 Published:2018-04-11
Supported by:
Knowledge Innovation Program of the Chinese Academy of Sciences (SIDSSE-201303);The National Natural Science Foundation of China (41406180)

摘要/Abstract

摘要：

在三亚海域设置3个站位, 分别于各站位连续采集12个月表层水样。利用流式细胞仪进行浮游病毒及浮游细菌丰度的测定, 并对两者及其与环境因子之间的相关性进行研究, 同时对不同站位之间进行差异性分析。结果表明, 调查海域浮游病毒丰度(平均7.63×10⁶viruses·mL^-1)高于浮游细菌丰度(平均1.52×10⁶cells·mL^-1)。浮游病毒及浮游细菌丰度在三亚河口最高, 且有明显的季节变化, 冬春季高于夏秋季, 并且其与鹿回头半岛西侧、小东海之间均有极显著差异(P<0.01), 不同类群病毒代表的宿主类群也有所不同。调查海域总体水平浮游病毒丰度与浮游细菌丰度显著正相关(r=0.800, P<0.01); 叶绿素(Chl a)和氮盐(NO- 2、NO- 2+NO- 3、NH+ 4)是影响两者的关键因子。

关键词: 三亚, 浮游病毒, 浮游细菌, 流式细胞仪

Abstract:

Surface water samples were collected monthly for a total of 12 months from three stations in Sanya. Their virioplankton and bacterioplankton abundances were measured by flow cytometry and their correlationships with evnironmental factors were also studied. The results show that the abundance of virioplankton (7.63×10⁶viruses·mL^-1on average) was higher than bacterioplankton abundance (1.52×10⁶cells·mL^-1on average). Virioplankton and bacterioplankton abundances in the Estuary of Sanya River were significantly different from those on the west side of the Luhuitou Peninsula and Xiaodonghai. The highest virioplankton and bacterioplankton abundances appeared in the Estuary of Sanya River, with obvious seasonal variation(P<0.01). The abundances of virioplankton and bacterioplankton were significantly positively correlated (r = 0.800, P<0.01); and chlorophyll a, N nutrients (NO- 2, NO- 2+NO- 3, NH+ 4) are their key ecological factors (P < 0.01).

Key words: Sanya, virioplankton, bacterioplankton, flow cytometry

荆红梅, 韦誉, 郑丽平, 许秋彤, 张玥, 朱文达. 三亚海域浮游病毒与细菌丰度的时空变化规律以及相关环境因子^*[J]. 热带海洋学报, 2018, 37(2): 36-46.

Hongmei JING, Yu WEI, Liping ZHENG, Qiutong XU, Yue ZHANG, Wenda ZHU. Spatiotemporal variations of virioplankton and bacterioplankton abundance in Sanya waters and their related environmental factors[J]. Journal of Tropical Oceanography, 2018, 37(2): 36-46.

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站位	细菌		病毒V-Ⅰ		病毒V-Ⅱ		总病毒
站位	鹿回头半岛西侧	小东海	鹿回头半岛西侧	小东海	鹿回头半岛西侧	小东海	鹿回头半岛西侧	小东海
小东海	0.73		0.812		0.469		0.492
三亚河口	0.009^**	0.008^**	0.000**	0.000**	0.000**	0.000**	0.000**	0.000**

相关影响因子	Vir	Bac	VBR	Sal	Tem	pH	Chl a (>20μm)	Chl a (2~20μm)	Chl a (GF/F)	NO- 2	NO- 2 +NO- 3	PO3- 4	SiO2- 3
Bac	-0.034
VBR	0.632*	-0.549
Sal	-0.310	-0.337	-0.068
Tem	0.039	0.668*	-0.396	-0.826**
pH	0.395	0.287	-0.267	-0.221	0.371
Chl a (>20μm)	0.637*	-0.192	0.163	0.267	-0.335	0.507
Chl a (2~20μm)	-0.052	-0.424	0.060	0.724**	-0.769**	-0.134	0.571
Chl a (GF/F)	0.636*	-0.224	0.173	0.069	-0.124	0.669*	0.891**	0.387
NO- 2	-0.031	0.838**	-0.333	-0.185	0.397	0.087	-0.184	-0.194	-0.188
NO- 2+NO- 3	0.154	0.799**	-0.288	-0.281	0.432	0.188	0.022	-0.157	0.011	0.957**
PO3- 4	0.163	-0.410	0.431	0.155	-0.509	-0.036	0.265	0.545	0.204	-0.214	-0.206
SiO2- 3	0.049	0.686*	-0.230	-0.070	0.191	0.040	-0.001	-0.013	-0.057	0.942**	0.949**	-0.062
NH+ 4	0.005	0.777**	-0.238	-0.203	0.432	0.073	-0.235	-0.258	-0.147	0.955**	0.893**	-0.311	0.857**

相关影响因子	Vir	Bac	VBR	Sal	Tem	pH	Chl a (>20μm)	Chl a (2~20μm)	Chl a (GF/F)	NO- 2	NO- 2+NO- 3	PO3- 4	SiO2- 3
Bac	0.332
VBR	0.446	-0.636*
Sal	-0.019	-0.378	0.327
Tem	0.046	0.610*	-0.446	-0.791**
pH	-0.042	0.138	-0.210	0.064	0.369
Chl a(>20μm)	0.424	-0.408	0.553	0.393	-0.589*	-0.183
Chl a(2~20μm)	-0.114	-0.412	0.315	0.027	0.068	0.514	0.002
Chl a(GF/F)	0.278	-0.422	0.438	-0.015	-0.146	0.318	0.605*	0.601*
NO- 2	-0.491	0.046	-0.499	-0.486	0.348	0.255	-0.433	-0.103	-0.112
NO- 2+NO- 3	-0.247	0.241	-0.525	-0.541	0.450	0.023	-0.488	-0.241	-0.311	0.613*
PO3- 4	-0.285	0.148	-0.311	0.406	-0.193	-0.083	0.157	-0.364	-0.347	-0.18	-0.318
SiO2- 3	-0.290	0.036	-0.328	0.065	-0.240	-0.156	-0.305	-0.121	-0.254	0.353	0.334	-0.164
NH+ 4	0.638*	0.492	0.123	-0.074	0.253	0.281	-0.116	-0.052	0.057	-0.079	-0.303	-0.188	-0.200

相关影响因子	Vir	Bac	VBR	Sal	Tem	pH	Chl a (>20μm)	Chl a (2~20μm)	Chl a (GF/F)	NO- 2	NO- 2+NO- 3	PO3- 4	SiO2- 3
Bac	0.677*
VBR	0.164	-0.539
Sal	0.474	0.593*	-0.127
Tem	-0.563	-0.686*	0.205	-0.680*
pH	0.059	-0.409	0.720**	-0.140	0.433
Chl a(>20μm)	0.469	0.724**	-0.257	0.638*	-0.597*	-0.138
Chl a(2~20μm)	0.406	0.539	-0.230	0.367	-0.549	-0.219	0.089
Chl a(GF/F)	0.456	0.564	-0.167	0.394	-0.316	0.047	0.312	0.759**
NO- 2	0.531	0.452	0.159	0.351	-0.719**	-0.143	0.220	0.633*	0.250
NO- 2+NO- 3	0.109	0.078	0.153	-0.132	-0.517	-0.196	0.001	0.266	-0.171	0.776**
PO3- 4	0.403	-0.018	0.528	-0.002	-0.327	0.233	0.091	0.298	0.130	0.488	0.449
SiO2- 3	-0.033	-0.104	0.183	-0.257	-0.359	-0.137	-0.193	0.088	-0.351	0.600*	0.930**	0.397
NH+ 4	0.277	0.167	0.203	-0.016	-0.521	-0.074	-0.033	0.604*	0.181	0.851**	0.837**	0.712**	0.738**

相关影响因子	Vir	Bac	VBR	Sal	Tem	pH	Chl a (>20μm)	Chl a (2~20μm)	Chl a (GF/F)	NO- 2	NO- 2+NO- 3	PO3- 4	SiO2- 3
Bac	0.800**
VBR	0.331*	-0.230
Tem	-0.211	-0.218	-0.054	-0.621**
pH	-0.117	-0.273	0.236	-0.014	0.389*
Chl a(>20μm)	0.546**	0.614**	0.033	0.280	-0.461**	-0.042
Chl a(2~20μm)	0.582**	0.639**	-0.029	0.071	-0.303	-0.178	0.251
Chl a(GF/F)	0.689**	0.683**	0.087	-0.002	-0.180	-0.006	0.503**	0.804**
NO- 2	0.792**	0.697**	0.275	-0.147	-0.235	-0.187	0.379*	0.700**	0.556**
NO- 2+NO- 3	0.587**	0.497**	0.225	-0.405*	-0.086	-0.173	0.254	0.462**	0.315	0.887**
PO3- 4	0.134	-0.010	0.244	0.137	-0.250	0.049	0.088	0.133	0.030	0.143	0.086
SiO2- 3	0.359*	0.271	0.198	-0.353*	-0.142	-0.161	0.072	0.275	0.067	0.720**	0.908**	0.078
NH+ 4	0.714**	0.571**	0.312	-0.335*	-0.146	-0.162	0.271	0.677**	0.536**	0.938**	0.905**	0.204	0.771**

三亚海域浮游病毒与细菌丰度的时空变化规律以及相关环境因子^*

Spatiotemporal variations of virioplankton and bacterioplankton abundance in Sanya waters and their related environmental factors

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研究	细菌丰度/(×10⁸cells·L^-1)	时间	站位
周伟华等(2006)	11.32±7.26	2004秋	三亚湾
周伟华等(2007)	6.70±2.49	2005春	三亚湾
	8.41±2.51	2005夏	三亚湾
	5.78±2.81	2005秋	三亚湾
	8.84±4.67	2005冬	三亚湾
本研究	6.56±5.00	2014—2015	鹿回头西侧
	5.85±4.93	2014—2015	小东海
	33.3±29.19	2014—2015	三亚河口

[1]	胡思敏, 周天成, 张琛, 刘胜, 李涛, 黄晖. 悬浮物对三亚珊瑚礁区浮游动物群落结构及其摄食的影响[J]. 热带海洋学报, 2024, 43(3): 122-130.
[2]	梁宇娴, 刘骋跃, 俞晓磊, 张浴阳, 练文科, 陈伦举, 黄晖. 聚焦退化珊瑚礁关键造礁石珊瑚类群的补充与恢复—以三亚西岛为例[J]. 热带海洋学报, 2024, 43(3): 166-176.
[3]	张浴阳, 刘骋跃, 俞晓磊, 罗勇, 周天成, 练健生, 黄晖. 三亚凤凰岛造礁石珊瑚迁移效果研究^*[J]. 热带海洋学报, 2024, 43(3): 177-186.
[4]	李彩, 刘聪, 张现清, 陈飞, 肖志会, 杨泽明, 郑媛宁, 周雯, 许占堂. 体散射函数及衰减系数快速剖面仪的研发及应用^*[J]. 热带海洋学报, 2024, 43(2): 1-11.
[5]	雷新明, 黄晖, 练健生, 张浴阳, 杨剑辉. [已撤稿] 三亚珊瑚礁珊瑚藻种类及其空间特征与环境因子的关系[J]. 热带海洋学报, 2019, 38(3): 79-88.
[6]	王有军, 徐翠莲, 胡思敏, 李涛, 刘胜. 三亚湾近岸浮游纤毛虫群落的昼夜变化特征^*[J]. 热带海洋学报, 2017, 36(1): 72-80.
[7]	陈标, 陈永强, 黄晖, 谢强, 江玉凤, 雷新明, 江雷, 张诗泽, 周国伟. 南海三亚湾鹿回头海域风信子鹿角珊瑚反射率分析[J]. 热带海洋学报, 2015, 34(1): 71-76.
[8]	周凯, 章洁香, 张瑜斌, 卢东伟, 丁玉静, 孙省利. 深圳湾浮游细菌生物量的时空分布及其影响因素[J]. 热带海洋学报, 2013, 32(3): 65-71.
[9]	赵美霞,余克服,张乔民,施祺. 应用海洋生态足迹评价三亚珊瑚礁资源的可持续利用[J]. 热带海洋学报, 2011, 30(2): 74-80.
[10]	姜发军,胡章立,胡超群. 大鹏湾浮游细菌时空分布与环境因子的关系[J]. 热带海洋学报, 2011, 30(1): 96-100.