3种传统方法对不同珊瑚表面积测量的适用性及其校准方法——以3D扫描技术为基准*

doi:10.11978/2019039

热带海洋学报 ›› 2020, Vol. 39 ›› Issue (1): 85-93.doi: 10.11978/2019039CSTR: 32234.14.2019039

3种传统方法对不同珊瑚表面积测量的适用性及其校准方法——以3D扫描技术为基准^*

梁宇娴^1,^2,^3,^5,⁶, 俞晓磊^1,^2,^3,^5,⁶, 郭亚娟⁴, 黄晖^1,^2,^3,⁶, 周伟华^1,^2,^3,⁶(), 袁翔城^1,^2,⁶()

1. 中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室, 广东广州 510301
2. 中国科学院南海海洋研究所广东省应用海洋生物学重点实验室, 广东广州 510301
3. 中国科学院海南热带海洋生物实验站, 海南三亚 572000
4. 中国科学院南海海洋研究所海洋环境检测中心, 广东广州 510301
5. 中国科学院大学, 北京 100049
6. 海南省海洋生物技术重点实验室, 海南三亚 572000

收稿日期:2019-04-19 修回日期:2019-06-19 出版日期:2020-01-20 发布日期:2020-01-09
通讯作者: 周伟华,袁翔城
作者简介:梁宇娴(1992—), 女, 广东省湛江市人, 硕士, 从事珊瑚生物学和珊瑚礁生态学研究。E-mail: liangyuxian17@mails.ucas.ac.cn
基金资助:
中国科学院战略性先导科技专项(XDA13020403);国家重点研发计划(2017YFC0506301);国家自然科学基金项目(31370500)

Applicability and calibration methods of three traditional surface area measurement methods for different coral species — based on 3D scanning technology

LIANG Yuxian^1,^2,^3,^5,⁶, YU Xiaolei^1,^2,^3,^5,⁶, GUO Yajuan⁴, HUANG Hui^1,^2,^3,⁶, ZHOU Weihua^1,^2,^3,⁶(), YUAN Xiangcheng^1,^2,⁶()

1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
3. Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya 572000, China
4. Testing Center of Marine Environment, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
5. University of Chinese Academy of Sciences, Beijing 100049, China
6. Hainan Key Laboratory of Marine Biotechnology, Sanya 572000, China

Received:2019-04-19 Revised:2019-06-19 Online:2020-01-20 Published:2020-01-09
Contact: Weihua ZHOU,Xiangcheng YUAN
Supported by:
Strategic Priority Research Program of the Chinese Academy of Sciences(XDA13020403);National Key Research and Development Program of China(2017YFC0506301);National Natural Science Foundation of China(31370500)

摘要/Abstract

摘要：

造礁石珊瑚的表面积是珊瑚生物学和珊瑚礁生态学研究中重要的参数, 因其形态结构复杂, 准确测量十分困难。由于测量方法的不同, 导致不同珊瑚之间的重要指标无法被直接比较, 因此系统比较不同的表面积测量方法十分必要。以块状的普哥滨珊瑚Porites pukoensis和分枝状的鹿角杯形珊瑚Pocillopora damicornis为研究对象, 以3D扫描技术测量的珊瑚表面积作为标准值, 对比石蜡包埋法、锡箔纸包裹法以及简单几何近似法3种传统方法测量珊瑚表面积的准确度; 讨论了不同的表面积测量方法对于不同形态结构珊瑚的适用性; 并通过简单回归分析, 校准传统测量方法的测量准确度。研究结果表明, 无论在普哥滨珊瑚还是鹿角杯形珊瑚中, 简单几何近似法的测量准确度最高(72.71%和94.52%), 石蜡包埋法其次(68.86%和83.08%), 锡箔纸包裹法最低(65.27%和58.07%)。简单回归分析结果表明, 除了锡箔纸包裹法测量普哥滨珊瑚表面积与3D扫描测量结果的相关性较低(r²=0.76)外, 其他传统测量方法与3D扫描测量结果均具有较强相关性(r²>0.95)。因此, 研究认为可通过简单回归分析构建回归方程对传统测量方法进行校准, 以提高传统方法的测量准确度。

关键词: 造礁石珊瑚, 表面积测量方法, 3D扫描技术, 简单回归分析

Abstract:

The surface area of scleractinian corals is an important reference parameter required for various researchs of coral biology and coral reef ecology. However, due to its complex morphological structure, the accurate measurement of coral surface area remains difficult. The diversity of measurement methods leads to the unreachable direct comparison of important indicators between different coral species. Thus, it is necessary to make a systematic comparison of different surface area measurement methods in different coral species. Regarding coral surface area measured by 3D scanning technique as standard, this study investigated the accuracy of three traditional measurement methods (wax coating, aluminium foil and simple geometry), discussed the applicability of different methods for corals in different morphology, and calibrated the accuracy of traditional measurement methods by simple regression analysis of two scleractinian corals Porites pukoensis (massive) and Pocillopora damicornis (branching). Results showed that the accuracy of simple geometry method was the highest (72.71% and 94.52%), followed by that of wax coating method (68.86% and 83.08%), and the accuracy of aluminium foil method is the lowest (65.27% and 58.07%) for P. pukoensis and P. damicornis, respectively. A strong correlation between 3D scanning method and each of the three traditional measurement methods (r²>0.95) was revealed by simple regression analysis, except for the surface area of P. pukoensis measured by the aluminium foil method (r²=0.762). Overall, to improve the accuracy of traditional coral surface area measurement methods, regression equations constructed by simple regression analysis can be used for calibration.

Key words: scleractinian coral, surface area measurement method, 3D scanning technique, simple regression analysis

中图分类号:

P735

梁宇娴, 俞晓磊, 郭亚娟, 黄晖, 周伟华, 袁翔城. 3种传统方法对不同珊瑚表面积测量的适用性及其校准方法——以3D扫描技术为基准^*[J]. 热带海洋学报, 2020, 39(1): 85-93.

LIANG Yuxian, YU Xiaolei, GUO Yajuan, HUANG Hui, ZHOU Weihua, YUAN Xiangcheng. Applicability and calibration methods of three traditional surface area measurement methods for different coral species — based on 3D scanning technology[J]. Journal of Tropical Oceanography, 2020, 39(1): 85-93.

图/表 8

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几何体	表面积公式
圆台	S=π(r2+Rl+rl)
圆柱	S=πr(r+2h)
三角形	S=ah/2
半球	S=2πr2
梯形	S=(a₁+a₂)h/2

样品编号	3D扫描方法		石蜡包埋法		锡箔纸包裹法		简单几何近似法
样品编号	表面积/cm²	SD	表面积/cm²	SD	表面积/cm²	SD	表面积/cm²	SD
A1	37.59	0.84	38.68	6.43	55.42	2.73	37.07	0.49
A2	14.13	0.49	19.06	1.45	28.30	3.81	14.22	0.70
A3	11.46	0.08	14.83	2.57	20.74	3.96	11.26	0.78
A4	31.20	0.06	31.70	2.84	46.57	7.82	30.66	0.47
A5	8.95	0.09	13.49	1.28	18.28	3.54	8.65	0.37
B1	20.82	0.15	27.92	2.85	34.76	2.99	23.90	1.22
B2	19.94	0.09	36.46	3.56	37.09	3.67	14.69	0.74
B3	22.66	0.34	30.44	0.95	32.22	1.05	19.57	1.12
B4	14.46	0.29	20.66	1.18	19.45	1.83	10.28	0.25
B5	15.70	0.24	22.25	1.98	11.90	0.82	11.95	0.26

方法	每个样本的测量时间
方法	鹿角杯形珊瑚/min	普哥滨珊瑚/min
3D扫描方法	10~50	15~60
石蜡包埋法	15±2	15±2
锡箔纸包裹法	7~20	3~5
简单几何近似法	6~30	3~10

方法	原位测量	珊瑚整体水平测量	珊瑚礁群落水平测量	准确性	参考文献
投影面积	适用	适用	适用	低	Odum et al, 1955
锡箔纸包裹法	不适用	受限制	不适用	较高	Marsh et al, 1970
平面比例估算方法	适用	适用	适用	低	Dahl, 1973; Chancerelle, 2000, Courtney et al, 2007
乳胶包埋法	不适用	受限制	不适用	较高	Meyer et al, 1985
扫描法	不适用	受限制	不适用	高	Kaandorp et al, 2001
染料包埋法	不适用	适用	不适用	较高	Hoegh-Guldberg, 1988
石蜡包埋法	不适用	受限制	不适用	较高	Stimson et al, 1991
摄影测量技术	适用	受限制	不适用	受时间限制	Bythell et al, 2001
3D重构技术	适用	受限制	不适用	受时间限制	Cocito et al, 2003

3种传统方法对不同珊瑚表面积测量的适用性及其校准方法——以3D扫描技术为基准^*

Applicability and calibration methods of three traditional surface area measurement methods for different coral species — based on 3D scanning technology

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方法	鹿角杯形珊瑚	普哥滨珊瑚
石蜡包埋法	83.08±11.28	68.86±8.19
锡箔纸包裹法	58.07±6.70	65.27±8.32
简单几何近似法	94.52±7.31	72.71±12.30

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