低盐胁迫下假微型海链藻脂肪酸代谢的转录组学分析

doi:10.11978/2022250

热带海洋学报 ›› 2023, Vol. 42 ›› Issue (5): 92-103.doi: 10.11978/2022250CSTR: 32234.14.2022250

低盐胁迫下假微型海链藻脂肪酸代谢的转录组学分析

孙文杰¹^,²(), 李佳岷¹^,², 王华龙³, 米铁柱¹^,²^,⁴(), 甄毓¹^,²^,⁴

1. 中国海洋大学环境科学与工程学院, 山东青岛 266100
2. 中国海洋大学海洋环境与生态教育部重点实验室, 山东青岛 266100
3. 中国海洋大学海洋生命学院, 山东青岛 266003
4. 青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 山东青岛 266071

收稿日期:2022-12-01 修回日期:2023-01-12 出版日期:2023-09-10 发布日期:2023-02-22
作者简介:
孙文杰(1998—), 女, 硕士研究生, 主要研究方向为海洋环境科学。email: sunwenjie1998@stu.ouc.edu.cn
基金资助:
青岛海洋科学与技术试点国家实验室“十四五”重大项目(2021QNLM040001); 国家自然科学基金项目(41976133)

Transcriptomic analysis of fatty acid metabolism in the Thalassiosira pseudonana under low salinity stress

SUN Wenjie¹^,²(), LI Jiamin¹^,², WANG Hualong³, MI Tiezhu¹^,²^,⁴(), ZHEN Yu¹^,²^,⁴

1. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China
2. Key Laboratory of Marine Environment and Ecology Ministry of Education, Ocean University of China, Qingdao 266100, China
3. College of Marine Life Science, Ocean University of China, Qingdao 266003, China
4. Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

Received:2022-12-01 Revised:2023-01-12 Online:2023-09-10 Published:2023-02-22
Supported by:
Qingdao National Laboratory of Marine Science and Technology(2021QNLM040001); National Natural Science Foundation of China(41976133)

摘要/Abstract

摘要：

文章以假微型海链藻(Thalassiosira pseudonana)转录组测序数据为基础, 对差异表达的功能基因进行GO (gene ontology)和KEGG (kyoto encyclopedia of genes and genomes)富集分析, 以研究低盐胁迫条件对其脂肪酸代谢通路相关功能基因表达的影响。结果发现, 不同盐度培养下的假微型海链藻在生长期的第2天与第4天时细胞中与脂肪酸生物合成、脂肪酸延伸的相关基因(如: ACC1、arm)的表达量相对于生长第1天均显著升高, 与脂肪酸降解相关基因(如: ACADM、ECI1)的表达量无显著变化; 与脂肪酸的生物合成、延长相关基因的表达量在试验组和对照组间存在差异(如: 起重要作用的KASⅠ、ACAA2等基因表达量显著增加, 但也有少数处于下降或者微变动的状态), 与脂肪酸降解过程相关基因的表达量显著上升。

关键词: 假微型海链藻, 脂肪酸代谢, 转录组, 盐胁迫

Abstract:

The expression of fatty acid metabolism-related genes of Thalassiosira pseudonana under different salinities and different growth stages was analyzed by transcriptomic sequencing in this study. A total of 40 coding genes that are related to the fatty acid metabolisms were retrieved from this study with distinct expression levels at different salinity concentrations. The results showed that the expression levels of genes related to fatty acid biosynthesis and elongation (such as ACC1, armB) in T. pseudonana cells were significantly higher at the second and fourth days compared to those at the first day across the salinity gradients, while the expression levels of genes related to fatty acid degradation (such as ACADM, ECI1) did not change significantly. The expression of genes related to fatty acid biosynthesis and elongation in the experimental group were differentially expressed compared to the control group (for example, the expression levels of KASⅠ, ACAA2 and other genes, which play an important role, increased significantly, but a few of them decreased or changed slightly), although the fatty acid degradation related genes were significantly increased in the experimental group. This study improves our understanding of the survival and adaptation strategies of diatoms in the offshore euryhaline environments, and supports the exploration of outbreak and vanishment of harmful algal blooms.

Key words: Thalassiosira pseudonana, fatty acid metabolism, RNA-seq, salinity stress

孙文杰, 李佳岷, 王华龙, 米铁柱, 甄毓. 低盐胁迫下假微型海链藻脂肪酸代谢的转录组学分析[J]. 热带海洋学报, 2023, 42(5): 92-103.

SUN Wenjie, LI Jiamin, WANG Hualong, MI Tiezhu, ZHEN Yu. Transcriptomic analysis of fatty acid metabolism in the Thalassiosira pseudonana under low salinity stress[J]. Journal of Tropical Oceanography, 2023, 42(5): 92-103.

图/表 9

图1

图2

图3

图4

图5

表1

假微型海链藻转录组中与脂肪酸代谢相关的酶以及对应的转录本"

信号通路	酶(蛋白)名称	EC编号	基因ID	基因名称
脂肪酸的生物合成	乙酰-CoA羧化酶(Acetyl-CoA carboxylase)	[EC:6.4.1.2]	THAPS_6770	ACC1
	乙酰-CoA羧化酶(Acetyl-CoA carboxylase)	[EC:6.4.1.2]	THAPSDRAFT_12234	ACC1
	丙二酸单酰CoA转移酶([acyl-carrier-protein] S-malonyltransferase)	[EC:2.3.1.39]	THAPSDRAFT_5219	armB
	β-酮酰-ACP合酶Ⅱ (3-oxoacyl-[acyl-carrier-protein] synthase Ⅱ)	[EC:2.3.1.179]	THAPSDRAFT_961	KASⅡ
			THAPSDRAFT_12152	KAS
			THAPSDRAFT_5021	KASⅠ
			THAPSDRAFT_262879	KAS
	β-酮酰-ACP合酶Ⅲ (3-oxoacyl-[acyl-carrier-protein] synthase Ⅲ)	[EC:1.1.1.180]	THAPSDRAFT_270141	fabH
	长链酰基-CoA合成酶(Long chain acyl-CoA synthetase)	[EC:6.2.1.3]	THAPS_263105	AAE16
			THAPSDRAFT_11953	LACS7
			THAPSDRAFT_270334	AAE15
			THAPSDRAFT_29867	LACS7
			THAPSDRAFT_13156	LACS8
			THAPSDRAFT_25042	LACS7
			THAPSDRAFT_262242	LACS6
	β-羟酰-ACP脱水酶(3-hydroxyacyl-[acyl-carrier-protein] dehydratase)	[EC:4.2.1.59]	THAPSDRAFT_34681	fabZ
	烯酰-ACP还原酶(Enoyl-[acyl-carrier protein] reductase I)	[EC:1.3.1.9]	THAPSDRAFT_32860	fabI
	β-酮酰-ACP还原酶(3-oxoacyl-[acyl-carrier-protein] reductase)	[EC:1.1.1.100]	THAPSDRAFT_270321	fabG
	酰基-ACP去饱和酶(Stearoyl-[acyl-carrier-protein]9-desaturase 2)	[EC:1.14.19.2]	THAPSDRAFT_bd1192	SSI2
脂肪酸的延伸	超长链脂肪酸延伸蛋白(Elongation of fatty acids protein)	[EC:2.3.1.199]	THAPSDRAFT_3741	ELO3
			THAPSDRAFT_728	SPAC1639.01c
			THAPSDRAFT_22362	KCS9
	乙酰-CoA转移酶(Acetyl-CoA acetyltransferase)	[EC:2.3.1.16]	THAPSDRAFT_34809	ACAA2
	超长链酮酰-CoA还原酶(Very-long-chain 3-oxoacyl-CoA reductase)	[EC:1.1.1.330]	THAPSDRAFT_22650	KCR1
	超长链酮酰-CoA还原酶(Very-long-chain 3-oxoacyl-CoA reductase)	[EC:1.1.1.330]	THAPS_35459	ACLA_070510
	超长链羟酰-CoA脱水酶(Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase)	[EC:4.2.1.134]	THAPS_6781	PAS2A
	烯酰-CoA水合酶(Probable enoyl-CoA hydratase 1)	[EC:4.2.1.17]	THAPSDRAFT_34511	ECHS1

表1

图6

图7

图8

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低盐胁迫下假微型海链藻脂肪酸代谢的转录组学分析

Transcriptomic analysis of fatty acid metabolism in the Thalassiosira pseudonana under low salinity stress

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