热带海洋学报 ›› 2024, Vol. 43 ›› Issue (2): 135-139.doi: 10.11978/2023058CSTR: 32234.14.2023058

• 海洋生物学 • 上一篇    下一篇

北部湾柱状角孔珊瑚水螅体次级代谢产物研究

冯广福1,2(), 何洁怡3, 白猛1,2, 易湘茜1,2, 刘昕明1,2, 罗连响3,4, 高程海1,2()   

  1. 1.广西中医药大学海洋药物研究院, 广西 南宁 530200
    2.广西海洋药物重点实验室, 广西 南宁 530200
    3.广东医科大学海洋生物医学研究所, 广东 湛江 524023
    4.广东湛江海洋生物医学研究所, 广东 湛江 524023
  • 收稿日期:2023-05-09 修回日期:2023-06-04 出版日期:2024-03-10 发布日期:2024-03-26
  • 作者简介:

    冯广福 (1995—), 男, 河南省焦作市人, 硕士研究生, 主要从事海洋天然药物研究。email:

  • 基金资助:
    广西壮族自治区自然科学基金项目(2020GXNSFGA297002); 广西壮族自治区重点实验室项目(23-026-03); 广西中医药大学“桂派中医药传承创新团队”项目(2022A007); 广西壮族自治区中医药高层次人才项目(2022C008); 广西壮族自治区研究生教育创新项目(YCSY2022051)

Secondary Metabolites from Hydaranth of Coral Goniopora columna in the Beibu Gulf

FENG Guangfu1,2(), HE Jeyi3, BAI Meng1,2, YI Xiangxi1,2, LIU Xinming1,2, LUO Lianxiang3,4, GAO Chenghai1,2()   

  1. 1. Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
    2. Guangxi Key Laboratory of Marine Drugs, Nanning 530200, China
    3. The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang 524023, China
    4. The Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang 524023, China
  • Received:2023-05-09 Revised:2023-06-04 Online:2024-03-10 Published:2024-03-26
  • Supported by:
    Natural Science Foundation of Guangxi(2020GXNSFGA297002); Guangxi key Laboratory Project(23-026-03); Guangxi University of Chinese Medicine “Guipai Traditional Chinese Medicine Inheritance and Innovation Team” Project(2022A007); High-level Talent of Guangxi Traditional Chinese Medicine(2022C008); Innovation Project of Guangxi Graduate Education(YCSY2022051)

摘要:

为了研究柱状角孔珊瑚(Goniopora columna)水螅体的次级代谢产物, 采用硅胶柱层析、凝胶柱层析及半制备高效液相色谱对柱状角孔珊瑚水螅体粗提物中次级代谢产物进行分离纯化, 利用现代波谱技术鉴定其结构, 并对获得的次级代谢产物进行非小细胞肺癌A549细胞(non-small cell lung cancer A549 cells, NSCLC)的抗癌活性筛选。结果表明, 从柱状角孔珊瑚水螅体中分离鉴定出8种化合物, 分别为alkalgoniopora A (1)、茶碱 (2)、胸腺嘧啶脱氧核糖核苷 (3)、尿嘧啶 (4)、胸腺嘧啶 (5)、pregna-5,20-dien-3β-ol (6)、cinnamic acid (7)和原儿茶酸 (8), 其中化合物1为新发现的生物碱。初步细胞毒活性测试显示, 化合物1、26对NSCLC增殖有较弱的抑制活性, 其余化合物对NSCLC增殖无明显抑制活性。

关键词: 柱状角孔珊瑚, 水螅体, 次级代谢产物, 非小细胞肺癌A549细胞

Abstract:

In order to investigate the secondary metabolites in hydranth of coral Goniopora columna, the ethanol extracted products were isolated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-preparative high-performance liquid chromatography (HPLC). Their structures were identified by modern spectral technology. Meanwhile, the cytotoxicities of 1 ~ 8 were tested against anti-non-small cell lung cancer A549 cells (NSCLC) lines. As a result, a new alkaloid 1 and seven known compounds 2 ~ 8 were isolated and identified as alkalgoniopora A (1), theophylline (2), thymine deoxyribonucleoside (3), uracil (4), thymine (5), pregna-5, 20-dien-3β-ol (6), cinnamic acid (7) and protocatechuic acid (8). Among them, compounds 1, 2 and 6 had weak inhibitory activity on the proliferation of NSCLC, while other compounds had no obvious inhibitory activity on the proliferation of NSCLC. This investigation provides a reference basis for the study of metabolites and exploration of medicinal activity in hydranth of coral Goniopora columna.

Key words: Goniopora columna, hydranth, secondary metabolites, non-small cell lung cancer A549 cells (NSCLC)