热带海洋学报 ›› 2024, Vol. 43 ›› Issue (4): 174-180.doi: 10.11978/2023122CSTR: 32234.14.2023122

• 海洋资源开发 • 上一篇    下一篇

涠洲岛鹿角珊瑚共附生真菌Arachniotus ruber GXIMD 02510的次级代谢产物及抑菌活性研究

王佳熹(), 卢护木, 齐鑫, 高程海, 刘永宏, 罗小卫()   

  1. 广西中医药大学海洋药物研究院, 广西海洋药物重点实验室, 广西 南宁 530200
  • 收稿日期:2023-08-16 修回日期:2023-09-03 出版日期:2024-07-10 发布日期:2024-07-22
  • 作者简介:

    王佳熹(1998—), 男, 甘肃省张掖市人, 硕士研究生, 从事海洋天然药物化学研究。email:

  • 基金资助:
    广西自然科学基金(2024GXNSFFA999005); 广西自然科学基金(2021GXNSFDA075010); 国家自然科学基金(U20A20101); 广西中医药大学第三批“岐黄工程”高层次人才团队培育项目(202407); 桂派杏林青年英才项目(2022C038)

Study on the secondary metabolites from the Weizhou Island coral Acropora austera associated fungus Arachniotus ruber GXIMD 02510 and their antibacterial activity

WANG Jiaxi(), LU Humu, QI Xin, GAO Chenghai, LIU Yonghong, LUO Xiaowei()   

  1. Institute of Marine Drugs, Guangxi University of Chinese Medicine, Guangxi Key Laboratory of Marine Drugs, Nanning 530200, China
  • Received:2023-08-16 Revised:2023-09-03 Online:2024-07-10 Published:2024-07-22
  • Supported by:
    Natural Science Foundation of Guangxi(2024GXNSFFA999005); Natural Science Foundation of Guangxi(2021GXNSFDA075010); National Natural Science Foundation of China(U20A20101); High-Level Talent Training Project Foundation of Guangxi University of Chinese Medicine(202407); High-Level Talent Training Project Foundation of Guangxi University of Chinese Medicine(2022C038)

摘要:

研究涠洲岛来源鹿角珊瑚(Acropora austera)共附生真菌Arachniotus ruber GXIMD 02510的次级代谢产物及其抑菌活性。采用多种现代色谱分离技术对菌株发酵产物进行分离纯化, 利用核磁共振技术和文献数据对比解析化合物的化学结构, 并测定其抑菌活性。从粗提物中共分离鉴定了13个化合物, 分别为auxarthrols B, E, F和H (14)、paradictyoarthrin A (5)、chrysoqueen (6)、ocauxarthrol A (7)、1, 3, 6-三羟基-8-甲基占吨酮 (8)、N-乙酰酪胺(9)、对羟基苯乙酸甲酯(10)、间羟基苯乙酸(11)、L-tenuazonic acid(12)和2, 3, 22, 23-tertrahydroxy-2, 6, 10, 15, 19, 23-hexamethyl-tetracosa-6, 10, 14, 18-tetraene (13)。其中化合物14611对铜绿假单胞菌(Pseudomonas aeruginosa)具一定抑制活性, 最小抑菌浓度(minimal inhibitory concentration, MIC)为0.078~0.312mg·mL-1。本研究丰富了蛛网霉属(Arachniotus)真菌代谢产物结构多样性, 为抗生素研发提供化学实体。

关键词: 涠洲岛, 鹿角珊瑚, Arachniotus ruber, 次级代谢产物, 抑菌活性

Abstract:

The secondary metabolites and antibacterial activities of the Weizhou Island coral Acropora austera associated fungus Arachniotus ruber GXIMD 02510 were investigated. Various modern chromatographic techniques were used to isolate and purify the fermentation products of this strain. Their structures were determined by comprehensive spectroscopic methods, including nuclear magnetic resonance and by comparison with literature data. The antibacterial activity was determined. A total of 13 compounds were obtained and characterized, which were auxarthrols B, E, F, and H (14), paradictyoarthrin A (5), chrysoqueen (6), ocauxarthrol A (7), 1, 3, 6-trihydroxy-8-methylxanthone (8), N-acetyltyramine (9), methyl 2-(4-hydroxyphenyl)acetate (10), m-hydroxylphenylacitic acid (11), L-tenuazonic acid (12), and 2, 3, 22, 23-tertrahydroxy-2, 6, 10, 15, 19, 23-hexamethyl-tetracosa-6, 10, 14, 18-tetraene (13). Antibacterial activity test showed that compounds 1, 46, and 11 showed inhibitory activity against Pseudomonas aeruginosa, and the minimum inhibitory concentration (MIC) values were ranging from 0.078 mg·mL-1 to 0.312 mg·mL-1. This study will expand the chemical diversity of the genus Arachniotus and also provide chemical entities for the development of antibiotics.

Key words: Weizhou Island, Acropora austera, Arachniotus ruber, secondary metabolites, antibacterial activity