绿潮藻浒苔对赤潮藻中肋骨条藻化感抑制作用初探*

doi:10.11978/j.issn.1009-5470.2014.05.004

热带海洋学报 ›› 2014, Vol. 33 ›› Issue (5): 28-34.doi: 10.11978/j.issn.1009-5470.2014.05.004cstr: 32234.14.j.issn.1009-5470.2014.05.004

绿潮藻浒苔对赤潮藻中肋骨条藻化感抑制作用初探^*

崔峰, 涂文斌, 王亦冰, 吴海龙, 张建恒, 霍元子, 贾睿, 何培民

上海海洋大学水产与生命学院海洋科学研究院, 上海 201306

收稿日期:2013-10-13 修回日期:2014-03-14 出版日期:2014-09-29 发布日期:2014-09-29
通讯作者: 通信作者：贾睿。E-mail: rjia@shou.edu.cn
作者简介:作者简介：崔峰(1990~), 男, 安徽省亳州市人, 硕士研究生, 研究方向为天然产物化学分析。E-mail: cuifeng1127@sina.com
基金资助:
国家科技支撑项目(2012BAC07B03); 上海市科委项目(10391901900); 上海市教委科研创新项目(11YZ149); 教育部留学回国人员科研启动基金项目; 国家海洋局东海分局开放基金项目(MATHAB20100205、MATHAB 20100309); 上海海洋大学研究生科研基金项目(A-0209-13-0900002)

Allelopathic effects of green tide algae Ulva prolifera on red tide algae Skeletonema costatum

CUI Feng, TU Wen-bin, WANG Yi-bing, WU Hai-long, ZHANG Jian-heng, HUO Yuan-zi, JIA Rui, HE Pei-min

Shanghai Ocean University, Institute of Marine Science, Shanghai 201306, China

Received:2013-10-13 Revised:2014-03-14 Online:2014-09-29 Published:2014-09-29

摘要/Abstract

摘要： 研究了绿潮藻浒苔Ulva prolifera对赤潮藻中肋骨条藻Skeletonema costatum生长的化感抑制作用。分别测定了不同浒苔新鲜藻体密度、不同浒苔藻体干粉末含量、不同浒苔新鲜藻体培养过滤液体积对中肋骨条藻生长的抑制效果。结果发现, 第12天共培养系统中, 各浒苔新鲜藻体密度组对中肋骨条藻生长抑制率均高达100%, 其中5g·L^-1密度组第8天对中肋骨条藻生长的抑制已达到100%; 第12天1.2g·L^-1浒苔藻体干粉末含量组对中肋骨条藻生长抑制率也可达到50%左右, 表明绿潮藻浒苔对中肋骨条藻生长抑制作用具有量效关系, 且对中肋骨条藻生长不仅存在营养竞争抑制作用, 而且还具有化感物质抑制作用。进一步采用添加浒苔新鲜藻体培养过滤液实验发现, 一次性添加浒苔培养过滤液对中肋骨条藻生长抑制作用不十分明显, 而半连续添加浒苔培养过滤液对中肋骨条藻生长抑制效果十分明显, 其中第12天对中肋骨条藻生长抑制可达75%左右, 可见浒苔藻体内的化感物质是逐步向环境中分泌的。该研究为进一步研究绿潮藻对赤潮藻化感抑制效应量效关系奠定了基础。

关键词: 浒苔, 中肋骨条藻, 化感抑制作用

Abstract: Allelopathic effects of green tide alga Ulva prolifera on the proliferation of red tide algae Skeletonema costatum were studied. The fresh tissue, dry powder and culture medium filtrate of U. prolifera were used for inhibiting the proliferation of S. costatum. Results showed that the fresh tissue of U. prolifera could inhibit significantly the proliferation of S. costatun. In all groups with the fresh tissue, the inhibition rate was as high as 100% after co-culturing for 12 days. As for the groups with the dry powder, the inhibition rate in the 1.2 g·L^-1 group reached 50% after co-culturing for 12 days. It indicated that the inhibition of green tide algae on S. costatun proliferation presented a concentration-response relationship, and that there was not only nutrient competition inhibitory but also allelochemicals inhibition. Further studies showed that there was no significant inhibition of culture medium filtrate on the proliferation of S. costatum in the experiment with one-time adding culture medium filtrate of U. prolifera, while the proliferation of S. costatum was significantly inhibited in the experiment with semi-continuously adding culture medium filtrate of U. prolifera. The inhibition rate for semi-continuously adding culture medium filtrate of U. prolifera was 75% after culturing for 12 days. It indicated that allelochemicals in U. prolifera was gradually released to the environment. This research laid a solid foundation for further research on concentration-response relationship between U. prolifera and S. costatum.

Key words: 浒苔, 中肋骨条藻, 化感抑制作用

中图分类号:

P735

崔峰, 涂文斌, 王亦冰, 吴海龙, 张建恒, 霍元子, 贾睿, 何培民. 绿潮藻浒苔对赤潮藻中肋骨条藻化感抑制作用初探^*[J]. 热带海洋学报, 2014, 33(5): 28-34.

CUI Feng, TU Wen-bin, WANG Yi-bing, WU Hai-long, ZHANG Jian-heng, HUO Yuan-zi, JIA Rui, HE Pei-min. Allelopathic effects of green tide algae Ulva prolifera on red tide algae Skeletonema costatum[J]. Journal of Tropical Oceanography, 2014, 33(5): 28-34.

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绿潮藻浒苔对赤潮藻中肋骨条藻化感抑制作用初探^*

Allelopathic effects of green tide algae Ulva prolifera on red tide algae Skeletonema costatum

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