不同温度下抑食金球藻、中肋骨条藻和海洋卡盾藻间的相互作用

doi:10.11978/2020154

热带海洋学报 ›› 2021, Vol. 40 ›› Issue (3): 124-131.doi: 10.11978/2020154CSTR: 32234.14.2020154

不同温度下抑食金球藻、中肋骨条藻和海洋卡盾藻间的相互作用

郑承志^1,²(), 左丽明³(), 马旺³, 朱琴³, 王火火³, 吕颂辉^1,², 陈亨^1,², 黄凯旋^1,²()

1.暨南大学赤潮与海洋生物学研究中心, 广东广州 510632
2.水体富营养化与赤潮防治广东普通高校重点实验室, 广东广州 510632
3.河北省水文工程地质勘查院, 河北石家庄 050021

收稿日期:2020-12-29 修回日期:2021-02-09 出版日期:2021-05-10 发布日期:2021-02-10
通讯作者: 黄凯旋
作者简介:郑承志(1994—), 男, 广东省湛江市人, 硕士, 海洋生物学, email: lambert@stu2018.jnu.edu.cn;|左丽明(1980—), 女, 河北省沧州市人, 高级工程师, 海洋地质学, email: zlmchina@126.com。
基金资助:
国家自然科学基金(41876173);河北省自然资源厅项目(454-0503-JBN-XCLS)

Interactions among Aureococcus anophagefferens, Skeletonema costatum, and Chattonella marina under different temperatures

ZHENG Chengzhi^1,²(), ZUO Liming³(), MA Wang³, ZHU Qin³, WANG Huohuo³, LÜ Songhui^1,², CHEN Heng^1,², HUANG Kaixuan^1,²()

1. Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, China
2. Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes, Guangzhou 510632, China
3. Hebei Prospecting Institute of Hydro-engineering Geology, Shijiazhuang 050021, China

Received:2020-12-29 Revised:2021-02-09 Online:2021-05-10 Published:2021-02-10
Contact: HUANG Kaixuan
Supported by:
National Natural Science Foundation of China(41876173);Foundation of Department of Natural Resources of Heibei Province(454-0503-JBN-XCLS)

摘要/Abstract

摘要：

本文选取抑食金球藻(Aureococcus anophagefferens)、中肋骨条藻(Skeletonema costatum)和海洋卡盾藻(Chattonella marina)三种藻在20℃和25℃下进行单种、两种或三种藻间的培养实验, 探究在不同温度下藻种间的相互作用和竞争优势。单种培养结果显示抑食金球藻和中肋骨条藻对温度25℃敏感, 其环境容量(K)被显著降低; 而海洋卡盾藻的內禀增长率(r)在25℃下显著升高, 但K保持不变。在共培养中, 海洋卡盾藻在20℃下因抑食金球藻添加致死, 但在25℃处理下其K值上升120%。虽然中肋骨条藻的r在两个温度下均受海洋卡盾藻添加的促进, 但其K值从20℃下的上升43%转变为25℃下的降低48%。温度变化对于抑食金球藻和中肋骨条藻共培养的结果无明显影响, 中肋骨条藻的K均上升40%, 而抑食金球藻的K值均下降60%~70%。结果说明, 种间竞争除了共培养藻固有的相互作用关系因素外, 也受到微藻间温度适应性差异的影响。在20℃条件下三种藻混合, 抑食金球藻K的抑制率达到最高为79%, 而中肋骨条藻K的促进率达到最高为108%, 这可能表明三者之间的相互作用存在协同效应。

关键词: 抑食金球藻, 中肋骨条藻, 海洋卡盾藻, 藻华, 种间竞争, 温度

Abstract:

Mono-, bi-, and tri-algal cultures were applied to Aureococcus anophagefferens, Skeletonema costatum, and Chattonella marina under two temperatures of 20℃ and 25℃, to investigate species-specific interaction and competition in different temperatures. Single cultures showed the environmental carry capacity (K) for A. anophagefferens and S. costatum were significantly reduced by increased temperature, indicating they are sensitive to rising temperature. In contrast, the inherent growth rate (r) for C. marina significantly increased at 25℃ whereas K remained the same as that at 20℃. In contrast to the death under A. anophagefferens addition at 20℃, K of C. marina increased 120% at 25℃. Although r of S. costatum was promoted by C. marina addition at both temperatures, its K showed from 43% increase to 48% decrease from 20℃ to 25℃. The change of temperature did not influence the results of co-culture of S. costatum and A. anophagefferens with 40% increases and 60%-70% decreases of K at both temperatures. The results of bi-cultures indicated that interspecies competition is not only influenced by fixed interaction relationship of co-cultured species, but also by difference of algal temperature adaptability. In tri-algal cultures at 20℃, the inhibition rate of A. anophagefferens and the stimulation rate of S. costatum both reached the highest of 79% and 108%, respectively, which probably illuminated a synergistic effect within three algal interaction.

Key words: Aureococcus anophagefferens, Skeletonema costatum, Chattonella marina, interspecies competition, algae bloom, temperature

中图分类号:

郑承志, 左丽明, 马旺, 朱琴, 王火火, 吕颂辉, 陈亨, 黄凯旋. 不同温度下抑食金球藻、中肋骨条藻和海洋卡盾藻间的相互作用[J]. 热带海洋学报, 2021, 40(3): 124-131.

ZHENG Chengzhi, ZUO Liming, MA Wang, ZHU Qin, WANG Huohuo, LÜ Songhui, CHEN Heng, HUANG Kaixuan. Interactions among Aureococcus anophagefferens, Skeletonema costatum, and Chattonella marina under different temperatures[J]. Journal of Tropical Oceanography, 2021, 40(3): 124-131.

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处理	初始密度/(cells·mL^-1)			混合比例
处理	抑食金球藻	中肋骨条藻	海洋卡盾藻	混合比例
单种	20×10⁵	2.5×10⁵	2.5×10³	N/A
混合1	40×10⁵	2.5×10⁵	N/A	16∶1
混合2	N/A	2.5×10⁵	750	33∶1
混合3	40×10⁵	N/A	750	530∶1
混合4	25×10⁵	2.5×10⁵	750	333∶33∶1

温度/℃	种名	处理	环境容纳量K/(×10⁵cells·mL^-1)	內禀增长率r	生长拐点T_p/d
20	抑食金球藻	单种	161.63±3.40	0.24±0.02	8.54±0.63
		混合1	48.09±15.50	0.29±0.07	23.83±4.76
		混合3	83.31±71.28	0.24±0.07	13.46±3.34
		混合4	34.72±4.80	0.43±0.01	46.00±0.85
	海洋卡盾藻	单种	0.25±0.01	0.26±0.02	7.77±0.68
		混合2	0.09±0.02	0.20±0.02	13.2±1.11
		混合3	0.07±0.04	0.23±0.06	49.00±7.67
		混合4	0.10±0.02	0.14±0.02	18.50±1.32
	中肋骨条藻	单种	16.66±0.53	0.45±0.02	4.04±0.15
		混合1	23.28±3.04	0.45±0.03	10.20±0.46
		混合2	23.84±1.99	0.52±0.02	9.12±0.25
		混合4	34.73±4.79	0.43±0.01	11.77±0.3
25	抑食金球藻	单种	65.21±4.93	0.63±0.17	6.89±1.78
		混合1	27.10±3.57	0.19±0.02	19.42±1.34
		混合3	19.57±2.68	0.24±0.03	11.42±1.03
		混合4	18.57±6.40	0.17±0.04	13.00±2.44
	海洋卡盾藻	单种	0.25±0.01	0.40±0.03	5.10±0.35
		混合2	0.05±0.01	0.15±0.02	10.07±1.2
		混合3	0.55±0.50	0.20±0.02	21.85±3.32
		混合4	0.13±0.03	0.15±0.02	18.00±1.73
	中肋骨条藻	单种	13.37±0.33	0.47±0.01	3.23±0.07
		混合1	18.70±2.56	0.49±0.02	8.88±0.27
		混合2	6.99±0.39	0.52±0.02	6.75±0.18
		混合4	14.94±1.22	0.63±0.10	7.98±0.64

不同温度下抑食金球藻、中肋骨条藻和海洋卡盾藻间的相互作用

Interactions among Aureococcus anophagefferens, Skeletonema costatum, and Chattonella marina under different temperatures

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