除草剂乙草胺胁迫下两种软珊瑚生理响应

doi:10.11978/2022183

摘要/Abstract

摘要：

文章以乙草胺(acetochlor, ACT)为环境胁迫因子, 利用不同浓度的乙草胺对肉质软珊瑚(Sarcophyton sp.)和短指软珊瑚(Sinularia sp.)进行长期和短期胁迫, 以研究胁迫下的珊瑚生理指标变化情况。研究结果显示, 肉质软珊瑚和短指软珊瑚受到不同浓度乙草胺胁迫后, 珊瑚的叶绿素a和虫黄藻含量呈现先升高后降低的趋势, 超氧化物歧化酶(superoxide dismutase, SOD)和过氧化氢酶(catalase, CAT)活性持续升高然后降低并保持低活性水平, 碱性磷酸酶(alkaline phosphatase, AKP)活性先升高后逐渐降低, 谷胱甘肽巯基转移酶(glutathione s-transferase, GST)活性升高并保持在较高水平。胁迫后肉质软珊瑚的共生虫黄藻多样性变低, 而短指软珊瑚的共生虫黄藻则是丰富度变低。丰度高的虫黄藻都属于系群C, 而短指软珊瑚G系群丰度升高。胁迫后软珊瑚Cyanobacteria(蓝细菌)丰度逐渐增加, Firmicutes(厚壁菌门)丰度逐渐减少。以上结果表明, 肉质软珊瑚(Sarcophyton sp.)和短指软珊瑚(Sinularia sp.)在受到乙草胺胁迫后, 其珊瑚生理指标产生了较大变化。

关键词: 软珊瑚, 乙草胺, 虫黄藻, 生理指标

Abstract:

In this paper, using acetochlor (ACT) as the stress environment, the long-term and short-term stress of Sarcophyton sp. and Sinularia sp. with different concentrations of acetochlor were used to study the stress changes in coral physiological indicators. Studies have shown that the chlorophyll a and zooxanthellae contents of succulent soft corals and brachydactyly soft corals increased first and then decreased after different concentrations of acetochlor stress. Superoxide dismutase (SOD) and Catalase (CAT) activity will continue to increase and then decrease and maintain a low level, Alkaline Phosphatase (AKP) activity will first increase and then gradually decrease, Glutathione S-Transferase (GST) activity increased and maintained at a high level. The symbiotic zooxanthellae diversity of succulent soft corals became lower, while the abundance of symbiotic zooxanthellae of Brachydactyly soft corals became lower. The zooxanthellae with high abundance all belonged to group C, while the abundance of group G of Brachydactyly was increased. The abundance of soft coral Cyanobacteria gradually increased and the abundance of Firmicutes gradually decreased after stress. The above studies showed that acetochlor had great changes in coral physiological indicators after the stress of Sarcophyton sp. and Sinularia sp..

Key words: soft coral, acetochlor, zooxanthellae, Physiological indicators

郁强, 董娴娴, 李长青, 张欣, 赵国理, CHIN Yaoxian, 王沛政. 除草剂乙草胺胁迫下两种软珊瑚生理响应[J]. 热带海洋学报, 2023, 42(4): 133-145.

YU Qiang, DONG Xianxian, LI Changqing, ZHANG Xin, ZHAO Guoli, CHIN Yaoxian, WANG Peizheng. Physiological responses of two soft corals to herbicide acetochlor stress[J]. Journal of Tropical Oceanography, 2023, 42(4): 133-145.

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实验类别	Shannon指数	Simpson指数	Ace指数	Chao指数	Coverage指数
S0S0	0.165±0.003	0.945±0.001	7.4±1.38	6.5±0.5	1
S24S0.5	0.347±0.051*	0.867±0.021*	4.5±0.21*	8.5±0.5	1
S24S4	0.488±0.156*	0.798±0.073*	4.6±0.15	7.0±1.0	1
S24S8	0.279±0.031	0.902±0.009	4.0±0.14*	8.5±0.5	1
S72S0.5	0.273±0.024*	0.895±0.008*	3.0±0.95*	6.5±0.5	1
S72S4	0.287±0.005*	0.890±0.001*	3.0±1.13*	6.5±0.5	1
S72S8	0.223±0.025*	0.922±0.012*	7.0±1.07	7.0±0.6	1

实验类别	Shannon指数	Simpson指数	Ace指数	Chao指数	Coverage指数
M0M0	1.042±0.006	0.488±0.009	4.0±0.76	7.0±1.0	1
M24M0.5	1.049±0.025	0.499±0.002	4.5±0.38	8.0±0.2	1
M24M4	1.029±0.008	0.503±0.005	8.0±1.23	8.0±0.1	1
M24M8	0.759±0.292*	0.657±0.155*	9.5±1.5	9.0±1.0*	1
M72M0.5	1.066±0.055	0.498±0.018	4.0±0.28	8.0±0.3	1
M72M4	1.018±0.006	0.506±0.005	4.0±0.09	7.5±0.5	1
M72M8	0.749±0.283*	0.660±0.155*	9.1±0.06*	8.5±0.5*	1

实验类别	Shannon指数	Simpson指数	Ace指数	Chao指数	Coverage指数
M0M0	5.116±0.267	0.019±0.003	743.1±119.5	744.2±121.8	1
M24M0.5	4.257±0.468*	0.079±0.023*	580.0±140.3*	580.6±137.1*	1
M24M4	3.170±0.046*	0.221±0.019*	400.1±26.8*	401.1±29.5*	1
M24M8	3.595±0.541*	0.189±0.078*	763.5±99.5	768.6±64.9	1
M72M0.5	3.932±0.248	0.087±0.001*	519.1±51.0	529.9±45.1	1
M72M4	3.375±0.193*	0.158±0.004*	523.1±45.5	527.4±42.1	1
M72M8	5.023±1.039	0.032±0.004	1065.2±49.7	1075.6±65.4	1