濒临死亡哈德掌状藻沉降行为的时频特征

doi:10.11978/2024202

热带海洋学报 ›› 2025, Vol. 44 ›› Issue (3): 85-94.doi: 10.11978/2024202CSTR: 32234.14.2024202

濒临死亡哈德掌状藻沉降行为的时频特征

石天应¹^,²(), 陆家昌²^,³(), 庄军莲²^,³, 赖俊翔²^,³, 李朗²^,³

1.广西大学资源环境与材料学院, 广西南宁 530004
2.广西科学院, 广西海洋科学院, 广西近海海洋环境科学重点实验室, 广西南宁 530007
3.北部湾海洋产业研究院, 广西防城港 538000

收稿日期:2024-10-26 修回日期:2024-12-04 出版日期:2025-05-10 发布日期:2025-06-04
通讯作者: 陆家昌
作者简介:
石天应(1999—), 女, 湖南省长沙市人, 硕士研究生, 从事海洋硅藻沉降研究。email: sty2303@163.com
基金资助:
广西自然科学基金(2025GXNSFAA069768)

Time-frequency characteristics of the sinking behavior in dying Palmeria hardmaniana

SHI Tianying¹^,²(), LU Jiachang²^,³(), ZHUANG Junlian²^,³, LAI Junxiang²^,³, LI Lang²^,³

1. Guangxi University (School of Resources, Environment and Materials), Nanning 530004, China
2. Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning 530007, China
3. Beibu Gulf Marine Industry Research Institute, Fangchenggang 538000, China

Received:2024-10-26 Revised:2024-12-04 Online:2025-05-10 Published:2025-06-04
Contact: LU Jiachang
Supported by:
Guangxi Natural Science Foundation(2025GXNSFAA069768)

摘要/Abstract

摘要： 哈德掌状藻(Palmeria hardmaniana)是近岸常见大型浮游硅藻, 其活细胞因为具有沉降行为, 能主动调节自身沉降速度, 所以与死细胞的沉降过程存在明显差异。除了活细胞和死细胞外, 海洋中还存在因为环境胁迫导致的濒临死亡的硅藻细胞, 这类细胞的沉降行为目前尚不清楚。本研究以热处理获得的濒临死亡的哈德掌状藻作为研究对象, 以指数生长期的健康细胞为参照, 通过摄像技术追踪记录这两类细胞的沉降过程, 借助连续小波分析工具提取它们沉降行为的时频特征, 探讨濒临死亡哈德掌状藻细胞沉降行为的特点。结果表明, 热处理导致的濒临死亡的哈德掌状藻细胞光合系统受到严重损伤。较之健康细胞, 热处理细胞具有更快的沉降速度和更远的沉降距离。濒临死亡和健康哈德掌状藻细胞的沉降行为均是一种频率小于400mHz的行为, 这两类细胞的行为在128~400mHz频段存在联系, 推测该频段包含哈德掌状藻的基础沉降行为。濒临死亡细胞的沉降行为是一种高频、高耗能调节行为, 集中发生在300~400mHz; 健康细胞的沉降行为是一种低频、低耗能调节行为, 主要发生在100~350mHz。濒临死亡细胞在观察期间进行了更少的主动调节, 且调节时间更短。

关键词: 哈德掌状藻, 沉降行为, 时频特征, 濒临死亡细胞

Abstract:

Palmeria hardmaniana, a common large planktonic diatom in coastal waters, shows sinking behavior in its living cells and actively regulates its own sinking rate, which is significantly different from the sinking process of its dead cells. Besides living and dead cells, the ocean also contains diatom cells that are in the process of dying due to environmental stress. The sinking behavior of these dying cells remains unclear. The subject of this study is the sinking behavior of P. hardmaniana on the brink of death obtained by heat treatment, with healthy cells in the exponential growth phase serving as the reference. To explore the characteristics of the sinking behavior of dying P. hardmaniana cells, this study tracked and recorded their sedimentation process using videography and employed continuous wavelet analysis to extract the time-frequency characteristics of their sinking behavior. The results show that the heat treatment severely damaged the photosynthetic system of the dying P. hardmaniana cells, leading to faster sedimentation velocities and longer sedimentation distances compared to the healthy cells. The sinking behaviors of both the dying and healthy P. hardmaniana cells fall within the frequency range below 400 mHz. There is a correlation between the behaviors of these two types of cells in the frequency range of 128~400 mHz, suggesting that this range may encompass the fundamental sinking behavior of P. hardmaniana. The sinking behavior of the dying cells is characterized by high-frequency, high-energy regulation, primarily occurring in the frequency range of 300~400 mHz. In contrast, the sinking behavior of the healthy cells is marked by low-frequency, low-energy regulation, predominantly observed between 100~350 mHz. In comparison to their healthy counterparts, the dying cells exhibited fewer active regulatory events and each regulatory event lasted for a shorter duration during the observation period.

Key words: Palmeria hardmaniana, sinking behavior, time-frequency characteristics, dying cells

中图分类号:

Q948.8

石天应, 陆家昌, 庄军莲, 赖俊翔, 李朗. 濒临死亡哈德掌状藻沉降行为的时频特征[J]. 热带海洋学报, 2025, 44(3): 85-94.

SHI Tianying, LU Jiachang, ZHUANG Junlian, LAI Junxiang, LI Lang. Time-frequency characteristics of the sinking behavior in dying Palmeria hardmaniana[J]. Journal of Tropical Oceanography, 2025, 44(3): 85-94.

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濒临死亡哈德掌状藻沉降行为的时频特征

Time-frequency characteristics of the sinking behavior in dying Palmeria hardmaniana

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