盐度对广盐型聚球藻K1生长及转录组的影响*

doi:10.11978/2021040

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (2): 159-169.doi: 10.11978/2021040CSTR: 32234.14.2021040

盐度对广盐型聚球藻K1生长及转录组的影响*

廖莹^1,²(), 夏晓敏^1,²()

1. 热带海洋生物资源与生态重点实验室(中国科学院南海海洋研究所), 广东广州 510301
2. 中国科学院大学, 北京 100049

收稿日期:2021-04-06 修回日期:2021-05-01 出版日期:2022-03-10 发布日期:2021-05-03
通讯作者: 夏晓敏
作者简介:廖莹(1994—), 女, 河北省衡水市人, 硕士研究生, 从事海洋生态学研究。email: liaoying18@mails.ucas.ac.cn
基金资助:
国家自然科学基金(41906131);中国科学院海洋大科学研究中心重点部署项目(COMS2020Q09);广东省自然科学基金面上项目(2019A1515011340)

Effects of salinity on the growth and transcriptome of euryhaline Synechococcus sp. K1*

LIAO Ying^1,²(), XIA Xiaomin^1,²()

1. Key Laboratory of Tropical Marine Bio-resources and Ecology (South China Sea Institute of Oceanology, Chinese Academy of Sciences), Guangzhou 510301, China
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2021-04-06 Revised:2021-05-01 Online:2022-03-10 Published:2021-05-03
Contact: XIA Xiaomin
Supported by:
Nature Science Foundation of China(41906131);Key Deployment Program of Center for Ocean Mega-Science, Chinese Academy of Sciences(COMS2020Q09);Guangdong Basic and Applied Basic Research Foundation(2019A1515011340)

摘要/Abstract

摘要：

聚球藻是一类分布广泛、数量巨大的微微型浮游植物, 作为蓝藻的代表类群之一, 广泛分布于海洋以及河口区域, 并且具有丰富的色素多样性和遗传多样性。聚球藻根据盐度适应能力可划分为严格海洋型聚球藻和广盐型聚球藻。文章对分离自珠江口的聚球藻K1和南海寡营养海域的聚球藻YX02-1在不同盐度条件下的生长状况进行对比, 并根据ropC1标记基因分析K1和YX02-1的分类地位, 发现K1为广盐型聚球藻, 其在不同盐度下(10‰、13‰、18‰、25‰、33‰)均能生长; 而YX02-1为严格海洋型聚球藻, 在低于13‰的盐度下无法存活, 与其在河口的分布特征相一致。转录组分析显示, 低盐条件下广盐型聚球藻中合成渗透压调节分子的基因(ggpS、SPS、stpA)表达量明显下调, 而膜通道蛋白glzT基因的表达显著上调, 说明广盐型聚球藻耐低盐机制主要是通过减少细胞内渗透压相关小分子合成和增加膜通道蛋白, 提高小分子物质外排来达到细胞内外渗透压平衡。此外, 盐度也会影响广盐型聚球藻的光合作用和代谢水平, 其中参与光合作用的产能基因(ATPF0B、ATPF0A、ATPF1D、ATPF1A)和色素蛋白基因(cpcA、cpcB)表达量在低盐条件下均显著下降, 同时无机氮利用相关基因表达上调。低盐条件下, 渗透压相关小分子的需求减少, 可以使更多的碳源用于支持生长, 同时无机氮的吸收增强, 这两者可能是低盐条件下广盐型聚球藻具有较高生长的原因。

关键词: 广盐型聚球藻, 转录组分析, 盐度

Abstract:

Synechococcus, which is one of the representative groups of picocyanobacteria, is widely distributed in global oceans and estuaries. It has high phenotypic (pigment) and genetic diversity. According to the level of ability to deal with variation in salinity, Synechococcus can be divided into the strictly marine type and euryhaline type. In this study, we compared the growth of Synechococcus sp. K1 (isolated from the Pearl River estuary) and Synechococcus sp. YX02-1 (isolated from the South China Sea oligotrophic water) under a series of salinity gradient conditions. We also conducted phylogenetic analysis of K1 and YX02-1 based on rpoC1 gene sequences. We found that euryhaline Synechococcus sp. K1 could grow in all salinity levels, while strictly marine Synechococcus sp. YX02-1 was unable to survive below 13‰, which is consistent with the distribution characteristics in the estuary. Transcriptome analysis showed that the expression of genes (ggpS, SPS, stpA) that synthesize osmotic pressure regulator molecules in euryhaline Synechococcus were significantly down-regulated, while the gene expression of the membrane channel protein glzT was significantly up-regulated under low-salinity condition. This suggests that the low-salt tolerance mechanism of euryhaline Synechococcus mainly includes reducing the synthesis of small molecules related to intracellular osmotic pressure and increasing membrane channel proteins to improve the efflux of small molecules. In addition, salinity affected the photosynthesis and metabolism levels of euryhaline Synechococcus. The expression of photosynthesis energy-saving genes (ATPF0B, ATPF0A, ATPF1D, ATPF1A) and pigment protein genes (cpcA, cpcB) were significantly down-regulated under low-salinity condition, and the expression of inorganic nitrogen utilization-related genes were up-regulated. Under low-salinity condition, the demand for small molecules related to osmotic pressure is reduced, which can make more carbon sources to support growth. Meanwhile, the absorption of inorganic nitrogen is enhanced. These two reasons may be responsible for the higher growth of Synechococcus under low-salinity condition.

Key words: Euryhaline Synechococcus, transcriptome analysis, salinity

中图分类号:

P735.121

廖莹, 夏晓敏. 盐度对广盐型聚球藻K1生长及转录组的影响*[J]. 热带海洋学报, 2022, 41(2): 159-169.

LIAO Ying, XIA Xiaomin. Effects of salinity on the growth and transcriptome of euryhaline Synechococcus sp. K1*[J]. Journal of Tropical Oceanography, 2022, 41(2): 159-169.

图/表 10

表1

图1

图2

图3

图4

图5

图6

图7

图8

图9

参考文献 25

[1]	路荣昭, 王淑芝, 关志英, 等, 1991. 聚球藻(Synechococcus leopoliensis 625)藻胆体-类囊体膜光谱特性和光能传递的研究[J]. 水生生物学报, 15(4): 368-371.
	LU RONGZHAO, WANG SHUZHI, GUAN ZHIYING, et al, 1991. Studies on the spectroscopic properties and energy transfer of phycobilisome-thylakoid of Synechococcus leopoliensis 625[J]. Acta Hydrobiologica Sinica, 15(4): 368-371. (in Chinese with English abstract)
[2]	马英, 焦念志, 2004. 聚球藻(Synechococcus)分子生态学研究进展[J]. 自然科学进展, 14(9): 967-972.
	MA YING, JIAO NIANZHI, 2004. Progress in molecular ecology of Synechococcus[J]. Progress in Natural Science, 14(9): 967-972. (in Chinese with English abstract)
[3]	AHLGREN N A, ROCAP G, 2006. Culture isolation and culture-independent clone libraries reveal new marine Synechococcus ecotypes with distinctive light and N physiologies[J]. Applied and Environmental Microbiology, 72(11): 7193-7204. doi: 10.1128/AEM.00358-06
[4]	CALLIERI C, STOCKNER J G, 2002. Freshwater autotrophic picoplankton: a review[J]. Journal of Limnology, 61(1): 1-14.
[5]	CELEPLI N, SUNDH J, EKMAN M, et al, 2017. Meta-omic analyses of Baltic Sea cyanobacteria: diversity, community structure and salt acclimation[J]. Environmental Microbiology, 19(2): 673-686. doi: 10.1111/emi.2017.19.issue-2
[6]	DESHNIUM P, LOS D A, HAYASHI H, et al, 1995. Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress[J]. Plant Molecular Biology, 29(5): 897-907. doi: 10.1007/BF00014964
[7]	EVERROAD R C, WOOD A M, 2012. Phycoerythrin evolution and diversification of spectral phenotype in marine Synechococcus and related picocyanobacteria[J]. Molecular Phylogenetics and Evolution, 64(3): 381-392. doi: 10.1016/j.ympev.2012.04.013
[8]	FULLER N J, MARIE D, PARTENSKY F, et al, 2003. Clade-specific 16S ribosomal DNA oligonucleotides reveal the predominance of a single marine Synechococcus clade throughout a stratified water column in the Red Sea[J]. Applied and Environmental Microbiology, 69(5): 2430-2443. doi: 10.1128/AEM.69.5.2430-2443.2003
[9]	GUILLARD R R L, RYTHER J H, 1962. Studies of marine planktonic diatoms: Ⅰ. Cyclotella nana hustedt, and Detonula confervacea (cleve) gran[J]. Canadian Journal of Microbiology, 8(2): 229-239. doi: 10.1139/m62-029
[10]	HERDMAN M, CASTENHOLZ RW, WATERNURY J B, et al, 2001. Form-genus ⅩⅢ. Synechococcus[M]//BOONE D R, CASTENHOLZ R W. Bergey’s Manual of Systematic Bacteriology. New York, NY: Springer-Verlag, 508-512.
[11]	KUMAR S, STECHER G, TAMURA K, 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution, 33(7): 1870-1874. doi: 10.1093/molbev/msw054
[12]	LUDWIG M, BRYANT D A, 2012. Synechococcus sp. strain PCC 7002 transcriptome: acclimation to temperature, salinity, oxidative stress, and mixotrophic growth conditions[J]. Frontiers in Microbiology, 3: 354, doi: 10.3389/fmicb.2012. 00354. doi: 10.3389/fmicb.2012. 00354
[13]	MARSAN D, PLACE A, FUCICH D, et al, 2017. Toxin-Antitoxin systems in estuarine Synechococcus strain CB0101 and their transcriptomic responses to environmental stressors[J]. Frontiers in Microbiology, 8: 1213, doi: 10.3389/fmicb.2017.01213. doi: 10.3389/fmicb.2017.01213
[14]	MOORE L R, POST A F, ROCAP G, et al, 2002. Utilization of different nitrogen sources by the marine cyanobacteria Prochlorococcus and Synechococcus[J]. Limnology and Oceanography, 47(4): 989-996. doi: 10.4319/lo.2002.47.4.0989
[15]	MÜHLING M, FULLER N J, MILLARD A, et al, 2005. Genetic diversity of marine Synechococcus and co-occurring cyanophage communities: evidence for viral control of phytoplankton[J]. Environmental Microbiology, 7(4): 499-508. doi: 10.1111/emi.2005.7.issue-4
[16]	PALENIK B, BRAHAMSHA B, LARIMER F W, et al, 2003. The genome of a motile marine Synechococcus[J]. Nature, 424(6952): 1037-1042. doi: 10.1038/nature01943
[17]	ROCAP G, DISTEL D L, WATERBURY J B, et al, 2002. Resolution of Prochlorococcus and Synechococcus ecotypes by using 16S-23S ribosomal DNA internal transcribed spacer sequences[J]. Applied and Environmental Microbiology, 68(3): 1180-1191. doi: 10.1128/AEM.68.3.1180-1191.2002
[18]	SIX C, THOMAS J C, GARCZAREK L, et al, 2007. Diversity and evolution of phycobilisomes in marine Synechococcus spp.: a comparative genomics study[J]. Genome Biology, 8(12): R259. doi: 10.1186/gb-2007-8-12-r259
[19]	WANG KUI, WOMMACK K E, CHEN FENG, 2011. Abundance and distribution of Synechococcus spp. and cyanophages in the Chesapeake Bay[J]. Applied and Environmental Microbiology, 77(21): 7459-7468. doi: 10.1128/AEM.00267-11
[20]	XIA XIAOMIN, VIDYARATHNA N K, PALENIK B, et al, 2015. Comparison of the seasonal variations of Synechococcus assemblage structures in estuarine waters and coastal waters of Hong Kong[J]. Applied and Environmental Microbiology, 81(21): 7644-7655. doi: 10.1128/AEM.01895-15
[21]	XIA XIAOMIN, GUO WANG, TAN SHANGJIN, et al, 2017. Synechococcus assemblages across the salinity gradient in a salt wedge estuary[J]. Frontiers in Microbiology, 8: 1254, doi: 10.3389/fmicb.2017.01254. doi: 10.3389/fmicb.2017.01254
[22]	XIA XIAOMIN, LIU HONGBIN, CHOI D, et al, 2018. Variation of Synechococcus pigment genetic diversity along two turbidity gradients in the China Seas[J]. Microbial Ecology, 75(1): 10-21. doi: 10.1007/s00248-017-1021-z
[23]	XIA XIAOMIN, CHEUNG S, ENDO H, et al, 2019. Latitudinal and vertical variation of Synechococcus assemblage composition along 170° W transect from the South Pacific to the Arctic Ocean[J]. Microbial Ecology, 77(2): 333-342. doi: 10.1007/s00248-018-1308-8
[24]	XIA XIAOMIN, LEE P, CHEUNG S, et al, 2020. Discovery of euryhaline phycoerythrobilin-containing Synechococcus and its mechanisms for adaptation to estuarine environments[J]. mSystems, 5(6): e00842-20.
[25]	YAMANAKA G, GLAZER A N, 1981. Dynamic aspects of phycobilisome structure: modulation of phycocyanin content of Synechococcus phycobilisomes[J]. Archives of Microbiology, 130(1): 23-30. doi: 10.1007/BF00527067

成分		质量浓度/(g·L^-1)
常量元素	NaNO₃	7.5×10^-2
常量元素	NaH₂PO₄H₂O	5.0×10^-3
微量金属	FeCl₃·6H₂O Na₂EDTA₂·H₂O CuSO₄·5H₂O Na₂MoO₄·2H₂O ZnSO₄·7H₂O CoCl₂·6H₂O MnCl₄·4H₂O	3.15×10^-3 4.36×10^-3 9.8×10^-3 6.3×10^-3 2.2×10^-2 1.0×10^-2 1.8×10^-1
维生素	Thiamine HCl (vit. B1) Biotin (vit. H) Cyanocobalamin (vit. B12)	2.0×10^-4 1.0×10^-3 1.0×10^-3

盐度对广盐型聚球藻K1生长及转录组的影响*

Effects of salinity on the growth and transcriptome of euryhaline Synechococcus sp. K1*

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 25

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	林桂焕, 严幼芳, 刘颖. 印度尼西亚—澳大利亚海盆上层海洋层结变化及其影响因素[J]. 热带海洋学报, 2024, 43(4): 57-67.
[2]	吕泓柯, 巩远发, 王桂华. 印度洋海表盐度预报的线性马尔可夫模型及其改进办法[J]. 热带海洋学报, 2022, 41(6): 151-158.
[3]	董兰芳, 许明珠, 刘海娟, 曾梦清, 陈瑞芳, 李世才. 盐度对中国鲎幼鲎生长、蜕壳、Na⁺-K⁺-ATP酶活性、免疫指标和抗氧化能力的影响[J]. 热带海洋学报, 2022, 41(3): 156-163.
[4]	邱爽, 叶海军, 张玉红, 唐世林. 基于航次观测和再分析资料的南海海表二氧化碳分压反演及变化机制分析^*[J]. 热带海洋学报, 2022, 41(1): 106-116.
[5]	何子康, 王喜冬, 陈志强, 范开桂. 利用海洋温度剖面与海表盐度反演盐度剖面方法研究[J]. 热带海洋学报, 2021, 40(6): 41-51.
[6]	陆梦莹, 苏冒亮, 张俊彬. 盐度变化对金钱鱼感染嗜水气单胞菌后血清及肾脏免疫状态的影响[J]. 热带海洋学报, 2021, 40(3): 114-123.
[7]	潘肖兰, 刘惠茹, 许濛, 许瀚之, 张华, 何毛贤. 马氏珠母贝水通道蛋白基因AQP4 cDNA克隆和表达分析[J]. 热带海洋学报, 2020, 39(3): 66-75.
[8]	池建伟,曲堂栋,张莹,施平,杜岩. 热带东太平洋淡水池的季节变化 ^*[J]. 热带海洋学报, 2019, 38(5): 1-9.
[9]	王祥鹏,张玉红,王爱梅,赵玮,杜岩. 南海次表层盐度的低频变化及与太平洋年代际振荡的关系[J]. 热带海洋学报, 2019, 38(4): 1-9.
[10]	方祝骏, 智海, 林鹏飞, 魏翔. 利用热带太平洋关键区海表面盐度指标区分两类厄尔尼诺[J]. 热带海洋学报, 2019, 38(2): 32-42.
[11]	徐岩, 刘香全, 宋仁刚, 岑显荣, 郭双喜, 周生启. 扩散对流温盐台阶结构的数值模拟[J]. 热带海洋学报, 2019, 38(1): 11-18.
[12]	曹文浩, 严瑾, 丰美萍, 韩帅帅, 林明晴. 盐度对中国东南沿海两种常见藤壶幼虫发育的影响[J]. 热带海洋学报, 2018, 37(6): 85-91.
[13]	傅圆圆, 程旭华, 张玉红, 严幼芳, 杜岩. 近二十年南海表层海水的盐度淡化及其机制*[J]. 热带海洋学报, 2017, 36(4): 18-24.
[14]	孙启伟, 杜岩, 张玉红. 利用Argo和Aquarius卫星观测研究热带南印度洋海表盐度的季节变化[J]. 热带海洋学报, 2017, 36(4): 25-34.
[15]	王富轩, 肖述, 向志明, 喻子牛. 香港牡蛎 (Crassostrea hongkongensis) Commd1基因的分子克隆及其在盐度胁迫下的表达分析[J]. 热带海洋学报, 2017, 36(1): 48-55.