热带海洋学报 ›› 2010, Vol. 29 ›› Issue (4): 154-159.doi: 10.11978/j.issn.1009-5470.2010.04.154

• 海洋生物学 • 上一篇    下一篇

分子鉴定方法研究大亚湾水体弧菌种类变化

江晓1,2, 任春华1, 胡超群1, 罗鹏1, 陈偿1, 冯敬宾1,2   

  1. 1.中国科学院南海海洋研究所, 广东 广州 510301; 2.中国科学院研究生院, 北京100049
  • 收稿日期:2009-11-25 修回日期:2010-03-01 出版日期:2010-07-31 发布日期:2010-07-29
  • 通讯作者: 胡超群。
  • 作者简介:江晓(1983—), 湖北省黄冈市人, 硕士研究生, 主要从事弧菌检测与分型研究。
  • 基金资助:

    中国科学院知识创新工程重要方向项目(KZCX2-YW-Q07-03); 国家“973”课题(2006CB101803)

On dynamic change of Vibrio species in the Daya Bay using molecular identification method

JIANG Xiao1,2, REN Chun-hua1, HU Chao-qun1, LUO Peng1, CHEN Chang1, FENG Jing-bin1,2   

  1. 1. South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate University of CAS, Beijing 100049, China
  • Received:2009-11-25 Revised:2010-03-01 Online:2010-07-31 Published:2010-07-29
  • Contact: 胡超群。
  • About author:江晓(1983—), 湖北省黄冈市人, 硕士研究生, 主要从事弧菌检测与分型研究。
  • Supported by:

    中国科学院知识创新工程重要方向项目(KZCX2-YW-Q07-03); 国家“973”课题(2006CB101803)

摘要:

借助分子鉴定方法监测大亚湾水体中弧菌Vibrio种类季节性动态的变化规律。通过增菌培养、菌株分离, 在224份海水中共分离出弧菌368株, 并用分子生物学辅助生化鉴定方法鉴定弧菌菌株。结果表明,在大亚湾海域水体中鉴定的弧菌种类有溶藻弧菌Vibrio alginolyticus、副溶血弧菌V. parahaemolyticus、哈氏弧菌V. harveyi和创伤弧菌V. vulnificus, 没有检测到霍乱弧菌V. cholerae、拟态弧菌V. mimicus、河流弧菌V. fluvialis和霍利斯弧菌V. hollisae。溶藻弧菌和副溶血弧菌为优势菌群, 分别占弧菌总数的27.99%和21.74%。

关键词: 弧菌, 分子生物学鉴定, 种类组成, 大亚湾, 季节性分布

Abstract:

The authors investigate dynamic change regularity of Vibrio species in the Daya Bay using molecular identification method. Brine from different locations of the Daya Bay was cultured, purified and identified. A total of 368 Vibrio isolates were obtained from 224 seawater samples. Using molecular biology method following biochemical identification, they find Vibrio alginolyticus, V. parahaemolyticus, V. harveyi and V. vulnificus, but not V. cholerae, V. mimicus, V. vulnificus and V. hollisae in the brine from the Daya Bay. The results show that V. alginolyticus and V. parahaemolyticus are the dominant species, which occupy 27.99% and 21.74% of the overall Vibrio isolates, respectively.

Key words: Vibrio, molecular identification, species composition, the Daya Bay, seasonal distribution