环境DNA分析大亚湾夜光藻藻华对真核浮游生物群落的影响*

doi:10.11978/2022005

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (5): 121-132.doi: 10.11978/2022005CSTR: 32234.14.2022005

环境DNA分析大亚湾夜光藻藻华对真核浮游生物群落的影响^*

陈靖夫¹^,²^,³(), 钟瑜¹^,⁴, 王磊¹^,², 郭雨沛¹^,², 邱大俊¹^,²^,³()

1.热带海洋生物资源与生态重点实验室(中国科学院南海海洋研究所), 广东广州 510301
2.中国科学院大学, 北京 100049
3.南方海洋科学与工程广东省实验室(广州), 广东广州 511458
4.中国科学院大亚湾海洋生物综合实验站, 广东深圳 518121

收稿日期:2022-01-10 修回日期:2022-04-05 出版日期:2022-09-10 发布日期:2022-09-30
通讯作者: 邱大俊
作者简介:陈靖夫(1996—), 男, 硕士, 从事浮游植物研究。email: chenjingfu19@mails.ucas.ac.cn
基金资助:
国家自然科学基金(41776154);广州海洋实验室2019年度人才团队引进项目(GML2019ZD0405)

Noctiluca scintillans effects on eukaryotic plankton community structure using Environmental DNA analysis in Daya Bay*

CHEN Jingfu¹^,²^,³(), ZHONG Yu¹^,⁴, WANG Lei¹^,², GUO Yupei¹^,², QIU Dajun¹^,²^,³()

1. Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China
4. Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen 518121, China

Received:2022-01-10 Revised:2022-04-05 Online:2022-09-10 Published:2022-09-30
Contact: QIU Dajun
Supported by:
National Natural Science Foundation of China(41776154);Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(GML2019ZD0405)

摘要/Abstract

摘要：

夜光藻是全球广泛分布的藻华种之一, 其摄食作用对浮游生物的群落结构有着重要影响。利用环境DNA技术和Spearman相关性分析, 文章研究了2021年1月中旬大亚湾夜光藻高丰度站位浮游生物的群落结构与生物相关性。结果显示, 夜光藻高丰度站位的海水温盐条件为温度 14.4~18.3℃、盐度 32.52‰~33.14‰。夜光藻丰度较高的站位中, 卡顿藻科、海链藻科等浮游植物类群的相对丰度均值为2.41%和2.23%, 明显高于其他站位的0.75%和0.62%; 同时拟哲水蚤科与长腹剑水蚤科等类群的相对丰度均值为8.22%和4.46%, 明显低于其他站位的64.13%和11.12%。结合Spearman相关性分析表明, 夜光藻丰度增加可能导致其对拟哲水蚤科等类群摄食压力增加, 拟哲水蚤科类群丰度减少降低了卡顿藻科、海链藻科、原甲藻科、隐芽藻科等浮游植物类群的摄食压力, 使其生物丰度增加; 同时夜光藻摄食压力增加使得裸甲藻科、圆筛藻科等浮游植物丰度下降。因此, 夜光藻丰度增加影响了水域中真核浮游生物群落结构。综上结果表明, 环境DNA技术有助于认识夜光藻在浮游生物群落中的生态地位, 可用于浮游生物各类群之间的关系分析。

关键词: 大亚湾, 环境DNA, 夜光藻, 食性, 真核浮游生物, 群落结构

Abstract:

Noctiluca scintillans is a key blooms species and widely distributed throughout the world. Previous studies indicated that the plankton community was strongly influenced by the predation of N. scintillans. In this study, eukaryotic plankton community and biological correlation in the Daya Bay were analyzed using environmental DNA technology and Spearman correlate analysis in January 2021. The temperature and salinity of surface sea water were 14.4 ~ 18.3 ℃ and 32.52 ‰ ~ 33.14 ‰, respectively. In the stations with high N. scintillans abundance, the relative abundance of phytoplankton, such as Chattonellaceae and Thalassiosiraceae were 2.41 % and 2.23 %, which is significantly higher than the average values of 0.75 % and 0.62 % at other stations, respectively. While the relative abundances of Paracalanidae and Oithonidae were 8.22 % and 4.46 % at high N. scintillans abundance stations, which is significantly lower than the average value of 64.13 % and 11.12 % at other stations, respectively. These results indicated that N. scintillans reduced the top-down control of Paracalanidae on phytoplankton including Chattonellaceae, Thalassiosiraceae, Prorocentraceae and Geminigeraceae which led to an increase in the abundance of some plankton species in the blooms areas. Meanwhile, Gymnodiniaceae and Stephanopyxidaceae were fed by N. scintillans, which directly affected the community abundance of phytoplankton. Our analysis suggests that eDNA technology provides the methodological basis for understanding the ecological niche of N. scintillans, which would ultimately contribute to understanding the occurrence of blooms strategies.

Key words: Daya Bay, environmental DNA, Noctiluca scintillans, feeding habits, Eukaryotic plankton, community structure

中图分类号:

P735.124

陈靖夫, 钟瑜, 王磊, 郭雨沛, 邱大俊. 环境DNA分析大亚湾夜光藻藻华对真核浮游生物群落的影响^*[J]. 热带海洋学报, 2022, 41(5): 121-132.

CHEN Jingfu, ZHONG Yu, WANG Lei, GUO Yupei, QIU Dajun. Noctiluca scintillans effects on eukaryotic plankton community structure using Environmental DNA analysis in Daya Bay*[J]. Journal of Tropical Oceanography, 2022, 41(5): 121-132.

图/表 9

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站位	原始序列数	有效序列数	有效比	OTUs数
S1≥3µm	85852	83035	96.72	451
S1-pico	86846	82146	94.59	662
S2≥3µm	86414	83728	96.89	252
S2-pico	80261	76850	95.75	930
S3≥3µm	87818	84321	96.02	164
S3-pico	85984	82880	96.39	464
S4≥3µm	82743	79504	96.09	340
S4-pico	85679	82797	96.64	474
S5≥3µm	87153	84519	96.98	212
S5-pico	87939	84033	95.56	509
S6≥3µm	83419	80163	96.10	251
S6-pico	85651	82202	95.97	582
S7≥3µm	86571	83124	96.02	398
S7-pico	82757	80002	96.67	253
站位	原始序列数	有效序列数	有效比	OTUs数
S8≥3µm	80444	77315	96.11	397
S8-pico	81353	76092	93.53	799
S9≥3µm	80922	77302	95.53	638
S9-pico	81500	78682	96.54	345
S10≥3µm.	87029	83148	95.54	618
S10-pico	82076	77528	94.46	619
S11≥3µm	82660	79646	96.35	223
S11-pico	85237	81181	95.24	527
S12≥3µm	85806	82684	96.36	212
S12-pico	86122	81857	95.05	624

环境DNA分析大亚湾夜光藻藻华对真核浮游生物群落的影响^*

Noctiluca scintillans effects on eukaryotic plankton community structure using Environmental DNA analysis in Daya Bay*

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