热带海洋学报 ›› 2012, Vol. 31 ›› Issue (2): 96-101.doi: 10.11978/j.issn.1009-5470.2012.02.013

• 海洋生物学 • 上一篇    下一篇

生长激素与胰岛素样生长因子 - Ⅰ对马氏珠母贝壳生长形成相关基因表达的影响

张立娟 1,2 , 何毛贤 1   

  1. 1. 中国科学院南海海洋研究所 , 中国科学院海洋生物资源可持续利用重点实验室 , 广东 广州 510301; 2 .中国科学院研究生院 , 北京 100049
  • 收稿日期:2010-12-02 修回日期:2011-01-24 出版日期:2012-06-05 发布日期:2012-06-05
  • 作者简介: 张立娟 (1986 — ), 女 , 黑龙江省绥化市人 , 硕士研究生 , 主要从事海洋生物研究。
  • 基金资助:
    国家自然科学基金 - 广东联合基金重点项目 (U0831001); 中国科学院知识创新工程重要方向项目 (KZCX2-EW-Q21); 广东省产学研项目 (2010B090300075); 广东省科技计划项目 (2008A020100004)

Effects of growth hormone and insulin-like growth factor- Ⅰ on shell growth related gene expression in Pinctada fucata Gould

ZHANG Li-juan1, 2, HE Mao-xian2   

  1. 1. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology , Chinese Academy of Sciences , Guangzhou 510301, China 2. Graduate University of Chinese Academy of Sciences , Beijing 100049, China
  • Received:2010-12-02 Revised:2011-01-24 Online:2012-06-05 Published:2012-06-05

摘要: 研究了外源生长激素与胰岛素样生长因子 - Ⅰ处理对马氏珠母贝 Pinctada fucata Gould 胰岛素相关多肽受体基因 ( irr) 和 5 种壳基质蛋白基因 ( nacrein efcbp n 19 、 aspein accbp ) 表达水平的影响。结果表明 , 激素处理显著提高了 irr 基因的表达水平 ( P<0.05), 这与其在软体动物中作为胰岛素样生长因子受体的作用相一致。 nacrein 基因的表达水平在激素处理组也有显著升高 ( P<0.05), 表明生长激素与胰岛素样生长因子 - Ⅰ 两种外源激素都增强了马氏珠母贝的生长代谢水平|与对照组相比 , n19 、 aspein accbp 3 个基因的表达水平在激素处理组均下调 ( P<0.05), 说明这 3 个基因的表达受到激素调节通路的抑制作用。此外 , 研究发现 aspein accbp 两个基因的表达在各个实验样本中具有极高的相关性 , 说明这两个基因在激素通路中可能受到同一个上游因子调控。 efcbp 基 因表达水平在激素处理组与对照组之间表达稳定 , 各样本之间无显著性的变化 ( P >0.05 ) 。

关键词: 生长激素, 胰岛素样生长因子-Ⅰ, 胰岛素相关多肽受体, 壳基质蛋白, 基因表达, 马氏珠母贝

Abstract: This study investigated the expression levels of insulin-related peptide receptor gene ( irr) and several shell matrix protein genes ( nacrein, efcbp, n19, aspein, and accbp) in human recombinant growth hormone injection and insulin-like growth factor injection groups compared to the reference group in pearl oyster Pinctada fucata Gould. The expression level of irr was increased by hormone treatments ( P<0.05), which is consistent with its role as insulin-like growth factor receptor in mollusk. Higher transcripts of nacrein indicated higher growth metabolism levels in the pearl oysters of the experimental groups; compared to the reference group, the expression levels of n19, aspein and accbp decreased after growth hormone (GH) and insulin-like growth factor- Ⅰ (IGF- Ⅰ ) treatments ( P<0.05), suggesting they were suppressed by the GH and IGF- Ⅰ pathways. In addition, the expression levels of aspein and accbp were found to be highly correlated; we infer that these two genes may be regulated by a single upstream factor. The expression level of efcbp showed a relatively steady expression in all oysters, and no significant differences were found among these groups ( P>0.05).

Key words: growth hormone, insulin-like growth factor- Ⅰ, insulin-related peptide receptor, shell matrix protein, gene expression, Pinctada fucata Gould

中图分类号: 

  • Q959.215