生长激素与胰岛素样生长因子 - Ⅰ对马氏珠母贝壳生长形成相关基因表达的影响

doi:10.11978/j.issn.1009-5470.2012.02.013

热带海洋学报 ›› 2012, Vol. 31 ›› Issue (2): 96-101.doi: 10.11978/j.issn.1009-5470.2012.02.013cstr: 32234.14.j.issn.1009-5470.2012.02.013

生长激素与胰岛素样生长因子 - Ⅰ对马氏珠母贝壳生长形成相关基因表达的影响

张立娟 ^1,2 , 何毛贤 ¹

1. 中国科学院南海海洋研究所 , 中国科学院海洋生物资源可持续利用重点实验室 , 广东广州 510301; 2 ．中国科学院研究生院 , 北京 100049

收稿日期:2010-12-02 修回日期:2011-01-24 出版日期:2012-06-05 发布日期:2012-06-05
作者简介: 张立娟 (1986 — ), 女 , 黑龙江省绥化市人 , 硕士研究生 , 主要从事海洋生物研究。
基金资助:
国家自然科学基金 - 广东联合基金重点项目 (U0831001); 中国科学院知识创新工程重要方向项目 (KZCX2-EW-Q21); 广东省产学研项目 (2010B090300075); 广东省科技计划项目 (2008A020100004)

Effects of growth hormone and insulin-like growth factor- Ⅰ on shell growth related gene expression in Pinctada fucata Gould

ZHANG Li-juan¹, ², HE Mao-xian²

1. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology , Chinese Academy of Sciences , Guangzhou 510301, China 2. Graduate University of Chinese Academy of Sciences , Beijing 100049, China

Received:2010-12-02 Revised:2011-01-24 Online:2012-06-05 Published:2012-06-05

摘要/Abstract

摘要： 研究了外源生长激素与胰岛素样生长因子 - Ⅰ处理对马氏珠母贝 Pinctada fucata Gould 胰岛素相关多肽受体基因 ( irr) 和 5 种壳基质蛋白基因 ( nacrein 、 efcbp 、 n 19 、 aspein 及 accbp ) 表达水平的影响。结果表明 , 激素处理显著提高了 irr 基因的表达水平 ( P<0.05), 这与其在软体动物中作为胰岛素样生长因子受体的作用相一致。 nacrein 基因的表达水平在激素处理组也有显著升高 ( P<0.05), 表明生长激素与胰岛素样生长因子 - Ⅰ 两种外源激素都增强了马氏珠母贝的生长代谢水平|与对照组相比 , n19 、 aspein 与 accbp 3 个基因的表达水平在激素处理组均下调 ( P<0.05), 说明这 3 个基因的表达受到激素调节通路的抑制作用。此外 , 研究发现 aspein 与 accbp 两个基因的表达在各个实验样本中具有极高的相关性 , 说明这两个基因在激素通路中可能受到同一个上游因子调控。 efcbp 基因表达水平在激素处理组与对照组之间表达稳定 , 各样本之间无显著性的变化 ( P >0.05 ) 。

关键词: 生长激素, 胰岛素样生长因子-Ⅰ, 胰岛素相关多肽受体, 壳基质蛋白, 基因表达, 马氏珠母贝

Abstract: This study investigated the expression levels of insulin-related peptide receptor gene ( irr) and several shell matrix protein genes ( nacrein, efcbp, n19, aspein, and accbp) in human recombinant growth hormone injection and insulin-like growth factor injection groups compared to the reference group in pearl oyster Pinctada fucata Gould. The expression level of irr was increased by hormone treatments ( P<0.05), which is consistent with its role as insulin-like growth factor receptor in mollusk. Higher transcripts of nacrein indicated higher growth metabolism levels in the pearl oysters of the experimental groups; compared to the reference group, the expression levels of n19, aspein and accbp decreased after growth hormone (GH) and insulin-like growth factor- Ⅰ (IGF- Ⅰ ) treatments ( P<0.05), suggesting they were suppressed by the GH and IGF- Ⅰ pathways. In addition, the expression levels of aspein and accbp were found to be highly correlated; we infer that these two genes may be regulated by a single upstream factor. The expression level of efcbp showed a relatively steady expression in all oysters, and no significant differences were found among these groups ( P>0.05).

Key words: growth hormone, insulin-like growth factor- Ⅰ, insulin-related peptide receptor, shell matrix protein, gene expression, Pinctada fucata Gould

中图分类号:

Q959.215

张立娟,何毛贤. 生长激素与胰岛素样生长因子 - Ⅰ对马氏珠母贝壳生长形成相关基因表达的影响[J]. 热带海洋学报, 2012, 31(2): 96-101.

ZHANG Li-juan,HE Mao-xian. Effects of growth hormone and insulin-like growth factor- Ⅰ on shell growth related gene expression in Pinctada fucata Gould[J]. Journal of Tropical Oceanography, 2012, 31(2): 96-101.

参考文献

BLUM W F, ALBERTSSON K W, ROSBERG S, et al. Serum levels of insulin-like growth factor I (IGF-Ⅰ) and IGF binding protein 3 reflect spontaneous growth hormone secretion[J]. J Clin Endocrinol Metab, 1993, 76(6): 1610-1616. MANDEL S, MORELAND E, NICHOLS V, et al. Changes in insulin-like growth factor-Ⅰ (IGF-Ⅰ), IGF-binding protein-3, growth hormone (GH)-binding protein, erythrocyte IGF-Ⅰ receptors, and growth rate during GH treatment[J]. J Clin Endocrinol Metab, 1995, 80(1): 190-194. GOMOT A, GOMOT L. Neurohormonal control of body and shell growth of the snail Helix[M]//ALLEMAND D, CUIF J. Monaco: Bull de l'Institut Oceanogr, 1995: 141-149. SALEUDDIN A S M, KHAN H R, SEVALA M, et al. Hormonal control of confirmed shell growth in the snail Helisoma duryi (Mollusca: Gastropoda)[M]//SUGA S, NAKAHARA H. Mechanisms and phylogeny of mineralization in biological systems. Tokyo: Springer, 1991: 149-169. GIARD W, LEBEL J M, BOUCAUD E C, et al. Effects of vertebrate growth factors on digestive gland cells from the mollusc Pecten maximus L.: An in vitro study[J]. J Comp Physiol B, 1998, 168(2): 81-86. GRICOURT L, BONNEC G, BOUJARD D, et al. Insulin-like system and growth regulation in the Pacific oyster Crassostrea gigas: hrIGF-1 effect on protein synthesis of mantle edge cells and expression of an homologous insulin receptor-related receptor[J]. Gen Comp Endocr, 2003, 134(1): 44-56. 金启增, 黎辉, 何慧. 珍珠生长激素在合浦珠母贝育珠中的作用[J]. 热带海洋, 1998, 17(4): 44-50. KELLNER C K, MIALHE E, MATHIEU M. Identification of insulin-like peptides in cerebral ganglia neurosecretory cells of the mussel Mythus edulis[J]. Tissue Cell, 1994, 26(6): 891-899. HAMANO K, AWAJI M, USUKI H. cDNA structure of an insulin-related peptide in the Pacific oyster and seasonal changes in the gene expression[J]. J Endocrinol, 2005, 187(1): 55-67. WILT F H, KILLIAN C E, LIVINGSTON B T. Development of calcareous skeletal elements in invertebrates[J]. Differentiation, 2003, 71(4-5): 237-250. MARIN F, LUQUET G, MARIE B, et al. Molluscan shell proteins: primary structure, origin, and evolution[J]. Curr Top Dev Biol, 2008, 80: 209-76. TSUKAMOTO D, SARASHINA I, ENDO K. Structure and expression of an unusually acidic matrix protein of pearl oyster shells[J]. Biochem Biophys Res Commun, 2004, 320(4): 1175-1180. SUZUKI M, MURAYAMA E, INOUE H, et al. Characterization of Prismalin-14, a novel matrix protein from the prismatic layer of the Japanese pearl oyster (Pinctada fucata)[J]. Biochem J, 2004, 382(1): 205-213. SUDO S, FUJIKAWA T, NAGAKURA T, et al. Structures of mollusc shell framework proteins[J]. Nature, 1997, 387: 563-564. SAMATA T, HAYASHI N, KONO M, et al. A new matrix protein family related to the nacreous layer formation of Pinctada fucata[J]. FEBS Lett, 1999, 462(1-2): 225-229. YANO M, NAGAI K, MORIMOTO K, et al. A novel nacre protein N19 in the pearl oyster Pinctada fucata[J]. Biochem Biophys Res Commun, 2007, 362(1): 158-163. MIYAMOTO H, MIYASHITA T, OKUSHIMA M, et al. A carbonic anhydrase from the nacreous layer in oyster pearls[J]. Proc Natl Acad Sci, 1996, 93(18): 9657-9660. HUANG J, ZHANG C, MA Z J, et al. A novel extracellular EF-hand protein involved in the shell formation of pearl oyster[J]. Biochim Biophys Acta, 2007, 1770(7): 1037-1044. MA Z J, HUANG J, SUN J, et al. A novel extrapallial fluid protein controls the morphology of nacre lamellae in the pearl oyster, Pinctada fucata[J]. J Biol Chem, 2007, 282(32): 23253-23263. ZHANG C, ZHANG R Q. Matrix proteins in the outer shells of molluscs[J]. Mar Biotechnol, 2006, 8(6): 572-586. LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2???CT method[J]. Methods, 2001, 25(4): 402-408. 邓利, 张为民, 林浩然. 生长激素受体的研究进展[J]. 动物学研究, 2001, 22(3): 226-230. LIU J L, LEROITH D. Insulin-like growth factor I is essential for postnatal growth in response to growth hormone[J]. Endocrinology, 1999, 140(11): 5178-5184. 许光武, 俞茂华. 胰岛素样生长因子[J]. 中华内分泌代谢杂志, 2000, 16(1): 52-55. LARDANS V, COPPIN J F, VICOGNE J, et al. Characterization of an insulin receptor-related receptor in Biomphalaria glabrata embryonic cells[J]. Biochim Biophys Acta, 2001, 1510(1-2): 321-329. NISHIDA Y, HATA M, NISHIZUKA Y, et al. Cloning of a drosophila cDNA encoding a polypeptide similar to the human insulin receptor precursor[J]. Biochem Biophys Res Commun, 1986, 141(2): 474-481. KIMURA K D, TISSENBAUM H A, LIU Y, et al. daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans[J]. Science, 1997, 227(5328): 942-946. GOMOT A, GOMOT L, MARCHAND C-R et al. Immunocytochemical localization of insulin-related peptide(s) in the central nervous system of the snail Helix aspersa müller: involvement in growth control[J]. Cell Mol Neurobiol, 1992, 12(1): 21-32. GRAF R, NEUENSCHWANDER S, BROWN M R, et al. Insulin-mediated secretion of ecdysteroids from mosquito ovaries and molecular cloning of the insulin receptor homologue from ovaries of bloodfed Aedes aegypti[J]. Insect Mol Biol, 1997, 6(2): 151-163. SONETTI D, VAN HEUMEN W R A, ROUBOS E W. Light- and electron-microscopic immunocytochemistry of a molluscan insulin-related peptide in the central nervous system of Planorbarius corneus[J]. Cell Tissue Res, 1992, 267(3): 473-481. GRICOURT L, MATHIEU M, KELLNER K. An insulin-like system involved in the control of Pacific oyster Crassostrea gigas reproduction: hrIGF-1 effect on germinal cell proliferation and maturation associated with expression of an homologous insulin receptor-related receptor[J]. Aquaculture, 2006, 251(1): 85-98. MIYAMOTO H, MIYOSHI F, KOHNO J. The carbonic anhydrase domain protein Nacrein is expressed in the epithelial cells of the mantle and acts as a negative regulator in the calcification in the mollusc Pinctada fucata[J]. Zool Sci, 2005, 22(3): 311-315. GONG N P, LI Q, HUANG J, et al. Culture of outer epithelial cells from mantle tissue to study shell matrix protein secretion for biomineralization[J]. Cell Tissue Res, 2008, 333(3): 493-501. ABDRABA A M, Saleuddin A S. Protein synthesis in vitro by mantle tissue of the land snail Otala lactea: possible insulin-like peptide function [J]. Can J Zool, 2000, 78(9): 1527-1535.

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生长激素与胰岛素样生长因子 - Ⅰ对马氏珠母贝壳生长形成相关基因表达的影响

Effects of growth hormone and insulin-like growth factor- Ⅰ on shell growth related gene expression in Pinctada fucata Gould

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