CO2升高对风信子鹿角珊瑚(Acropora hyacinthus)钙化速率和基因表达的影响*

doi:10.11978/2022182

热带海洋学报 ›› 2024, Vol. 43 ›› Issue (3): 40-48.doi: 10.11978/2022182CSTR: 32234.14.2022182

CO₂升高对风信子鹿角珊瑚(Acropora hyacinthus)钙化速率和基因表达的影响^*

袁翔城¹^,²^,³^,⁴^,⁵(), 梁宇娴¹^,²^,³^,⁶, 宋严¹^,²^,³^,⁶, 俞晓磊¹^,²^,³^,⁶, 黄晖¹^,²^,³^,⁴^,⁵, 周伟华¹^,²^,³^,⁴^,⁵()

1.中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东省应用海洋生物学重点实验室, 广东广州 510301
2.三亚海洋生态环境工程研究院, 三亚海洋科学综合(联合)实验室, 海南省热带海洋生物技术重点实验室, 海南三亚 572000
3.中国科学院海南热带海洋生物实验站, 海南三亚海洋生态系统国家野外科学观测研究站, 海南三亚 572000
4.中国科学院南海生态环境工程创新研究院, 广东广州 510301
5.南方海洋科学与工程广东省实验室(广州), 广东广州 511458
6.中国科学院大学, 北京 100049

收稿日期:2022-08-23 修回日期:2022-12-22 出版日期:2024-05-10 发布日期:2022-12-15
作者简介:
袁翔城(1977—), 男, 江西省赣州市人, 博士, 研究员, 从事珊瑚生物学和珊瑚礁生态学研究
*感谢珊瑚生物学与珊瑚礁生态学学科组全体人员对本论文的完成提供的帮助。
基金资助:
国家重点研发项目(2021YFF0502800)

Effects of ocean acidification on the calcification and gene expression in coral Acropora hyacinthus^*

YUAN Xiangcheng¹^,²^,³^,⁴^,⁵(), LIANG Yuxian¹^,²^,³^,⁶, SONG Yan¹^,²^,³^,⁶, YU Xiaolei¹^,²^,³^,⁶, HUANG Hui¹^,²^,³^,⁴^,⁵, ZHOU Weihua¹^,²^,³^,⁴^,⁵()

1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. CAS-HKUST Sanya Joint Laboratory of Marine Science Research, Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Sanya Marine Ecological Environment Engineering Research Institute, Sanya 572000, China
3. Sanya Institute of Marine Ecological Environment Engineering, Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya 572000, China
4. South China Sea Ecological Environment Engineering Innovation Research Institute, Chinese Academy of Sciences, Guangzhou 510301, China
5. Guangdong Provincial Laboratory of Southern Marine Science and Engineering (Guangzhou), Guangzhou 511458, China
6. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2022-08-23 Revised:2022-12-22 Online:2024-05-10 Published:2022-12-15
Supported by:
National Key Research and Development Program of China(2021YFF0502800)

摘要/Abstract

摘要：

本文以风信子鹿角珊瑚(Acropora hyacinthus)为研究对象, 以三亚珊瑚礁海域自然海水培养的珊瑚样本为对照组, 模拟本世纪末海水酸化状况, 并开展珊瑚模拟培养试验。以此探究海水酸化对A. hyacinthus钙化和基因表达的影响。在酸化胁迫下, A. hyacinthus净钙化速率显著下降, 基因表达受到显著影响, 其中有机物运输、阴离子运输、化学应激等通路上调, 动物器官发育、脂质转运、细胞生物合成、细胞表面受体信号等通路下调。与钙化相关的基因中, 碳酸酐酶和溶质运载蛋白下调, 但是Ca²⁺-ATP酶和钙离子运输相关的基因上调。结果表明, 高CO₂影响了A. hyacinthus的钙化和多个生物过程的基因表达, 海水酸化可能通过抑制珊瑚碳酸酐酶等基因表达, 减少$\mathrm{HCO}_{3}^{-}$运输, 最终降低钙化速率。

关键词: 海水酸化, 风信子鹿角珊瑚, 基因表达, 钙化

Abstract:

We studied the responses of coral Acropora hyacinthus to the increase of CO₂, with a simulated experiment in which corals were cultured in natural seawater as the control group with pCO₂ of 565 μatm and in high CO₂ treatment with pCO₂ of 1135 μatm. The effects of seawater acidification on the calcification and gene expression were investigated. The results showed that under acidification stress, the calcification rate of A. hyacinthus significantly decreased, and the gene expression was greatly affected. As the pathways of organic matter transport, anion transport, chemical stress were up-regulated, and animal organ development, lipid transport, cell biosynthesis, cell surface receptor signal and other pathways were down-regulated. Among the calcification related genes, carbonic anhydrase was down-regulated, and Ca²⁺-ATPase and calcium ion transport related genes were up-regulated. This experiment showed that high CO₂ significantly affected the calcification and gene expression of A. hyacinthus, in which the decrease of calcification was mainly due to the down-regulation of carbonic anhydrase resulting in the decrease of $\mathrm{HCO}_{3}^{-}$.

Key words: seawater acidification, Acropora hyacinthus, gene expression, calcification

袁翔城, 梁宇娴, 宋严, 俞晓磊, 黄晖, 周伟华. CO₂升高对风信子鹿角珊瑚(Acropora hyacinthus)钙化速率和基因表达的影响^*[J]. 热带海洋学报, 2024, 43(3): 40-48.

YUAN Xiangcheng, LIANG Yuxian, SONG Yan, YU Xiaolei, HUANG Hui, ZHOU Weihua. Effects of ocean acidification on the calcification and gene expression in coral Acropora hyacinthus^*[J]. Journal of Tropical Oceanography, 2024, 43(3): 40-48.

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CO₂升高对风信子鹿角珊瑚(Acropora hyacinthus)钙化速率和基因表达的影响^*

Effects of ocean acidification on the calcification and gene expression in coral Acropora hyacinthus^*

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