形态学和SNP标记分析马氏珠母贝杂交子代及其亲本群体的遗传结构

doi:10.11978/2019012

热带海洋学报 ›› 2019, Vol. 38 ›› Issue (6): 80-89.doi: 10.11978/2019012CSTR: 32234.14.2019012

形态学和SNP标记分析马氏珠母贝杂交子代及其亲本群体的遗传结构

黄景^1,^2,³, 潘肖兰^1,^2,³, 许濛^1,^2,³, 刘文广^1,³, 张华^1,³, 何毛贤^1,³()

^1. 中国科学院南海海洋研究所, 中国科学院热带海洋生物资源与生态重点实验室, 广东广州 510301
^2. 中国科学院大学, 北京 100049
^3. 中国科学院南海海洋研究所, 广东省应用海洋生物学重点实验室, 广东广州 510301

收稿日期:2019-01-21 修回日期:2019-02-27 出版日期:2019-11-20 发布日期:2019-11-26
通讯作者: 何毛贤
作者简介:黄景(1993—), 男, 湖北省黄石市人, 硕士研究生, 研究方向为马氏珠母贝分子遗传育种。E-mail: 1152524871@qq.com
基金资助:
国家贝类产业技术体系建设专项(CARS-49)

Morphological and SNP markers for analysis of genetic structure of hybrid progeny and their parental populations of Pinctada fucata martensii

HUANG Jing^1,^2,³, PAN Xiaolan^1,^2,³, XU Meng^1,^2,³, LIU Wenguang^1,³, ZHANG Hua^1,³, HE Maoxian^1,³()

^1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
^2. University of Chinese Academy of Sciences, Beijing 100049, China
^3. Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

Received:2019-01-21 Revised:2019-02-27 Online:2019-11-20 Published:2019-11-26
Contact: Maoxian HE
Supported by:
Modern Agro-industry Technology Research System(CARS-49)

摘要/Abstract

摘要：

马氏珠母贝是重要的海水养殖贝类, 为了探究繁育亲本及其子代的遗传结构和关系, 本研究运用多元性形态学和SNP标记对马氏珠母贝母本深圳群体、父本海南群体和其杂交子代F1的外部形态和分子遗传结构进行分析。结果发现, 3个群体的综合判别率为72%, F1和母本群体的形态差异最小, 父本群体与母本、F1的形态差异较大。采用HRM法 (high resolution melting) 应用4个SNP位点对这3个群体进行分型, 3个群体的平均观测杂合度H_o和期望杂合度H_e分别为0.2110~0.2879和0.3317~0.4685, F1的杂合度高于两个亲本; 平均多态信息含量PIC值为0.2643~0.3556, 呈现中等程度遗传多样性。F1与母本之间的基因流N_m最大(7.7701), 遗传距离最小(0.0546), 亲缘关系最近; 两个亲本之间的N_m最小(1.9662), 遗传距离最大(0.1759)。rs8位点可以判别两个亲本群体, 可作为特异性的标记。该结果可以为马氏珠母贝群体遗传结构鉴别、育种群体管理提供指导。

关键词: 马氏珠母贝, 遗传学, 遗传结构, 形态学, SNP

Abstract:

The pearl oyster, Pinctada fucata martensii, is a primary marine bivalve species. To study the genetic structure and relationship of cultured parents and their hybrid generation, external morphology and genetic structure were assessed in the three populations of P. fucata martensii from Shenzhen population (female), Hainan population (male) and their hybrid generation F1, based on morphological multivariate analysis and SNP markers. The results showed that the average discriminant accuracy was 72%, and the morphological characteristics of F1 and female were similar, while male was the most different from the other two populations. Four SNP markers of the three populations were genotyped by the HRM (high resolution melting) method. The results showed that the average observed (H_o) and expected (H_e) heterozygosity were 0.2110 to 0.2879 and 0.3317 to 0.4685, respectively; and the heterozygosity of F1 was higher than that of the parent populations. The average polymorphism information content (PIC) values of the three populations were in the range of 0.2643 to 0.3556, which showed moderate genetic diversity. Gene flow (N_m) between F1 and female was the largest (7.7701), with minimum genetic distance being 0.0546; thus, their genetic relationships were similar. Gene flow (N_m) between the parent populations was minimum (1.9662), with largest genetic distance being 0.1759. Finally, we found that rs8 marker can be used as a specific marker to discriminate parent populations. These results should assist the discriminate of genetic structure and management of selective breeding of P. fucata martensii.

Key words: Pinctada fucata martensii, genetics, genetic structure, morphology, SNP

黄景, 潘肖兰, 许濛, 刘文广, 张华, 何毛贤. 形态学和SNP标记分析马氏珠母贝杂交子代及其亲本群体的遗传结构[J]. 热带海洋学报, 2019, 38(6): 80-89.

HUANG Jing, PAN Xiaolan, XU Meng, LIU Wenguang, ZHANG Hua, HE Maoxian. Morphological and SNP markers for analysis of genetic structure of hybrid progeny and their parental populations of Pinctada fucata martensii[J]. Journal of Tropical Oceanography, 2019, 38(6): 80-89.

图/表 10

表1

表2

表3

表4

图1

表5

表6

图2

表7

图3

参考文献 57

1	白牡丹, 王彩虹, 殷豪 , 等, 2012. 苹果不同HRM反应体系分析效果评价[J]. 分子植物育种, 10(1):115-120.
	BAI MUDAN, WANG CAIHONG, YIN HAO , et al, 2012. Evaluation of different reaction systems for HRM analysis in apple[J]. Molecular Plant Breeding, 10(1):115-120 (in Chinese with English abstract).
2	陈亮, 赵柏淞, 李玲 , 等, 2014. 人工诱导栉孔扇贝雌核发育胚胎的SNP标记分析[J]. 中国海洋大学学报, 44(2):48-52.
	CHEN LIANG, ZHAO BOSONG, LI LING , et al, 2014. Genotyping artificially induced gynogenetic embryos of Zhikong scallop (Chlamys farreri) with SNP markers[J]. Periodical of Ocean University of China, 44(2):48-52 (in Chinese with English abstract).
3	陈蓉, 刘建勇, 唐连俊 , 等, 2009. 中国5个海区不同群体毛蚶形态差异分析[J]. 海洋科学, 33(7):64-69.
	CHEN RONG, LIU JIANYONG, TANG LIANJUN , et al, 2009. Morphological variations analysis of five different populations of Scapharca subcrenata in China[J]. Marine Sciences, 33(7):64-69 (in Chinese with English abstract).
4	董志国, 李家乐, 郑汉丰 , 2008. 三角帆蚌3个地理种群自交与杂交F1代的形态差异分析[J]. 大连水产学院学报, 23(2):92-97.
	DONG ZHIGUO, LI JIALE, ZHENG HANFENG , 2008. Morphological variability in original parents and their reciprocal hybrids F1 of fresh water mussel Hyriopsis cumingii from three geographical populations in China[J]. Journal of Dalian Fisheries University, 23(2):92-97 (in Chinese with English abstract).
5	杜晓东, 李广丽, 刘志刚 , 等, 2002. 合浦珠母贝2个野生种群的遗传多样性[J]. 中国水产科学, 9(2):100-105.
	DU XIAODONG, LI GUANGLI, LIU ZHIGANG , et al, 2002. Genetic diversity of two wild populations in Pinctada martensii[J]. Journal of Fishery Sciences of China, 9(2):100-105 (in Chinese with English abstract).
6	谷龙春, 李金碧, 喻达辉 , 等, 2010. 合浦珠母贝双列杂交家系的建立与遗传分析[J]. 水产学报, 34(1):26-31.
	GU LONGCHUN, LI JINBI, YU DAHUI , et al, 2010. Establishment and genetic analysis of complete diallel cross families of pearl oyster (Pinctada fucata)[J]. Journal of Fisheries of China, 34(1):26-31 (in Chinese with English abstract).
7	顾志峰, 王嫣, 石耀华 , 等, 2009. 马氏珠母贝两个不同地理种群的形态性状和贝壳珍珠质颜色比较分析[J]. 渔业科学进展, 30(1):79-86.
	GU ZHIFENG, WANG YAN, SHI YAOHUA , et al, 2009. Comparison of morphometrics and shell nacre colour between two geographical populations of pearl oyster Pinctada martensii (Dunker)[J]. Marine Fisheries Research, 30(1):79-86 (in Chinese with English abstract).
8	侯战辉, 王嫣, 石耀华 , 等, 2008. 马氏珠母贝(Pinctada martensii)2个不同地理种群遗传变异的EST-SSR分析[J]. 海洋与湖沼, 39(2):178-183. doi: 10.11693/hyhz20080229029
	HOU ZHANHUI, WANG YAN, SHI YAOHUA , et al, 2008. EST-SSR analysis of genetic variation between two geographical populations of pearl oyster, Pinctada martensii (Dunker)[J]. Oceanologia et Limnologia Sinica, 39(2):178-183 (in Chinese with English abstract). doi: 10.11693/hyhz20080229029
9	胡笑蓉, 毛雄英, 王卫华 , 等, 2012. 一种基于HRM技术的快速DNA甲基化检测方法[J]. 中国卫生检验杂志, 22(6):1336-1338.
	HU XIAORONG, MAO XIONGYING, WANG WEIHUA , et al, 2012. A novel and reliable method of DNA methylation analysis based on HRM technology[J]. Chinese Journal of Health Laboratory Technology, 22(6):1336-1338 (in Chinese with English abstract).
10	姜因萍, 何毛贤, 林岳光 , 2007. 马氏珠母贝群体内遗传多样性的ISSR分析[J]. 海洋通报, 26(5):62-66.
	JIANG YINPING, HE MAOXIAN, LIN YUEGUANG , 2007. Genetic diversity of a cultured population on Pinctada Martensii Dunker by ISSR marker[J]. Marine Science Bulletin, 26(5):62-66 (in Chinese with English abstract).
11	金玉琳 , 2014. 长牡蛎SNP标记开发及其在家系分析和物种鉴定中的应用[D]. 青岛: 中国海洋大学: 32-38.
	JIN YULIN , 2014. Development and characterization of SNP markers and their application in family analysis and species identification for Pancific oyster Crassostrea gigas[D]. Qingdao: Ocean University of China: 32-38 (in Chinese with English abstract).
12	李纪勤 , 2012. 栉孔扇贝(Chlamys farreri)EST-SNP的开发及其应用[D]. 青岛: 中国海洋大学: 53-54.
	LI JIQIN , 2012. Development, characterization and application of EST-SNP markers in Zhikong scallop (Chlamys farreri)[D]. Qingdao: Ocean University of China: 53-54 (in Chinese with English abstract).
13	李梅, 吕伟朋, 韦鸿 , 等, 2012. HRM方法检测非小细胞肺癌患者胸水标本癌细胞基因突变的临床意义[J]. 大连医科大学学报, 34(4):321-323, 347.
	LI MEI, LÜ WEIPENG, WEI HONG , et al, 2012. Feasibility of HRM to detect gene mutations in hydrothorax of patients with non -small cell lung cancer[J]. Journal of Dalian Medical University, 34(4):321-323, 347 (in Chinese with English abstract).
14	李太武, 孙修勤, 刘艳 , 等, 2002. 中日栉孔扇贝杂交子一代群体的遗传变异[J]. 高技术通讯, 12(6):101-105, 100.
	LI TAIWU, SUN XIUQIN, LIU YAN , et al, 2002. Allozyme variation of two subspecies of Chlamys farreri and their reciprocal hybrids[J]. High Technology Letters, 12(6):101-105, 100 (in Chinese with English abstract).
15	李耀国, 刘文广, 林坚士 , 等, 2016. 马氏珠母贝SNP标记开发及家系遗传多态性分析[J]. 海洋通报, 35(1):96-102.
	LI YAOGUO, LIU WENGUANG, LIN JIANSHI , et al, 2016. Development of SNP markers in Pinctada fucata and its application for family genetic analysis[J]. Marine Science Bulletin, 35(1):96-102 (in Chinese with English abstract).
16	刘泽浩, 李捷, 杨学玲 , 等, 2010 中国北方沿海潮间带常见镜蛤的种类[J]. 海洋科学, 34(12):30-35.
	LIU ZEHAO, LI JIE, YANG XUELING , et al, 2010. Species of common Dosinia from intertidal zones of northern China[J]. Marine Sciences, 34(12):30-35 (in Chinese with English abstract).
17	罗会, 刘宝锁, 黎火金 , 等, 2013. 合浦珠母贝不同地理种群的形态差异和判别分析[J]. 广东农业科学, 40(12):171-174.
	LUO HUI, LIU BAOSUO, LI HUOJIN , et al, 2013. Morphological variations and discriminant analysis on four populations of Pinctada fucata[J]. Guangdong Agricultural Sciences, 40(12):171-174 (in Chinese with English abstract).
18	吕林兰, 杜晓东, 王嫣 , 等, 2008. 马氏珠母贝3个野生种群及种群间杂交后代遗传多样性的ISSR分析[J]. 水生生物学报, 32(1):26-32.
	LÜ LINLAN, DU XIAODONG, WANG YAN , et al, 2008. Genetic diversity of three popultions and the first generations of hybridization between different populations of pearl oyster, Pinctada martensii (Dunker)[J]. Acta Hydrobiologica Sinica, 32(1):26-32 (in Chinese with English abstract).
19	曲艳波, 叶翚, 石耀华 , 等, 2006. 马氏珠母贝杂交选育群体遗传变异的RAPD分析[C]// 海南生物技术研究与发展研讨会论文集. 三亚: 中国生物工程学会: 27-34.
	QU YANBO, YE HUI, SHI YAOHUA , et al, 2006. RAPD analysis of genetic variation among cross breeding system of pearl oyster[C]// Proceedings of the Workshop on Research & Development of Biotechnology in Hainan. Sanya: Chinese Society of Biotechnology: 27-34 (in Chinese with English abstract).
20	沈恩健 , 2010. 利用AFLP和SSR对南海区域马氏珠母贝群体的遗传结构分析[D]. 湛江: 广东海洋大学.
	SHEN ENJIAN , 2010. Genetic diversrty analyses of Pinctada fucata (dunker) in south China sea using AFLP and SSR[D]. Zhanjiang: Guangdong Ocean University (in Chinese with English abstract).
21	苏天凤, 蔡云川, 张殿昌 , 等, 2002. 合浦珠母贝3个养殖群体的RAPD分析[J]. 中国水产科学, 9(2):106-109.
	SU TIANFENG, CAI YUNCHUAN, ZHANG DIANCHANG , et al, 2002. RAPD analysis of three cultured populations of Pinctada martensii[J]. Journal of Fishery Sciences of China, 9(2):106-109 (in Chinese with English abstract).
22	汤健, 管云雁, 刘文广 , 等, 2013. 马氏珠母贝家系遗传结构的微卫星分析[J]. 海洋科学, 37(8):35-41.
	TANG JIAN, GUAN YUNYAN, LIU WENGUANG , et al, 2013. Microsatellite DNA analysis of genetic structures about 9 families of Pinctada fucata[J]. Marine Sciences, 37(8):35-41 (in Chinese with English abstract).
23	童晓飞, 郭莹, 丛晓霏 , 2012. 高分辨溶解曲线及其在检测海洋经济贝类SNPs的应用前景[J]. 北京农业, ( 15):139.
	TONG XIAOFEI, GUO YING, CONG XIAOFEI , 2012. The high-resolution melting and the application foreground used in detecting SNPs of the biomonitor[J]. Beijing Agriculture, ( 15):139 (in Chinese with English abstract).
24	王爱民, 石耀华 , 2007. 马氏珠母贝的遗传改良技术[M] //王清印. 海水养殖生物的细胞工程育种. 北京: 海洋出版社: 133-164.
25	王爱民, 王嫣, 顾志峰 , 等, 2010. 马氏珠母贝(Pinctada martensii)2个地理群体杂交子代的杂种优势和遗传变异[J]. 海洋与湖沼, 41(1):140-147. doi: 10.11693/hyhz201001020020
	WANG AIMIN, WANG YAN, GU ZHIFENG , et al, 2010. Heterosis and genetic variation of hybrids from two geographical populations of pearl oyster, Pinctada martensii[J]. Oceanologia et Limnologia Sinica, 41(1):140-147 (in Chinese with English abstract). doi: 10.11693/hyhz201001020020
26	王家丰 , 2013. 长牡蛎基因区SNP标记规模开发及其在遗传育种研究中的应用[D]. 青岛: 中国科学院研究生院(海洋研究所).
	WANG JIAFENG , 2013. Large-scale development of SNP marker and its potential application in genetic breeding of Crassostrea gigas[D]. Qingdao: Institute of Oceanology, Chinese Academy of Sciences (in Chinese with English abstract).
27	王艳, 赵雪, 姜振峰 , 等, 2015. 大豆小片段法HRM基因分型体系优化[J]. 中国油料作物学报, 37(4):453-461. doi: 10.7505/j.issn.1007-9084.2015.04.004
	WANG YAN, ZHAO XUE, JIANG ZHENFENG , et al, 2015. Optimization of small amplicon of HRM genotyping system in soybean[J]. Chinese Journal of Oil Crop Sciences, 37(4):453-461 (in Chinese with English abstract). doi: 10.7505/j.issn.1007-9084.2015.04.004
28	杨润婷, 吴波, 李翀 , 等, 2013. 两种SNP分型方法的比较及其在柚品种鉴定中的应用[J]. 园艺学报, 40(6):1061-1070.
	ZHAO RUNTING, WU BO, LI CHONG , et al, 2013. Comparison of allele-specific PCR and high resolution melting analysis in SNP genotyping and their application in Pummelo cultivar identification[J]. Acta Horticulturae Sinica, 40(6):1061-1070 (in Chinese with English abstract).
29	喻达辉, 李有宁, 吴开畅 . 2005. 中国、日本和澳大利亚珍珠贝的ITS2序列特征分析[J]. 南方水产, ( 02):4-9.
	YU DAHUI, LI YOUNING, WU KAICHANG , 2005. Analysis on sequence variation of ITS 2 rDNA in Pinctada fucata from China, Japan and Australia[J]. South China Fisheries Science, 1(2):1-6 (in Chinese with English abstract).
30	张莉 , 2007. 中国珍珠产业的问题、困境与出路[J]. 农业现代化研究, 28(4):443-445.
	ZHANG LI , 2007. Economical analysis of dilemma of Chinese pearl industry and outlet[J]. Research of Agricultural Modernization, 28(4):443-445 (in Chinese with English abstract).
31	赵杰, 游新勇, 徐贞贞 , 等, 2018. SNP检测方法在动物研究中的应用[J]. 农业工程学报, 34(4):299-305.
	ZHAO JIE, YOU XINYONG, XU ZHENZHEN , et al, 2018. Review on application of SNP detection methods in animal research[J]. Transactions of the Chinese Society of Agricultural Engineering, 34(4):299-305 (in Chinese with English abstract).
32	赵琼一, 李信, 周德贵 , 等, 2010. 后基因组时代下作物的SNP分型方法[J]. 分子植物育种, 8(1):125-133.
	ZHAO QIONGYI, LIN XIN, ZHOU DEGUI , et al, 2010. SNP genotyping methods for crops in post-genomic era[J]. Molecular Plant Breeding, 8(1):125-133 (in Chinese with English abstract).
33	BOTSTEIN D, WHITE R L, SKOLNICK M , et al, 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 32(3):314-331.
34	GANAL M W, ALTMANN T, RÖDER M S , 2009. SNP identification in crop plants[J]. Current Opinion in Plant Biology, 12(2):211-217. doi: 10.1016/j.pbi.2008.12.009
35	HINE P M, THORNE T , 2000. A survey of some parasites and diseases of several species of bivalve mollusc in northern Western Australia[J]. Diseases of Aquatic Organisms, 40(1):67-78. doi: 10.3354/dao040067
36	HONG S P, SHIN S K, LEE E H , et al, 2008. High-resolution human papillomavirus genotyping by MALDI-TOF mass spectrometry[J]. Nature Protocols, 3(9):1476-1484. doi: 10.1038/nprot.2008.136
37	LIEW M, PRYOR R, PALAIS R , et al, 2004. Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons[J]. Clinical Chemistry, 50(7):1156-1164. doi: 10.1373/clinchem.2004.032136
38	LIU WENGUANG, HUANG XIANDE, LIN JIANSHI , et al, 2012. Seawater acidification and elevated temperature affect gene expression patterns of the pearl oyster Pinctada fucata[J]. PLoS One, 7(3):e33679. doi: 10.1371/journal.pone.0033679
39	MIYAKE T, ISOWA K, ISHIKAWA T , et al, 2016. Evaluation of genetic characteristics of wild and cultured populations of the Japanese pearl oyster Pinctada fucata martensii by using AFLP markers[J]. Aquaculture International, 24(2):537-548. doi: 10.1007/s10499-015-9943-2
40	MIYAZAKI T, GOTO K, KOBAYASHI T , et al, 1999. Mass mortalities associated with a virus disease in Japanese pearl oysters Pinctada fucata martensii[J]. Diseases of Aquatic Organisms, 37(1):1-12. doi: 10.3354/dao037001
41	MONTGOMERY J, WITTWER C T, PALAIS R , et al, 2007. Simultaneous mutation scanning and genotyping by high- resolution DNA melting analysis[J]. Nature Protocols, 2(1):59-66. doi: 10.1038/nprot.2007.10
42	NEFF M M, NEFF J D, CHORY J , et al, 1998. dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics[J]. The Plant Journal, 14(3):387-392. doi: 10.1046/j.1365-313X.1998.00124.x
43	NEWTON C R, GRAHAM A, HEPTINSTALL L E , et al, 1989. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)[J]. Nucleic Acids Research, 17(7):2503-2516. doi: 10.1093/nar/17.7.2503
44	NICKERSON M L, WEIRICH G, ZBAR B , et al, 2000. Signature-based analysis of MET proto-oncogene mutations using DHPLC[J]. Human Mutation, 16(1):68-76. doi: 10.1002/(ISSN)1098-1004
45	REED G H, WITTWER C T , 2004. Sensitivity and specificity of single-nucleotide polymorphism scanning by high- resolution melting analysis[J]. Clinical Chemistry, 50(10):1748-1754. doi: 10.1373/clinchem.2003.029751
46	SHAO KE, XIONG MEIHUA, XU NIAN , et al, 2013. Characterization of microsatellite loci in Sinilabeo rendahli and cross-amplification in four other Chinese cyprinid species[J]. Conservation Genetics Resource, 5(1):9-13. doi: 10.1007/s12686-012-9717-3
47	SOBRINO B, BRIÓN M, CARRACEDO A , 2005. SNPs in forensic genetics: a review on SNP typing methodologies[J]. Forensic Science International, 154(2-3):181-194. doi: 10.1016/j.forsciint.2004.10.020
48	TAMURA K, PETERSON D, PETERSON N , et al, 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 28(10):2731-2739. doi: 10.1093/molbev/msr121
49	WADA K T, KOMARU A , 1991. Estimation of genetic variation in shell traits of the Japanese pearl oyster[J]. Bulletin of National Research Institute of Aquaculture.( Japan)/Yoshokukenho, ( 20):19-24.
50	WADA K T, KOMARU A , 1994. Effect of selection for shell coloration on growth rate and mortality in the Japanese pearl oyster, Pinctada fucata martensii[J]. Aquaculture, 125(1-2):59-65. doi: 10.1016/0044-8486(94)90282-8
51	WEIR B S, COCKERHAM C C , 1984. Estimating F-statistics for the analysis of population structure[J]. Evolution, 38(6):1358-1370.
52	WITTWER C T , 2009. High-resolution DNA melting analysis: Advancements and limitations[J]. Human Mutation, 30(6):857-859. doi: 10.1002/humu.v30:6
53	WRIGHT S , 1931. Evolution in Mendelian populations[J]. Genetics, 16(2):97-159.
54	WRIGHT S , 1978. Evolution and the genetics of populations, vol. 4. variability within and among Populations[M]. Chicago: University of Chicago Press.
55	YEH F C, YANG R C, BOYLE T , et al, 1999. POPGENE, version 1.32: The user friendly software for population genetic analysis[CP]. Canada: University of Alberta.
56	YOKOGAWA K , 1998. Morphological variabilities and genetic features in Japanese common clam Ruditapes philippinarum[J]. Venus (Japanese Journal of Malacology), 57(2):121-132.
57	YU DAHUI, CHU KAHOU , 2006. Low genetic differentiation among widely separated populations of the pearl oyster Pinctada fucata as revealed by AFLP[J]. Journal of Experimental Marine Biology and Ecology, 333(1):140-146. doi: 10.1016/j.jembe.2005.12.046

位点名称	序列(5'-3')	SNP 类型	退火温度/℃	对应产物长度/bp
rs8	F: CCCAGCATACTTTATGGCAGAAT R: CCTAGCGTGGAGAAGAGATGTA	T/C	55	122
rs33	F: CAATACTCTCAGGGACATCTT R: CTCAACAGTGTCGCTTATATG	A/G	50	107
rs35	F: TTCCGCAGGATGAATTTAGGT R: TCTGAAGTCTGTTTGTCCAATG	C/A	53	103
rs44	F: CCAAAGCCAACTACACCCAAA R: GTCAAACGCCCACGATACAC	C/A	55	144

性状	父本	F1	母本
壳长	58.25±5.58^a	56.40±5.20^a	56.52±4.29^a
壳宽	23.44±2.23^a	20.23±2.84^b	21.72±2.43^ab
壳高	59.24±5.10^a	55.60±5.47^b	56.25±4.45^b
铰合线	51.16±3.79^a	50.17±4.97^a	50.84±4.28^a
总重	28.16±6.07^a	24.60±7.49^b	24.12±5.33^b
壳重	14.23±2.78^a	13.11±3.78^ab	11.95±2.62^b
软体部重	10.77±3.25^a	8.81±3.38^b	8.49±2.64^b
壳宽指数	0.17±0.01^a	0.15±0.01^b	0.16±0.01^ab
壳重指数	18.12±2.75^b	20.42±2.06^a	17.43±2.60^b
肥满度指数	13.36±2.27^a	13.28±2.53^a	13.02±2.25^a
壳高/壳长	1.02±0.05^a	0.99±0.05^b	1.00±0.06^ab
壳宽/壳长	0.41±0.03^a	0.36±0.03^b	0.38±0.03^ab
铰合线/壳长	0.88±0.05^b	0.89±0.05^ab	0.90±0.05^a

性状	负荷值
性状	主成分1	主成分2	主成分3
总重	0.969*	0.019	-0.040
软体部重	0.954*	0.021	0.107
壳高	0.937*	-0.052	-0.030
壳重	0.932*	-0.027	-0.103
铰合线长	0.901*	0.064	0.069
壳长	0.884*	0.067	-0.433
壳宽指数	0.113	0.952*	0.173
壳宽	0.301	0.938*	0.061
壳宽/壳长	0.102	0.936*	0.250
壳重指数	0.184	-0.758	0.088
肥满度	0.454	-0.531	0.393
铰合线/壳长	0.089	0.003	0.846*
壳高壳/长	0.250	-0.238	0.688*
贡献率/%	43.2	27.6	12.8

群体名称	位点	I	H_o	H_e	PIC	HWE P值
父本	rs8	0.3768	0.2500	0.2222	0.1948	0.4527
	rs33	0.6809	0.3438	0.4955	0.3685	0.0780
	rs35	0.3111	0.0625	0.1726	0.1555	0.0044*
	rs44	0.6211	0.1875	0.4365	0.3384	0.0010*
平均值		0.4975	0.2110	0.3317	0.2643
F1	rs8	0.6002	0.1515	0.4163	0.3260	0.0003*
	rs33	0.6816	0.3030	0.4960	0.3692	0.0230
	rs35	0.5860	0.4242	0.4028	0.3182	0.7500
	rs44	0.6635	0.2727	0.4779	0.3598	0.0120
平均值		0.6328	0.2879	0.4483	0.3433
母本	rs8	0.6881	0.4333	0.5034	0.3724	0.4380
	rs33	0.5799	0.3333	0.3977	0.3137	0.3622
	rs35	0.6657	0.0333	0.4808	0.3614	0.0000*
	rs44	0.6926	0.2333	0.4921	0.3747	0.0025*
平均值		0.6566	0.2583	0.4685	0.3556

群体名称	基因型(频率)
群体名称	rs8	rs33	rs35	rs44
父本	TT(0.75)	GG(0.41)	CC(0.88)	CC(0.59)
	TC(0.25)	GA(0.34)	CA(0.06)	CA(0.19)
	CC(0)	AA(0.25)	AA(0.06)	AA(0.22)
F1	TT(0.64)	GG(0.27)	CC(0.52)	CC(0.24)
	TC(0.15)	GA(0.30)	CA(0.42)	CA(0.27)
	CC(0.21)	AA(0.42)	AA(0.06)	AA(0.49)
母本	TT(0.23)	GG(0.10)	CC(0.60)	CC(0.37)
	TC(0.43)	GA(0.33)	CA(0.03)	CA(0.23)
	CC(0.33)	AA(0.57)	AA(0.37)	AA(0.40)

形态学和SNP标记分析马氏珠母贝杂交子代及其亲本群体的遗传结构

Morphological and SNP markers for analysis of genetic structure of hybrid progeny and their parental populations of Pinctada fucata martensii

RichHTML

PDF (PC)

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 57

相关文章 15

Metrics

本文评价

推荐阅读 0

群体	数量	预测分类			判别准确率		综合判别率
群体	数量	父本	F1	母本	P1	P2	综合判别率
父本	50	36	9	5	72%	75%	72%
F1	50	8	32	10	64%	68.1%
母本	50	4	6	40	80%	72.7%

[1]	李能辉, 黄庆, 李航, 曾俊, 吴科锋, 谭华强. 基于形态和分子数据对湛江海域4个江蓠物种(红藻门)的分类学研究[J]. 热带海洋学报, 2024, 43(2): 34-47.
[2]	韩通, 李晶晶, 刘正一, 刘凯, 张金浩, 秦松, 钟志海. 红纤维虾形草(Phyllospadix iwatensis)种子形态及内部特征[J]. 热带海洋学报, 2022, 41(6): 105-113.
[3]	许瀚之, 张华, 熊盼盼, 何毛贤. 马氏珠母贝异速生长个体对免疫刺激的差异响应[J]. 热带海洋学报, 2022, 41(5): 180-188.
[4]	刘金梅, 黄冰心, 丁兰平, 王雪聪, 闫璟, 闫盼竹, 张瑶. 我国海南省东南部台湾那屿藻的形态观察及系统发育分析[J]. 热带海洋学报, 2021, 40(6): 76-82.
[5]	邹聪聪, 王丽娟, 吴志昊, 尤锋. 基于线粒体控制区序列的黄海日本鳀(Engraulis japonicus)的群体遗传结构^*[J]. 热带海洋学报, 2021, 40(5): 25-35.
[6]	任慧敏, 张衡, 许莎莎, 李圣法, 李建生, 李治洪, 何利军. 基于线粒体控制区的中国近海竹荚鱼种群遗传结构和种群历史动态分析[J]. 热带海洋学报, 2021, 40(5): 36-44.
[7]	牛彪彪, 翟梦怡, 李扬. 塞舌尔角毛藻的形态学再描述和分子系统学分析[J]. 热带海洋学报, 2021, 40(4): 44-49.
[8]	龙超, 罗肇河, 韦章良, 杨芳芳, 李茹, 龙丽娟. 海南三亚鹿回头虫黄藻(Effrenium voratum)的形态学和系统发育学研究^*[J]. 热带海洋学报, 2021, 40(4): 35-43.
[9]	潘肖兰, 刘惠茹, 许濛, 许瀚之, 张华, 何毛贤. 马氏珠母贝PfAQP4免疫功能初探[J]. 热带海洋学报, 2021, 40(2): 83-89.
[10]	刘金梅, 姜晶晶, 马鑫, 黄冰心, 杨楠, 刘美媛, 丁兰平. 我国粤东沙菜属(红藻门杉藻目)的分类鉴定 ^*[J]. 热带海洋学报, 2021, 40(1): 99-110.
[11]	黄景, 欧泽奎, 刘文广, 何毛贤. 基于SLAF测序分析3个塔形马蹄螺群体的遗传结构和多样性*[J]. 热带海洋学报, 2020, 39(5): 1-18.
[12]	李敏, 孔啸兰, 许友伟, 陈作志. 基于线粒体控制区序列的花斑蛇鲻遗传多态性分析[J]. 热带海洋学报, 2020, 39(4): 42-49.
[13]	黄丽芬, 李群, 吕颂辉, 张亮, 谢学东. 中国西沙群岛底栖甲藻热带库里亚藻(Coolia tropicalis)的形态学、系统发育及毒性研究[J]. 热带海洋学报, 2020, 39(3): 86-97.
[14]	潘肖兰, 刘惠茹, 许濛, 许瀚之, 张华, 何毛贤. 马氏珠母贝水通道蛋白基因AQP4 cDNA克隆和表达分析[J]. 热带海洋学报, 2020, 39(3): 66-75.
[15]	江丽春, 李群, 吕颂辉. 广西涠洲岛底栖甲藻前沟藻属(Amphidinium)种类的形态学和系统发育学研究[J]. 热带海洋学报, 2020, 39(3): 106-115.

	遗传距离和遗传相似系数			基因流和遗传分化系数
	父本	F1	母本	父本	F1	母本
父本	****	0.9320	0.8387	****	0.0546	0.1128
F1	0.0704	****	0.9947	4.3262	****	0.0166
母本	0.1759	0.0546	****	1.9662	7.7701	****