基于线粒体控制区序列的黄海日本鳀(Engraulis japonicus)的群体遗传结构*

doi:10.11978/2020119

热带海洋学报 ›› 2021, Vol. 40 ›› Issue (5): 25-35.doi: 10.11978/2020119CSTR: 32234.14.2020119

基于线粒体控制区序列的黄海日本鳀(Engraulis japonicus)的群体遗传结构^*

邹聪聪^1,^2,³(), 王丽娟^1,², 吴志昊^1,², 尤锋^1,²()

1.中国科学院实验海洋生物学重点实验室, 海洋大科学中心(中国科学院海洋研究所), 山东青岛 266071
2.青岛海洋科学与技术试点国家实验室实验海洋生物学与生物技术实验室, 山东青岛 266237
3.中国科学院大学, 北京 100049

收稿日期:2020-10-15 修回日期:2021-01-29 出版日期:2021-09-10 发布日期:2021-02-09
通讯作者: 尤锋
作者简介:邹聪聪(1994—), 女, 浙江省丽水市人, 博士研究生, 主要从事海水鱼类遗传学研究。email: zoucongcong17@mails.ucas.ac.cn
基金资助:
科技基础性工作专项(2014FY110500);国家海洋水产种质资源库项目(2017DKA30470)

Population genetic structure of Japanese anchovy (Engraulis japonicus) in the Yellow Sea based on mitochondrial control region sequences^*

ZOU Congcong^1,^2,³(), WANG Lijuan^1,², WU Zhihao^1,², YOU Feng^1,²()

1. Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2. Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
3. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2020-10-15 Revised:2021-01-29 Online:2021-09-10 Published:2021-02-09
Contact: YOU Feng
Supported by:
Special Program for Basic Research of the Ministry of Science and Technology of China(2014FY110500);National Marine Genetic Resource Center(2017DKA30470)

摘要/Abstract

摘要：

遗传多样性和群体遗传结构是研究群体动态变化的重要内容, 也是种质资源评估与保护的基础。日本鳀(Engraulis japonicus)是我国东部近海的重要鱼种, 具有重要的生态价值和经济价值。文章利用线粒体控制区全序列分析了黄海海域4个地理群体(北黄海北部、北黄海南部、南黄海北部和南黄海中部)日本鳀的遗传多样性、种群遗传结构和历史动态变化。结果显示, 131个样品检测到了126个单倍型, 且4个群体的单倍型多样性均很高, 其中北黄海南部群体的最低(0.995±0.009), 南黄海中部群体的最高(1.000±0.014)。而核苷酸多样性均较低, 为0.010±0.005 ~ 0.011±0.006。主成分判别分析(DAPC)和遗传分化系数F_st表明4个群体无明显的群体分化现象, 群体间的遗传同质性水平高, 分子方差分析(AMOVA)也显示分子变异基本来自于群体内, 并且没有明显的群体遗传结构。贝叶斯系统发育树分析发现, 黄海日本鳀有2个谱系, 谱系1和谱系2的分化时间为0.701Ma前, 可以追溯到更新世期间; 进一步中性检验和核苷酸错配分布分析表明这2个谱系可能发生过群体扩张。贝叶斯天际线图则显示黄海鳀鱼的有效群体数量在0.150Ma前发生了明显的下降。

关键词: 黄海, 日本鳀, 遗传多样性, 群体遗传结构, 更新世

Abstract:

Genetic diversity and population structure are important aspects in population dynamics study, and are the basis of fish resources evaluation and protection. Japanese anchovy (Engraulis japonicus) is an important fish species in the China East Coast, which has both ecological and economic values. In this study, the whole sequence of mitochondrial control region was used to analyze the genetic diversity, population genetic structure, and demographic history of its four geographical populations in the Yellow Sea, namely, north of the northern Yellow Sea (NYN), south of the northern Yellow Sea (NYS), north of the southern Yellow Sea (SYN), and central part of the southern Yellow Sea (SYC). A total of 126 haplotypes were detected in 131 samples of the four populations, and their haplotype diversity levels were high, among which the lowest was in the NYS (0.995±0.009) and the highest was in the SYC (1.000±0.014). In all populations, the nucleotide diversity was low (0.010±0.005 to 0.011±0.006). The results of Discriminant Analysis of Principal Components (DAPC) and F_st values indicated that there was no significant segregation among the four populations, and the level of genetic homogeneity among populations was high. There was no obvious population genetic structure, with the large molecular variation within the populations based on the Analysis of Molecular Variance (AMOVA). The Bayesian phylogenetic tree revealed two distinct lineages, which diverged at 0.701 million years ago, dating back to the Pleistocene period. Neutrality test and nucleotide mismatch distribution analyses demonstrated that these two lineages experienced demographic expansions. The effective population of Japanese anchovy in the Yellow Sea decreased significantly at 0.150 million years ago by analyzing the Bayesian skyline.

Key words: Yellow Sea, Engraulis japonicus, genetic diversity, population genetic structure, Pleistocene

中图分类号:

P735.54

邹聪聪, 王丽娟, 吴志昊, 尤锋. 基于线粒体控制区序列的黄海日本鳀(Engraulis japonicus)的群体遗传结构^*[J]. 热带海洋学报, 2021, 40(5): 25-35.

ZOU Congcong, WANG Lijuan, WU Zhihao, YOU Feng. Population genetic structure of Japanese anchovy (Engraulis japonicus) in the Yellow Sea based on mitochondrial control region sequences^*[J]. Journal of Tropical Oceanography, 2021, 40(5): 25-35.

图/表 11

图1

表1

图2

图3

表2

表3

图4

表4

表5

图5

图6

参考文献 60

[1]	成庆泰, 郑葆珊, 1987. 中国鱼类系统检索[M]. 北京: 科学出版社: 59-61.
	CHENG QINGTAI, ZHENG BAOSHAN, 1987. Systematic synopsis of Chinese fishes[M]. Beijing: Science Press: 59-61(in Chinese).
[2]	高天翔, 高兵兵, 李忠炉, 等, 2020. 基于线粒体DNA控制区序列的短棘鲾群体遗传学[J]. 水产学报, 44(5):715-722.
	GAO TIANXIANG, GAO BINGBING, LI ZHONGLU, et al, 2020. Population genetics study of Leiognathus equulus based on the control region fragment of mitochondrial DNA[J]. Journal of Fisheries of China, 44(5):715-722 (in Chinese with English abstract).
[3]	胡东方, 2009. 黄海鳀鱼的摄食生态学研究[D]. 青岛: 中国海洋大学: 1-2.
	HU DONGFANG, 2009. Studies on feeding ecology of anchovy in Yellow Sea[D]. Qingdao: Ocean University of China: 1-2 (in Chinese with English abstract).
[4]	李秀梅, 叶振江, 李增光, 等, 2016. 黄海中部近岸产卵场日本鳀卵子大小的时空变化[J]. 中国海洋大学学报, 46(2):54-60.
	LI XIUMEI, YE ZHENJIANG, LI ZENGGUANG, et al, 2016. Spatial and temporal changes of the egg Size of Japanese Anchovy (Engraulis japonicus) inhabiting the near shore spawning ground of central Yellow Sea[J]. Periodical of Ocean University of China, 46(2):54-60 (in Chinese with English abstract).
[5]	梁述章, 宋炜, 马春艳, 等, 2019. 基于线粒体控制区的中国近海棘头梅童鱼群体遗传结构研究[J]. 海洋渔业, 41(22):138-148.
	LIANG SHUZHANG, SONG WEI, MA CHUNYAN, et al, 2019. Genetic structure of Collichthys lucidus populations from China coastal waters based on mt DNA control region[J]. Marine Fisheries, 41(2):138-148 (in Chinese with English abstract).
[6]	唐启升, 苏纪兰, 2000. 中国海洋生态系统动力学研究. Ⅰ. 关键科学问题与研究发展战略[M]. 北京: 科学出版社: 45-72(in Chinese).
[7]	吴仁协, 张浩冉, 牛素芳, 等, 2019. 东海近岸带鱼(Trichiurus japonicus)线粒体控制区序列的群体遗传变异研究[J]. 海洋与湖沼, 50(6):1318-1327.
	WU RENXIE, ZHANG HAORAN, NIU SUFANG, et al, 2019. Study on population genetic variation of Trichiurus japonicus in nearshore of the East China Sea in mitochondrial control region sequences[J]. Oceanologia et Limnologia Sinica, 50(6):1318-1327 (in Chinese with English abstract).
[8]	薛利建, 刘子藩, 2005. 东海日本鳀数量分布和生物学特性的研究[J]. 浙江海洋学院学报(自然科学版), 24(4):312-317.
	XUE LIJIAN, LIU ZIFAN, 2005. Study on the biomass distribution & biological characteristics of Engraulis japonicus in the East China Sea[J]. Journal of Zhejiang Ocean University (Natural Science), 24(4):312-317 (in Chinese with English abstract).
[9]	赵爽, 章群, 乐小亮, 等, 2010. 中国近海5个黑鲷地理群体的遗传变异[J]. 海洋科学, 34(2):75-79.
	ZHAO SHUANG, ZHANG QUN, YUE XIAOLIANG, et al, 2010. Genetic variation among 5 stocks of Acanthopagrus schlegeli in China’s coastal waters[J]. Marine Sciences, 34(2):75-79 (in Chinese with English abstract).
[10]	赵宪勇, 2006. 黄海鳀鱼种群动力学特征及其资源可持续利用[D]. 青岛: 中国海洋大学: 18-43.
	ZHAO XIANYONG, 2006. Population dynamic characteristics and sustainable utilization of the anchovy in the Yellow Sea[D]. Qingdao: Ocean University of China: 18-43 (in Chinese with English abstract).
[11]	朱琨, 刘微, 2020. 一种鳀鱼提取物的开发和生产工艺研究[J]. 中国食品添加剂, 31(5):69-74.
	ZHU KUN, LIU WEI, 2020. The development and technology of anchovy extract production[J]. China Food Additives, 31(5):69-74 (in Chinese with English abstract).
[12]	BOUCKAERT R, HELED J, KÜHNERT D, et al, 2014. Beast 2: a software platform for bayesian evolutionary analysis[J]. PLoS Computational Biology, 10(4):e1003537. doi: 10.1371/journal.pcbi.1003537
[13]	CANALES-AGUIRRE C B, FERRADA-FUENTES S, GALLEGUILLOS R, et al, 2018. High genetic diversity and low-population differentiation in the patagonian sprat (Sprattus fuegensis) based on mitochondrial DNA[J]. Mitochondrial DNA Part A, 29(8):1148-1155. doi: 10.1080/24701394.2018.1424841
[14]	CHECKLEY JR D M, ASCH R G, RYKACZEWSKI R R, 2017. Climate, anchovy, and sardine[J]. Annual Review of Marine Science, 9:469-493. doi: 10.1146/annurev-marine-122414-033819
[15]	CHENG JIAO, HAN ZHIQIANG, SONG NA, et al, 2018. Effects of Pleistocene glaciation on the phylogeographic and demographic histories of chub mackerel Scomber japonicus in the north-western Pacific[J]. Marine and Freshwater Research, 69(4):514-524. doi: 10.1071/MF17099
[16]	CLARKE C R, KARL S A, HORN R L, et al, 2015. Global mitochondrial DNA phylogeography and population structure of the silky shark, Carcharhinus falciformis[J]. Marine Biology, 162(5):945-955. doi: 10.1007/s00227-015-2636-6
[17]	DARRIBA D, TABOADA G L, DOALLO R, et al, 2012. jModelTest 2: more models, new heuristics and parallel computing[J]. Nature Methods, 9(8):772.
[18]	DOMINGUES R R, HILSDORF A W S, SHIVJI M M, et al, 2018. Effects of the Pleistocene on the mitochondrial population genetic structure and demographic history of the silky shark (Carcharhinus falciformis) in the western Atlantic Ocean[J]. Reviews in Fish Biology and Fisheries, 28(1):213-227. doi: 10.1007/s11160-017-9504-z
[19]	DONALDSON K A, WILSON JR R R, 1999. Amphi-panamic geminates of snook (Percoidei: Centropomidae) provide a calibration of the divergence rate in the mitochondrial DNA control region of fishes[J]. Molecular Phylogenetics and Evolution, 13(1):208-213. doi: 10.1006/mpev.1999.0625
[20]	EXCOFFIER L, LISCHER H E L, 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources, 10(3):564-567. doi: 10.1111/men.2010.10.issue-3
[21]	GRANT W A S, BOWEN B W, 1998. Shallow population histories in deep evolutionary lineages of marine fishes: Insights from sardines and anchovies and lessons for conservation[J]. Journal of Heredity, 89(5):415-426. doi: 10.1093/jhered/89.5.415
[22]	GUO SHANSHAN, ZHANG GUIRONG, GUO XIANGZHAO, et al, 2014. Genetic diversity and population structure of Schizopygopsis younghusbandi Regan in the Yarlung Tsangpo River inferred from mitochondrial DNA sequence analysis[J]. Biochemical Systematics and Ecology, 57:141-151. doi: 10.1016/j.bse.2014.07.026
[23]	HAN ZHIQIANG, GAO TIANXIANG, YANAGIMOTO T, et al, 2008. Genetic population structure of Nibea albiflora in Yellow Sea and East China Sea[J]. Fisheries Science, 74(3):544-552. doi: 10.1111/j.1444-2906.2008.01557.x
[24]	HAYASHI A, ZHANG K, SARUWATARI T, et al, 2016. Distribution of eggs and larvae of Japanese anchovy Engraulis japonicus in the Pacific waters off northern Japan in summer[J]. Fisheries Science, 82(2):311-319. doi: 10.1007/s12562-015-0966-4
[25]	INOUE K, MONROE E M, ELDERKIN C L, et al, 2014. Phylogeographic and population genetic analyses reveal Pleistocene isolation followed by high gene flow in a wide ranging, but endangered, freshwater mussel[J]. Heredity, 112(3):282-290. doi: 10.1038/hdy.2013.104
[26]	JAMALUDIN N A, MOHD-ARSHAAD W, AKIB N A M, et al, 2020. Phylogeography of the Japanese scad, Decapterus maruadsi (Teleostei; Carangidae) across the Central Indo-West Pacific: evidence of strong regional structure and cryptic diversity[J]. Mitochondrial DNA Part A, 31(7):298-310. doi: 10.1080/24701394.2020.1799996
[27]	JUNG S, PANG I-C, LEE J-H, et al, 2016. Climate-change driven range shifts of anchovy biomass projected by bio-physical coupling individual based model in the marginal seas of East Asia[J]. Ocean Science Journal, 51(4):563-580. doi: 10.1007/s12601-016-0055-3
[28]	KASIM N S, JAAFAR T N A M, PIAH R M, et al, 2020. Recent population expansion of longtail tuna Thunnus tonggol (Bleeker, 1851) inferred from the mitochondrial DNA markers[J]. PeerJ, 8:e9679. doi: 10.7717/peerj.9679
[29]	KETCHUM R N, DIENG M M, VAUGHAN G O, et al, 2016. Levels of genetic diversity and taxonomic status of Epinephelus species in United Arab Emirates fish markets[J]. Marine Pollution Bulletin, 105(2):540-545. doi: 10.1016/j.marpolbul.2015.11.042
[30]	KIM J Y, CHO E S, KIM W J, 2004. Population genetic structure of Japanese Anchovy (Engraulis japonicus) in Korean waters based on mitochondrial 12S ribosomal RNA gene sequences[J]. Journal of Life Science, 14(6):938-950. doi: 10.5352/JLS.2004.14.6.938
[31]	KWON D-H, HWANG S-D, LIM D, 2012. Catch predictions for Pacific anchovy Engraulis japonicus larvae in the Yellow Sea[J]. Fisheries and Aquatic Sciences, 15(4):345-352. doi: 10.5657/FAS.2012.0345
[32]	LARKIN M A, BLACKSHIELDS G, BROWN N P, et al, 2007. Clustal W and Clustal X version 2.0[J]. Bioinformatics, 23(21):2947-2948. doi: 10.1093/bioinformatics/btm404
[33]	LEIGH J W, BRYANT D, 2015. POPART: full-feature software for haplotype network construction[J]. Methods in Ecology and Evolution, 6(9):1110-1116. doi: 10.1111/mee3.2015.6.issue-9
[34]	LIU JINXIAN, GAO TIANXIANG, ZHUANG ZHIMENG, et al, 2006. Late Pleistocene divergence and subsequent population expansion of two closely related fish species, Japanese anchovy (Engraulis japonicus) and Australian anchovy (Engraulis australis)[J]. Molecular Phylogenetics and Evolution, 40(3):712-723. doi: 10.1016/j.ympev.2006.04.019
[35]	LIU JINXIAN, GAO TIANXIANG, WU SHIFANG, et al, 2007. Pleistocene isolation in the Northwestern Pacific marginal seas and limited dispersal in a marine fish, Chelon haematocheilus (Temminck & Schlegel, 1845)[J]. Molecular Ecology, 16(2):275-288. doi: 10.1111/j.1365-294X.2006.03140.x
[36]	MA CHUNYAN, CHENG QIQUN, ZHANG QINGYI, et al, 2010. Genetic variation of Coilia ectenes (Clupeiformes: Engraulidae) revealed by the complete cytochrome b sequences of mitochondrial DNA[J]. Journal of Experimental Marine Biology and Ecology, 385(1-2):14-19. doi: 10.1016/j.jembe.2010.01.015
[37]	MENEZES M R, KUMAR G, KUNAL S P, 2012. Population genetic structure of skipjack tuna Katsuwonus pelamis from the Indian coast using sequence analysis of the mitochondrial DNA D-loop region[J]. Journal of Fish Biology, 80(6):2198-2212. doi: 10.1111/j.1095-8649.2012.03270.x
[38]	MONTES I, IRIONDO M, MANZANO C, et al, 2017. Development of gene-associated single nucleotide polymorphisms for Japanese anchovy Engraulis japonicus through cross-species amplification[J]. Fisheries Science, 84(1):1-7. doi: 10.1007/s12562-017-1134-9
[39]	MUNIAN K, BHASSU S, 2015. Genetic structure of locally threatened cyprinid, Osteochilus melanopleurus, in Peninsular Malaysia River systems inferred from mitochondrial DNA control region[J]. Biochemical Systematics and Ecology, 61:336-343. doi: 10.1016/j.bse.2015.06.034
[40]	MYOUNG S H, KIM J-K, 2014. Genetic diversity and population structure of the gizzard shad, Konosirus punctatus (Clupeidae, Pisces), in Korean waters based on mitochondrial DNA control region sequences[J]. Genes & Genomics, 36(5):591-598.
[41]	NEI M, 1987. Molecular evolutionary genetics[M]. New York: Columbia University Press.
[42]	NYKÄNEN M, DILLANE E, REID D, et al, 2020. Genetic methods reveal high diversity and no evidence of stock structure among cuckoo rays (Leucoraja naevus) in the northern part of Northeast Atlantic[J]. Fisheries Research, 232:105715. doi: 10.1016/j.fishres.2020.105715
[43]	OHATA R, MASUDA R, YAMASHITA Y, 2018. Ontogeny of turbiditaxis in hatchery-reared Japanese anchovy Engraulis japonicus[J]. Journal of Fish Biology, 92(4):1235-1240. doi: 10.1111/jfb.2018.92.issue-4
[44]	OTWOMA L M, REUTER H, TIMM J, et al, 2018. Genetic connectivity in a herbivorous coral reef fish (Acanthurus leucosternon Bennet, 1833) in the eastern African region[J]. Hydrobiologia, 806(1):237-250. doi: 10.1007/s10750-017-3363-4
[45]	PANDEY D, RYU Y-W, MATSUBARA T, 2017. Features of sperm motility and circadian rhythm in Japanese anchovy (Engraulis japonicus)[J]. Fisheries and Aquaculture Journal, 8(2):203.
[46]	PEDROSA-GERASMIO I R, AGMATA A B, SANTOS M D, 2015. Genetic diversity, population genetic structure, and demographic history of Auxis thazard (Perciformes), Selar crumenophthalmus (Perciformes), Rastrelliger kanagurta (Perciformes) and Sardinella lemuru (Clupeiformes) in Sulu-Celebes Sea inferred by mitochondrial DNA sequences[J]. Fisheries Research, 162:64-74. doi: 10.1016/j.fishres.2014.10.006
[47]	PILLANS B, CHAPPELL J, NAISH T R, 1998. A review of the Milankovitch climatic beat: template for Plio-Pleistocene sea-level changes and sequence stratigraphy[J]. Sedimentary Geology, 122(1-4):5-21. doi: 10.1016/S0037-0738(98)00095-5
[48]	RONQUIST F, TESLENKO M, VAN DER MARK P, et al, 2012. MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space[J]. Systematic Biology, 61(3):539-542. doi: 10.1093/sysbio/sys029
[49]	SEFC K M, WAGNER M, ZANGL L, et al, 2020. Phylogeographic structure and population connectivity of a small benthic fish (Tripterygion tripteronotum) in the Adriatic Sea[J]. Journal of Biogeography, 47(11):2502-2517. doi: 10.1111/jbi.v47.11
[50]	SHEN K-N, JAMANDRE B W, HSU C-C, et al, 2011. Plio-Pleistocene sea level and temperature fluctuations in the Northwestern Pacific promoted speciation in the globally-distributed flathead mullet Mugil cephalus[J]. BMC Evolutionary Biology, 11(1):83. doi: 10.1186/1471-2148-11-83
[51]	SUN CHAO, XUAN ZHONGYA, LIU HONGBO, et al, 2019. Cyt b gene and D-loop sequence analyses of Coilia nasus from the Rokkaku River of Japan[J]. Regional Studies in Marine Science, 32:100840. doi: 10.1016/j.rsma.2019.100840
[52]	TAKAHASHI M, WATANABE Y, 2005. Effects of temperature and food availability on growth rate during late larval stage of Japanese anchovy (Engraulis japonicus) in the Kuroshio-Oyashio transition region[J]. Fisheries Oceanography, 14(3):223-235.
[53]	WANG QIAN, ZHANG JIE, MATSUMOTO H, et al, 2016. Population structure of elongate ilisha Ilisha elongata along the Northwestern Pacific Coast revealed by mitochondrial control region sequences[J]. Fisheries Science, 82(5):771-785. doi: 10.1007/s12562-016-1018-4
[54]	XING QINWANG, YU HUAMING, YU HAIQING, et al, 2020. A comprehensive model-based index for identification of larval retention areas: a case study for Japanese anchovy Engraulis japonicus in the Yellow Sea[J]. Ecological Indicators, 116:106479. doi: 10.1016/j.ecolind.2020.106479
[55]	YU HAIQING, YU HUAMING, ITO S-I, et al, 2020. Potential environmental drivers of Japanese anchovy (Engraulis japonicus) recruitment in the Yellow Sea[J]. Journal of Marine Systems, 212:103431. doi: 10.1016/j.jmarsys.2020.103431
[56]	YU ZINIU, KONG XIAOYU, GUO TIANHUI, et al, 2005. Mitochondrial DNA sequence variation of Japanese anchovy Engraulis japonicus from the Yellow Sea and East China Sea[J]. Fisheries Science, 71(2):299-307. doi: 10.1111/fis.2005.71.issue-2
[57]	ZHANG YANHONG, PHAM N K, ZHANG HUIXIAN, et al, 2014. Genetic variations in two seahorse species (Hippocampus mohnikei and Hippocampus trimaculatus): Evidence for middle pleistocene population expansion[J]. PLoS One, 9(8):e105494. doi: 10.1371/journal.pone.0105494
[58]	ZHANG YURONG, YANG FANG, WANG ZHAOLIN, et al, 2017. Mitochondrial DNA variation and population genetic structure in the small yellow croaker at the coast of Yellow Sea and East China Sea[J]. Biochemical Systematics and Ecology, 71:236-243. doi: 10.1016/j.bse.2017.03.003
[59]	ZHENG WEI, ZOU LI, HAN ZHIQIANG, 2015. Genetic analysis of the populations of Japanese anchovy Engraulis japonicus from the Yellow Sea and East China Sea based on mitochondrial cytochrome b sequence[J]. Biochemical Systematics and Ecology, 58:169-177. doi: 10.1016/j.bse.2014.12.007
[60]	ZHOU XIN, SUN YAO, HUANG WEN, et al, 2015. The Pacific decadal oscillation and changes in anchovy populations in the Northwest Pacific[J]. Journal of Asian Earth Sciences, 114:504-511. doi: 10.1016/j.jseaes.2015.06.027

变异来源	平方和	变异百分比	F 统计	p
一个基因池
群体间	16.901	0.190
群体内	673.710	99.810	F_st = 0.00192	0.341
两个基因池(NYN、NYS)(SYN、SYC)
组群间	3.759	-0.84	F_ct = -0.00841	1.000
组群内群体间	13.141	0.74	F_sc = 0.00734	0.131
群体内	673.710	100.10	F_st = -0.00101	0.286
两个基因池(NYN、NYS、SYN)(SYC)
组群间	6.157	0.41	F_ct= 0.00414	0.483
组群内群体间	10.743	0.03	F_sc= 0.00035	0.392
群体内	673.710	99.55	F_st = 0.00448	0.329

群体	Tajima’s D		Fu’s F_s		Ramos-Onnsis and Roza’s R₂
群体	D	p	F_s	p	R₂	p
NYN	-1.307	0.073	-24.603	0.000	0.107	0.000
NYS	-1.013	0.162	-24.077	0.000	0.113	0.000
SYN	-1.348	0.070	-24.249	0.000	0.118	0.000
SYC	-0.702	0.284	-13.580	0.000	0.127	0.000
谱系 1	-1.532	0.034	-24.790	0.000	0.103	0.000
谱系 2	-1.435	0.041	-24.619	0.000	0.099	0.000

群体		群体扩张模型					空间扩张模型
群体	τ	t	θ₀	θ₁	SSD	HRI	τ	θ	M	SSD	HRI
NYN	10.578	0.172	0.000	1527.500	0.001	0.007	10.585	0.010	1574.756	0.001	0.007
NYS	9.396	0.153	1.331	386.250	0.002	0.009	9.232	1.480	206.480	0.002	0.009
SYN	9.855	0.160	0.000	280.625	0.003	0.011	8.745	1.201	99999.000	0.003	0.011
SYC	11.881	0.193	0.002	279.688	0.004	0.010	11.802	0.026	99999.000	0.004	0.010
谱系1	8.326	0.135	0.228	99999.000	0.001	0.010	8.543	0.103	99999.000	0.001	0.010
谱系2	10.109	0.164	0.025	118.896	0.001	0.005	9.189	0.860	287.179	0.001	0.005

基于线粒体控制区序列的黄海日本鳀(Engraulis japonicus)的群体遗传结构^*

Population genetic structure of Japanese anchovy (Engraulis japonicus) in the Yellow Sea based on mitochondrial control region sequences^*

RichHTML

PDF (PC)

赞