菱形藻株系MD1的脂肪酸组成及其在NaHCO3、NaCl和pH值影响下的生长特征

doi:10.11978/j.issn.1009-5470.2010.06.079

热带海洋学报 ›› 2010, Vol. 29 ›› Issue (6): 79-85.doi: 10.11978/j.issn.1009-5470.2010.06.079cstr: 32234.14.j.issn.1009-5470.2010.06.079

菱形藻株系MD1的脂肪酸组成及其在NaHCO3、NaCl和pH值影响下的生长特征

苏娇娇1, 2, 向文洲1, 萧邶1, 徐少琨1, 何慧1

1. 中国科学院南海海洋研究所, 广东广州 510301; 2. 中国科学院研究生院, 北京 100049

收稿日期:2010-03-25 修回日期:2010-04-29 出版日期:2010-12-15 发布日期:2010-12-15
通讯作者: 向文洲。
作者简介:苏娇娇(1982—), 女, 河北省晋州市人, 硕士研究生, 主要从事微藻生物技术研究。E-mail: sjjsusan@gmail.com
基金资助:
广东省海洋与渔业科技项目(A200899I01); 广东省科技计划项目(2009B080701093)

Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain

SU Jiao-jiao1,2, XIANG Wen-zhou1, XIAO Bei1, XU Shao-kun1, HE Hui1

1. South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate School of the CAS, Beijing 100049, China

Received:2010-03-25 Revised:2010-04-29 Online:2010-12-15 Published:2010-12-15
Contact: 向文洲。
About author:苏娇娇(1982—), 女, 河北省晋州市人, 硕士研究生, 主要从事微藻生物技术研究。E-mail: sjjsusan@gmail.com
Supported by:
广东省海洋与渔业科技项目(A200899I01); 广东省科技计划项目(2009B080701093)

摘要/Abstract

摘要：

研究了具有嗜碱特性的菱形藻Nitzschia sp. (MD1)株系的脂肪酸组分、含量以及不同NaHCO3、NaCl和pH值水平下对其生长的影响。GC-MS测定结果显示, 在NaHCO3浓度为0.2mol•L?1、NaCl浓度为30g•L?1、初始pH值为9.5时, MD1的EPA含量占干重的3.02%, 占总脂肪酸的比例为17.93%。生长测定结果表明, MD1具有极强的耐碱能力, 其生长适宜的NaHCO3浓度为0.1—0.2mol•L?1, 在0.3mol•L?1的NaHCO3浓度下仍能良好生长; MD1最适的起始pH值范围为7.5—9.5, 其生长速率在pH7.5与pH9.5之间没有显著性差异(P>0.05), 可以耐受pH值为11.5的高碱性环境并保持较好的生长; MD1显示广盐适应性, NaCl浓度在0g•L?1时获得最大比生长速率, 在30—40g•L?1时对其生长没有显著影响(P>0.05), 在NaCl浓度30g•L?1、pH 8.5—9.5及NaHCO3 0.2mol•L?1时, 获得了最大比生长速率0.56d?1。由此可见, MD1可望具有进行大规模培养开发EPA的前景。

关键词: 菱形藻Nitzschia sp., 嗜碱特性, pH, NaHCO3, EPA

Abstract:

In this study, we investigated the composition and content of fatty acids, and the effects of different levels of Na-HCO3, NaCl and pH on an alkalophilic diatom strain, Nitzschia sp. MD1. We showed that MD1 contained 3.02% EPA in dry weight biomass, which was 17.93% of the total fatty acids. It was indicated that the optimal range of NaHCO3 for MD1 growth was between 0.1?0.2 M, and that MD1 showed a good growth even when NaHCO3 was below 0.3. The optimal range of initial pH for MD1 growth was between 7.5 and 9.5, and that MD1 maintained a considerable growth even when initial pH was 11.5. It was also shown that MD1 exhibited adaptation to a wide range of salinity, which brought about no obvious difference in the growth of MD1 when NaCl was between 0?40g•L?1. The maximum specific growth rate of MD1 reached 0.56d ?1 at 0.2mol•L?1 of NaHCO3, 30g•L?1 of NaCl, and 8.5?9.5 of pH. These results present a prospective future for large-scale cultivation and EPA production of MD1.

Key words: Nitzschia, alkalophilic, pH, NaHCO3, EPA.

苏娇娇,向文洲,萧邶,徐少琨,何慧. 菱形藻株系MD1的脂肪酸组成及其在NaHCO3、NaCl和pH值影响下的生长特征[J]. 热带海洋学报, 2010, 29(6): 79-85.

SU Jiao-jiao,XIANG Wen-zhou,XIAO Bei,XU Shao-kun,HE Hui. Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain[J]. Journal of Tropical Oceanography, 2010, 29(6): 79-85.

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菱形藻株系MD1的脂肪酸组成及其在NaHCO3、NaCl和pH值影响下的生长特征

Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain

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菱形藻株系MD1的脂肪酸组成及其在NaHCO3、NaCl和pH值影响下的生长特征

Effect of different levels of NaHCO3, NaCl and pH on the growth of a EPA-producing diatom strain

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Effect of different levels of NaHCO3, NaCl and pH on the growth of a
EPA-producing diatom strain