热带海洋学报 ›› 2010, Vol. 29 ›› Issue (5): 154-158.doi: 10.11978/j.issn.1009-5470.2010.05.154cstr: 32234.14.j.issn.1009-5470.2010.05.154

• 海洋生物学 • 上一篇    下一篇

温度和pH对条石鲷幼鱼消化酶活力的影响

罗奇1, 2, 区又君1, 艾丽1, 2, 李加儿1   

  1. 1. 中国水产科学研究院南海水产研究所, 中国水产科学研究院水产种质资源与养殖技术重点开放实验室, 广东 广州 510300; 2. 上海海洋大学水产与生命学院, 上海 201306
  • 收稿日期:2008-10-15 修回日期:2009-02-23 出版日期:2010-10-30 发布日期:2011-10-10
  • 通讯作者: 区又君。
  • 作者简介:罗奇(1986—), 男, 江苏省宿迁市人, 硕士研究生, 主要从事鱼类繁殖生物学研究。E-mail: luoqi198@163.com
  • 基金资助:

    中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放基金(2007A014)

Effects of temperature and pH on the digestive enzymes activities of juvenile striped beakperch Oplegnathus fasciatus

LUO Qi1,2 , OU You-jun1, AI Li1,2 , LI Jia-er1   

  1. 1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Key Laboratory of Fisheries Genetic Resources and Aquaculture, Chinese Academy of Fisheries Sciences; Guangzhou 510300, China; 2. College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China
  • Received:2008-10-15 Revised:2009-02-23 Online:2010-10-30 Published:2011-10-10
  • Contact: 区又君。
  • About author:罗奇(1986—), 男, 江苏省宿迁市人, 硕士研究生, 主要从事鱼类繁殖生物学研究。E-mail: luoqi198@163.com
  • Supported by:

    中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放基金(2007A014)

摘要:

研究了温度和pH对条石鲷Oplegnathus fasciatus幼鱼胃、肠、肝内3种主要消化酶(蛋白酶、淀粉酶和脂肪酶)活力的影响。结果表明, 1)肠和肝的蛋白酶最适温度均为25℃, 而胃蛋白酶最适温度为28℃; 胃和肝淀粉酶最适温度是22℃, 肠淀粉酶的最适温度为19℃; 各消化器官的脂肪酶在16℃时活力最高。当温度在16—28℃之间时, 条石鲷幼鱼的脂肪酶和蛋白酶活力较高, 淀粉酶活力较低。2)胃蛋白酶最适pH为3.2, 淀粉酶最适pH是7.6, 脂肪酶最适pH为4.2; 肠蛋白酶、淀粉酶和脂肪酶最适pH分别是8.6、6.6和6.6; 肝蛋白酶最适pH是7.6, 肝淀粉酶和脂肪酶最适pH均为6.6。

关键词: 条石鲷Oplegnathus fasciatus, 幼鱼, 温度, pH, 消化酶, 活力

Abstract:

The effects of temperature and pH on digestive enzymes activities (stomach, intestine and liver) were investigated in juvenile striped beakperch Oplegnathus fasciatus. The results indicated that the optimum temperature for protease in intes-tine and liver was 25℃, and that in stomach was 28℃. The optimum temperature for amylase in stomach and liver was 22℃, and that in intestine was 19℃. The optimum temperature for lipase in three digestive organs was all 16℃. During 16℃ to  28℃, the protease and lipase both had relatively high activities, however, the amylase had a lower activity. The optimum pH values for protease, amylase and lipase were 3.2, 7.6 and 4.2 in stomach, and 8.6, 6.6 and 6.6 in intestine, respectively. The optimum pH in liver was 7.6 for protease, and 6.6 for amylase and lipase.

Key words: striped beakperch Oplegnathus fasciatus, juvenile, temperature, pH, digestive enzymes, activity