海南三亚鹿回头虫黄藻(Effrenium voratum)的形态学和系统发育学研究*

doi:10.11978/2020102

热带海洋学报 ›› 2021, Vol. 40 ›› Issue (4): 35-43.doi: 10.11978/2020102CSTR: 32234.14.2020102

海南三亚鹿回头虫黄藻(Effrenium voratum)的形态学和系统发育学研究^*

龙超^1,^2,³(), 罗肇河⁴, 韦章良^1,^3,⁵, 杨芳芳^1,^3,⁵, 李茹¹, 龙丽娟^1,^3,⁵()

1.中国科学院热带海洋生物资源与生态重点实验室(中国科学院南海海洋研究所), 广东广州 510301
2.中国科学院大学, 北京 100049
3.南方海洋科学与工程广东省实验室(广州), 广东广州 511458
4.自然资源部第三海洋研究所, 福建厦门 361005
5.海南省热带海洋生物技术重点实验室(三亚中科海洋研究院), 海南三亚 572024

收稿日期:2020-09-10 修回日期:2020-11-05 出版日期:2021-07-10 发布日期:2020-11-16
通讯作者: 龙丽娟
作者简介:龙超(1984—), 男, 湖北省黄冈市人, 博士研究生, 研究方向为海洋生物学。email: longchao@scsio.ac.cn
基金资助:
南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0404);中国科学院战略性先导科技专项(A类)(XDA13020203)

Morphology and phylogeny of zooxanthellae Effrenium voratum from Luhuitou reef in Sanya, Hainan province

LONG Chao^1,^2,³(), LUO Zhaohe⁴, WEI Zhangliang^1,^3,⁵, YANG Fangfang^1,^3,⁵, LI Ru¹, LONG Lijuan^1,^3,⁵()

1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (South China Sea Institute of Oceanology, Chinese Academy of Sciences), Guangzhou 510301, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China
4. Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China
5. Hainan Provincial Key Laboratory of Tropical Marine Biology Technology (Sanya Institute of Oceanology Chinese Academy of Sciences), Sanya 572024, China

Received:2020-09-10 Revised:2020-11-05 Online:2021-07-10 Published:2020-11-16
Contact: LONG Lijuan
Supported by:
Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0404);Strategic Priority Research Program of the Chinese Academy of Sciences(XDA13020203)

摘要/Abstract

摘要：

本文对分离于我国海南三亚鹿回头海域的两株虫黄藻SYSC-14-11和SYSC-2-8进行了分类学研究。通过光学显微镜、扫描电子显微镜和分子生物学方法描述了两株藻的形态和系统发育特征, 并与世界其他地理区系的Effrenium属虫黄藻进行了差异性比较, 发现本研究中的两株虫黄藻的形态和系统发育特征与Effrenium属虫黄藻模式种Effrenium voratum基本一致, 推测本文中的两株Effrenium属虫黄藻均为E. voratum。本研究丰富了我国热带海域虫黄藻的物种多样性, 为完善我国的虫黄藻种质资源奠定了基础。

关键词: 虫黄藻, 共生藻科, Effrenium属, 形态学, 系统发育学

Abstract:

In this study, we analyzed taxonomy of two strains (SYSC-14-11 and SYSC-2-8) of zooxanthellae isolated from the Luhuitou reef region in Sanya. Morphological characteristics were determined by light microscope and scanning electron microscope, while phylogenetic analyses were constructed based on the sequences of the LSU rDNA and the internal transcribed spacer sequence (ITS2). The results showed that the morphological and phylogenetic characteristics of the two strains in the present study were basically consistent with Effrenium voratum reported in other geographical regions of the world. This study enriches the species diversity of zooxanthellae in the tropical coral reef ecosystems, and provides a solid foundation for further utilizing the resources of zooxanthellae.

Key words: Zooxanthellae, Symbiodiniaceae, Effrenium, morphology, phylogeny

龙超, 罗肇河, 韦章良, 杨芳芳, 李茹, 龙丽娟. 海南三亚鹿回头虫黄藻(Effrenium voratum)的形态学和系统发育学研究^*[J]. 热带海洋学报, 2021, 40(4): 35-43.

LONG Chao, LUO Zhaohe, WEI Zhangliang, YANG Fangfang, LI Ru, LONG Lijuan. Morphology and phylogeny of zooxanthellae Effrenium voratum from Luhuitou reef in Sanya, Hainan province[J]. Journal of Tropical Oceanography, 2021, 40(4): 35-43.

图/表 4

图1

图2

图3

图4

参考文献 37

[1]	张跃环, 肖述, 张扬, 等, 2018. 一种砗磲虫黄藻的分离纯化、离体培养及规模化生产方法: 中国, 201710979322.9[P] (in Chinese).
[2]	赵振鲁, 刘甲星, 张跃环, 等, 2019. 离体培养的虫黄藻(Symbiodinium voratum)对温度和光照的生理响应[J]. 海洋与湖沼, 50(2):316-323.
	ZHAO ZHENLU, LIU JIAXING, ZHANG YUEHUAN, et al, 2019. Physiological responses of Symbiodinium voratum to temperature and light intensity[J]. Oceanologia Et Limnologia Sinica, 50(2):316-323 (in Chinese with English abstract).
[3]	朱霞, 甄毓, 于志刚, 2011. 一株分离自胶州湾的裸甲藻形态相似种的分子系统学研究[J]. 海洋学报, 33(1):153-162.
	ZHU XIA, ZHEN YU, YU ZHIGANG, 2011. Molecular phylogenetic analysis of a Gymnodinium-like species isolated from the Jiaozhou Bay of Shandong Province, China[J]. Acta Oceanologica Sinica, 33(1):153-162 (in Chinese with English abstract).
[4]	BAKER A C, 2003. Flexibility and specificity in coral-algal symbiosis: diversity, ecology, and biogeography of Symbiodinium[J]. Annual Review of Ecology, Evolution, & Systematics, 34:661-689.
[5]	BERKELMANS R, VAN OPPEN M J H, 2006. The role of zooxanthellae in the thermal tolerance of corals: a ‘nugget of hope’ for coral reefs in an era of climate change[J]. Proceedings of the Royal Society B: Biological Sciences, 273(1599):2305-2312. doi: 10.1098/rspb.2006.3567
[6]	DOVER G, 1982. Molecular drive: a cohesive mode of species evolution[J]. Nature, 299(5879):111-117. doi: 10.1038/299111a0
[7]	FAN LIJING, SUI ZHENGHONG, MAO YUNXIANG, et al, 2007. Preliminary identification of three new isolates in genus Alexandrium (Dinophyceae) from China Sea area[J]. Journal of Ocean University of China, 6(1):33-39. doi: 10.1007/s11802-007-0033-6
[8]	FENSOME R A, TAYLOR F J R, NORRIS G, et al, 1993. A classification of living and fossil dinoflagellates[Z]. Micropaleontology Special Publication No.7.
[9]	FINNEY J C, PETTAY D T, SAMPAYO E M, et al, 2010. The relative significance of host-habitat, depth, and geography on the ecology, endemism, and speciation of coral endosymbionts in the genus Symbiodinium[J]. Microbial Ecology, 60:250-263. doi: 10.1007/s00248-010-9681-y
[10]	FISHER P L, MALME M K, DOVE S, 2012. The effect of temperature stress on coral-Symbiodinium associations containing distinct symbiont types[J]. Coral Reefs, 31:473-485. doi: 10.1007/s00338-011-0853-0
[11]	FURLA P, BÉNAZET-TAMBUTTÉ S, JAUBERT J, et al, 1998. Functional polarity of the tentacle of the sea anemone Anemonia viridis: role in inorganic carbon acquisition[J]. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 274(2):R303-R310.
[12]	GLYNN P W, 1993. Coral reef bleaching: Ecological perspectives[J]. Coral Reefs, 12(1):1-17. doi: 10.1007/BF00303779
[13]	GOU WANLI, SUN JUN, LI XIUQIN, et al, 2003. Phylogenetic analysis of a free-living strain of Symbiodinium isolated from Jiaozhou Bay, P. R. China[J]. Journal of Experimental Marine Biology & Ecology, 296(2):135-144.
[14]	HAYWOOD A J, STEIDINGER K A, TRUBY E W, et al, 2004. Coparative morphology and molecular phylogenetic analysis of three new species of the genus Karenia (Dinophyceae) from New Zealand[J]. Journal of Phycology, 40:165-179. doi: 10.1111/(ISSN)1529-8817
[15]	JEONG H J, YOO Y D, KANG N S, et al, 2012. Heterotrophic feeding as a newly identified survival strategy of the dinoflagellate Symbiodinium[J]. Proceedings of the National Academy of Sciences of the United States of America, 109(31):12604-12609.
[16]	JEONG H J, LEE S Y, KANG N S, et al, 2014. Genetics and morphology characterize the dinoflagellate Symbiodinium voratum, n. sp., (Dinophyceae) as the sole representative of Symbiodinium clade E[J]. Journal of Eukaryotic Microbiology, 61(1):75-94. doi: 10.1111/jeu.12088
[17]	JONES A M, BERKELMANS R, VAN OPPEN M J H, et al, 2008. A community change in the algal endosymbionts of a scleractinian coral following a natural bleaching event: field evidence of acclimatization[J]. Proceedings of the Royal Society B: Biological Sciences, 275(1641):1359-1365. doi: 10.1098/rspb.2008.0069
[18]	KIMURA M, 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences[J]. Journal of Molecular Evolution, 16:111-120. doi: 10.1007/BF01731581
[19]	LAJEUNESSE T C, TRENCH R K, 2000. Biogeography of two species of Symbiodinium (Freudenthal) inhabiting the intertidal sea anemone Anthopleura elegantissima (Brandt)[J]. Biological Bulletin, 199(2):126-134. doi: 10.2307/1542872
[20]	LAJEUNESSE T C, SMITH R T, FINNEY J, et al, 2009. Outbreak and persistence of opportunistic symbiotic dinoflagellates during the 2005 Caribbean mass coral “bleaching” event[J]. Proceedings of the Royal Society B: Biological Sciences, 276(1676):4139-4148. doi: 10.1098/rspb.2009.1405
[21]	LAJEUNESSE T C, SMITH R, WALTHER M, et al, 2010. Host-symbiont recombination versus natural selection in the response of coral-dinoflagellate symbioses to environmental disturbance[J]. Proceedings of the Royal Society B: Biological Sciences, 277(1696):2925-2934. doi: 10.1098/rspb.2010.0385
[22]	LAJEUNESSE T C, THORNHILL D J, 2011. Improved resolution of reef-coral endosymbiont (Symbiodinium) species diversity, ecology, and evolution through psbA non-coding[J]. PLoS One, 6(12):e29013. doi: 10.1371/journal.pone.0029013
[23]	LAJEUNESSE T C, PARKINSON J E, GABRIELSON P W, et al, 2018. Systematic revision of Symbiodiniaceae highlights the antiquity and diversity of coral endosymbionts[J]. Current Biology, 28(16):2570-2580.e6. doi: 10.1016/j.cub.2018.07.008
[24]	MULLER-PARKER G, LEE K W, COOK C B, 1996. Changes in the ultrastructure of symbiotic zooxanthellae (Symbiodinium sp., Dinophyceae) in fed and starved sea anemones maintained under high and low light[J]. Journal of Phycology, 32:987-994. doi: 10.1111/j.0022-3646.1996.00987.x
[25]	PARKINSON J E, COFFROTH M A, LAJEUNESSE T C, 2015. New species of Clade B Symbiodinium (Dinophyceae) from the greater Caribbean belong to different functional guilds: S. aenigmaticum sp. nov., S. antillogorgium sp. nov., S. endomadracis sp. nov., and S. pseudominutum sp. nov.[J]. Journal of Phycology, 51(5):850-858. doi: 10.1111/jpy.12340
[26]	POCHON X, GATES R D, 2010. A new Symbiodinium clade (Dinophyceae) from soritid foraminifera in Hawaiʹi[J]. Molecular Phylogenetics and Evolution, 56(1):492-497. doi: 10.1016/j.ympev.2010.03.040
[27]	RODRIGUEZ-LANETTY M., CHANG S J, SONG J I, 2003. Specificity of two temperate dinoflagellate-anthozoan associations from the north-western Pacific Ocean. Marine Biology, 143(6):1193-1199. doi: 10.1007/s00227-003-1165-x
[28]	ROWAN R, 1998. Review—Diversity and ecology of zooxanthellae on coral reefs[J]. Journal of Phycology, 34(3):407-417. doi: 10.1046/j.1529-8817.1998.340407.x
[29]	SAMPAYO E M, DOVE S, LAJEUNESSE T C, 2009. Cohesive molecular genetic data delineate species diversity in the dinoflagellate genus Symbiodinium[J]. Molecular Ecology, 18(3):500-519. doi: 10.1111/mec.2009.18.issue-3
[30]	SANTOS S R, 2004. Comment: phylogenetic analysis of a free-living strain of Symbiodinium isolated from Jiaozhou Bay, P.R. China[J]. Journal of Phycology, 40(2):395-397. doi: 10.1111/j.1529-8817.2004.03186.x
[31]	SHAO PENG, CHEN YUEQIN, ZHOU HUI, et al, 2004. Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis[J]. Marine Biology, 144:215-224. doi: 10.1007/s00227-003-1157-x
[32]	STERN R F, ANDERSEN R A, JAMESON I, et al, 2012. Evaluating the ribosomal internal transcribed spacer (ITS) as a candidate dinoflagellate barcode marker[J]. PLoS ONE, 7: e42780. doi: 10.1371/journal.pone.0042780
[33]	TRENCH R K, BLANK R J, 1987. Symbiodinium microadriaticum Freudenthal, S. goreauii sp. nov., S. kawagutii sp. nov. and S. pilosum sp. nov.: gymnodinioid dinoflagellate symbionts of marine invertebrates[J]. Journal of Phycology, 23(3): 469-481. doi: 10.1111/jpy.1987.23.issue-3
[34]	WHAM D C, NING GANG, LAJEUNESSE T C, 2017. Symbiodinium glynnii sp. nov., a species of stress-tolerant symbiotic dinoflagellates from pocilloporid and montiporid corals in the Pacific Ocean[J]. Phycologia, 56(4):396-409. doi: 10.2216/16-86.1
[35]	XIANG TINGTING, HAMBLETON E A, DENOFRIO J C, et al, 2013. Isolation of clonal axenic strains of the symbiotic dinoflagellate Symbiodinium and their growth and host specificity[J]. Journal of Phycology, 49(3):447-458. doi: 10.1111/jpy.12055
[36]	YAMASHITA H, KOIKE K, 2013. Genetic identity of free-living Symbiodinium obtained over a broad latitudinal range in the Japanese coast[J]. Phycological Research, 61(1):68-80. doi: 10.1111/pre.2013.61.issue-1
[37]	ZARDOYA R, COSTAS E, LÓPEZ-RODAS V, et al, 1995. Revised dinoflagellate phylogeny inferred from molecular analysis of large-subunit ribosomal RNA gene sequences[J]. Journal of Molecular Evolution, 41(5):637-645.

海南三亚鹿回头虫黄藻(Effrenium voratum)的形态学和系统发育学研究^*

Morphology and phylogeny of zooxanthellae Effrenium voratum from Luhuitou reef in Sanya, Hainan province

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 37

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	李能辉, 黄庆, 李航, 曾俊, 吴科锋, 谭华强. 基于形态和分子数据对湛江海域4个江蓠物种(红藻门)的分类学研究[J]. 热带海洋学报, 2024, 43(2): 34-47.
[2]	郁强, 董娴娴, 李长青, 张欣, 赵国理, CHIN Yaoxian, 王沛政. 除草剂乙草胺胁迫下两种软珊瑚生理响应[J]. 热带海洋学报, 2023, 42(4): 133-145.
[3]	黄思军, 邱晨, 龙超, 龙丽娟. 三亚湾珊瑚来源虫黄藻不同株系微环境中微生物群落结构的差异比较分析[J]. 热带海洋学报, 2022, 41(6): 90-104.
[4]	韩通, 李晶晶, 刘正一, 刘凯, 张金浩, 秦松, 钟志海. 红纤维虾形草(Phyllospadix iwatensis)种子形态及内部特征[J]. 热带海洋学报, 2022, 41(6): 105-113.
[5]	张喆, 俞晓磊, 梁宇娴, 何茜, 黄晖, 袁翔城, 周伟华. 海水升温对非虫黄藻共生型珊瑚Cladopsammia sp.的生理影响^*[J]. 热带海洋学报, 2022, 41(5): 141-149.
[6]	朱文涛, 夏景全, 刘相波, 尹洪洋, 朱铭, 任瑜潇, 谢敏睿, 黄建中, 李秀保. 丛生盔形珊瑚光合生理及共生真菌群落分析[J]. 热带海洋学报, 2022, 41(2): 132-141.
[7]	刘金梅, 黄冰心, 丁兰平, 王雪聪, 闫璟, 闫盼竹, 张瑶. 我国海南省东南部台湾那屿藻的形态观察及系统发育分析[J]. 热带海洋学报, 2021, 40(6): 76-82.
[8]	牛彪彪, 翟梦怡, 李扬. 塞舌尔角毛藻的形态学再描述和分子系统学分析[J]. 热带海洋学报, 2021, 40(4): 44-49.
[9]	刘金梅, 姜晶晶, 马鑫, 黄冰心, 杨楠, 刘美媛, 丁兰平. 我国粤东沙菜属(红藻门杉藻目)的分类鉴定 ^*[J]. 热带海洋学报, 2021, 40(1): 99-110.
[10]	黄丽芬, 李群, 吕颂辉, 张亮, 谢学东. 中国西沙群岛底栖甲藻热带库里亚藻(Coolia tropicalis)的形态学、系统发育及毒性研究[J]. 热带海洋学报, 2020, 39(3): 86-97.
[11]	江丽春, 李群, 吕颂辉. 广西涠洲岛底栖甲藻前沟藻属(Amphidinium)种类的形态学和系统发育学研究[J]. 热带海洋学报, 2020, 39(3): 106-115.
[12]	黄景, 潘肖兰, 许濛, 刘文广, 张华, 何毛贤. 形态学和SNP标记分析马氏珠母贝杂交子代及其亲本群体的遗传结构[J]. 热带海洋学报, 2019, 38(6): 80-89.
[13]	李永梅, 刘瑞, 杨楠, 丁兰平, 黄冰心, 王艺晓, 陈彦伟. 海南省4种江蓠属(红藻门)海藻的形态分类学研究[J]. 热带海洋学报, 2018, 37(4): 29-37.
[14]	朱凯, 王雪辉, 张鹏, 杜飞雁, 邱永松. 南海南部鸢乌贼中型群与微型群形态学差异及其判别分析^*[J]. 热带海洋学报, 2016, 35(6): 82-88.
[15]	沈城, 刘楚吾, 刘丽. 温度胁迫及恢复初期稀杯盔形珊瑚共生虫黄藻Hsp70、Hsp90、psaA、psbA基因表达分析[J]. 热带海洋学报, 2016, 35(3): 72-78.