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热带海洋学报 ›› 2025, Vol. 44 ›› Issue (1): 1-8.doi: 10.11978/2024049CSTR: 32234.14.2024049

• 海洋生物学 •    下一篇

南海小叶海蛞蝓(Phyllidiella nanhaiensis sp. nov.)线粒体基因组特征与系统进化

刘慧1,2(), 张辉贤2(), 刘馨蔓3, 林强2, 沈萍萍1()   

  1. 1.烟台大学海洋学院, 山东 烟台 264005
    2.中国科学院热带海洋生物资源与生态重点实验室, 广东省应用海洋生物重点实验室, 中国科学院南海海洋研究所, 广东 广州 510301
    3.珠海科技学院, 广东 珠海 519041
  • 收稿日期:2024-03-01 修回日期:2024-03-18 出版日期:2025-01-10 发布日期:2025-02-10
  • 通讯作者: 张辉贤, 沈萍萍
  • 作者简介:

    刘慧(1999—), 硕士研究生, 主要从事海洋动物行为生理学。email:

  • 基金资助:
    国家重点研发项目(2022YFC3102403); 国家重点研发项目(2021YFF0502803); 广东省应用海洋生物学重点实验室运行经费(2023B1212060047)

Complete mitogenome data of sea slug Phyllidiella nanhaiensis sp. nov. and its phylogenetic implications

LIU Hui1,2(), ZHANG Huixian2(), LIU Xinman3, LIN Qiang2, SHEN Pingping1()   

  1. 1. Ocean School, Yantai University, Yantai 264005, China
    2. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
    3. Zhuhai College of Science and Technology, Zhuhai 519041, China
  • Received:2024-03-01 Revised:2024-03-18 Online:2025-01-10 Published:2025-02-10
  • Contact: ZHANG Huixian, SHEN Pingping
  • Supported by:
    National Key Research and Development Program of China(2022YFC3102403); National Key Research and Development Program of China(2021YFF0502803); Science and Technology Planning Project of Guangdong Province, China(2023B1212060047)

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摘要：

南海小叶海蛞蝓(Phyllidiella nanhaiensis sp. nov.)发现于南海海域, 其形态特征与叶海牛科物种极为相似, 呈修长的卵圆形, 背部呈深灰色且具不规则乳白色瘤状突起, 其中央两排较大橙色突起并行分布。本文利用线粒体全基因组测序对其进行鉴定, 并根据蛋白编码基因(protein-coding genes, PCGs)序列分析其系统进化关系。结果表明, 该物种线粒体基因组全长为14677bp, 包含37个基因, 分别为13个PCGs、2个rRNA和22个tRNA。线粒体基因组的碱基组成为: A占30.4%、T占37.3%、C占15.0%、G占17.3%, A+T含量为67.7%。在13个PCGs中, 除了ND5和ND6使用TTG作为起始密码子外, 其他11个PCGs的起始密码子均符合ATN的形式。最大似然法进化树分析结果表明, 该物种与突丘小叶海蛞蝓(Phyllidiella pustulosa)亲缘关系极为密切, 并与媚眼叶海牛(Phyllidia ocellata)聚为一支。BLAST (basic local alignment search tool)分析发现, 该物种与P. pustulosa线粒体全基因组序列相似度为94.6%, COX1基因序列相似度为98.8%。结合形态特征与分子鉴定结果表明, 该物种为Phyllidiella属新种, 将之命名为南海小叶海蛞蝓(Phyllidiella nanhaiensis sp. nov.)。南海小叶海蛞蝓的发现及其线粒体基因组数据为Phyllidiella属的深入研究提供参考, 对海蛞蝓的物种多样性和系统发育研究具有重要意义。

关键词: 南海小叶海蛞蝓, 线粒体基因组, 进化分析, 物种鉴定

Abstract:

The sample was obtained in the South China Sea, and its morphological characters are very similar to those of the Phyllidiidae species. It is elongated and ovoid, with a dark grey dorsal surface and irregular creamy-white verrucae, and two rows of orange protrusions in the center of the dorsal surface in parallel. In this study, this species was identified by mitochondrial whole genome sequencing and analyzed based on the sequences of protein-coding genes (PCGs). The results show that the full length of the mitochondrial genome is 14677 bp, containing 37 genes, which are 13 PCGs, 2 rRNAs and 22 tRNAs. The base composition of the mitochondrial genome is 30.4% A, 37.3% T, 15.0% C, and 17.3% G, with a preference for AT (67.7%). Among the 13 PCGs, there are 11 start codons conformed to the codon form of ATN, which used TTG as the start codon. The evolutionary tree was constructed using the maximum likelihood method, and the results show that this species is closely related to Phyllidiella pustulosa and clustered with Phyllidia ocellata. The complete mitochondrial genome sequence similarity between this species and P. pustulosa reached 94.6% by BLAST, and the gene sequence similarity of COX1 reaches 98.8%. Based on combined morphological characters and molecular identification, it is a new species of the genus Phyllidiella in the South China Sea, Phyllidiella nanhaiensis sp. nov.. The mitogenome of P. nanhaiensis sp. nov. will provide a reference for further study of the genus Phyllidiella, which is important for the study of species diversity and phylogeny of sea slugs.

Key words: Phyllidiella nanhaiensis sp. nov., mitochondrial genome, phylogenetic analysis, species identification

中图分类号: 

  • P735