热带海洋学报 ›› 2021, Vol. 40 ›› Issue (5): 25-35.doi: 10.11978/2020119

• 海洋生物学 • 上一篇    下一篇

基于线粒体控制区序列的黄海日本鳀(Engraulis japonicus)的群体遗传结构*

邹聪聪1,2,3(), 王丽娟1,2, 吴志昊1,2, 尤锋1,2()   

  1. 1.中国科学院实验海洋生物学重点实验室, 海洋大科学中心(中国科学院海洋研究所), 山东 青岛 266071
    2.青岛海洋科学与技术试点国家实验室实验海洋生物学与生物技术实验室, 山东 青岛 266237
    3.中国科学院大学, 北京 100049
  • 收稿日期:2020-10-15 修回日期:2021-01-29 出版日期:2021-09-10 发布日期:2021-02-09
  • 通讯作者: 尤锋
  • 作者简介:邹聪聪(1994—), 女, 浙江省丽水市人, 博士研究生, 主要从事海水鱼类遗传学研究。email: zoucongcong17@mails.ucas.ac.cn
  • 基金资助:
    科技基础性工作专项(2014FY110500);国家海洋水产种质资源库项目(2017DKA30470)

Population genetic structure of Japanese anchovy (Engraulis japonicus) in the Yellow Sea based on mitochondrial control region sequences*

ZOU Congcong1,2,3(), WANG Lijuan1,2, WU Zhihao1,2, YOU Feng1,2()   

  1. 1. Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
    2. Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2020-10-15 Revised:2021-01-29 Online:2021-09-10 Published:2021-02-09
  • Contact: YOU Feng
  • Supported by:
    Special Program for Basic Research of the Ministry of Science and Technology of China(2014FY110500);National Marine Genetic Resource Center(2017DKA30470)

摘要:

遗传多样性和群体遗传结构是研究群体动态变化的重要内容, 也是种质资源评估与保护的基础。日本鳀(Engraulis japonicus)是我国东部近海的重要鱼种, 具有重要的生态价值和经济价值。文章利用线粒体控制区全序列分析了黄海海域4个地理群体(北黄海北部、北黄海南部、南黄海北部和南黄海中部)日本鳀的遗传多样性、种群遗传结构和历史动态变化。结果显示, 131个样品检测到了126个单倍型, 且4个群体的单倍型多样性均很高, 其中北黄海南部群体的最低(0.995±0.009), 南黄海中部群体的最高(1.000±0.014)。而核苷酸多样性均较低, 为0.010±0.005 ~ 0.011±0.006。主成分判别分析(DAPC)和遗传分化系数Fst表明4个群体无明显的群体分化现象, 群体间的遗传同质性水平高, 分子方差分析(AMOVA)也显示分子变异基本来自于群体内, 并且没有明显的群体遗传结构。贝叶斯系统发育树分析发现, 黄海日本鳀有2个谱系, 谱系1和谱系2的分化时间为0.701Ma前, 可以追溯到更新世期间; 进一步中性检验和核苷酸错配分布分析表明这2个谱系可能发生过群体扩张。贝叶斯天际线图则显示黄海鳀鱼的有效群体数量在0.150Ma前发生了明显的下降。

关键词: 黄海, 日本鳀, 遗传多样性, 群体遗传结构, 更新世

Abstract:

Genetic diversity and population structure are important aspects in population dynamics study, and are the basis of fish resources evaluation and protection. Japanese anchovy (Engraulis japonicus) is an important fish species in the China East Coast, which has both ecological and economic values. In this study, the whole sequence of mitochondrial control region was used to analyze the genetic diversity, population genetic structure, and demographic history of its four geographical populations in the Yellow Sea, namely, north of the northern Yellow Sea (NYN), south of the northern Yellow Sea (NYS), north of the southern Yellow Sea (SYN), and central part of the southern Yellow Sea (SYC). A total of 126 haplotypes were detected in 131 samples of the four populations, and their haplotype diversity levels were high, among which the lowest was in the NYS (0.995±0.009) and the highest was in the SYC (1.000±0.014). In all populations, the nucleotide diversity was low (0.010±0.005 to 0.011±0.006). The results of Discriminant Analysis of Principal Components (DAPC) and Fst values indicated that there was no significant segregation among the four populations, and the level of genetic homogeneity among populations was high. There was no obvious population genetic structure, with the large molecular variation within the populations based on the Analysis of Molecular Variance (AMOVA). The Bayesian phylogenetic tree revealed two distinct lineages, which diverged at 0.701 million years ago, dating back to the Pleistocene period. Neutrality test and nucleotide mismatch distribution analyses demonstrated that these two lineages experienced demographic expansions. The effective population of Japanese anchovy in the Yellow Sea decreased significantly at 0.150 million years ago by analyzing the Bayesian skyline.

Key words: Yellow Sea, Engraulis japonicus, genetic diversity, population genetic structure, Pleistocene

中图分类号: 

  • P735.54