基于mtDNA控制区序列的3个黑棘鲷群体遗传结构特性研究

doi:10.11978/j.issn.1009-5470.2015.01.008

热带海洋学报 ›› 2015, Vol. 34 ›› Issue (1): 56-63.doi: 10.11978/j.issn.1009-5470.2015.01.008CSTR: 32234.14.j.issn.1009-5470.2015.01.008

基于mtDNA控制区序列的3个黑棘鲷群体遗传结构特性研究

施晓峰^{1, 2}, 苏永全¹, 王文成¹, 王军¹

1. 厦门大学海洋与地球学院, 福建厦门 361005;
2. 国家海洋局第三海洋研究所, 福建厦门 361005

收稿日期:2013-10-27 修回日期:2014-05-04 出版日期:2015-02-10 发布日期:2015-02-10
作者简介:施晓峰(1986~), 男, 博士, 福建省晋江市人, 助理研究员, 博士, 从事海洋生物化学与分子生物学研究。E-mail: xiaofengshi@xmu.edu.cn
基金资助:
海洋公益性行业科研专项项目(201005013、201105012)

Population genetic structure of three stocks of Acanthopagrus schlegelii based on mtDNA control region sequences

SHI Xiao-feng, SU Yong-quan, WANG Wen-cheng, WANG Jun

1. College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China;
2. Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received:2013-10-27 Revised:2014-05-04 Online:2015-02-10 Published:2015-02-10
Contact: 王军。E-mail: junw@xmu.edu.cn

摘要/Abstract

摘要： 本研究通过控制区序列片段, 分析来源于浙江东极(DJ)、广东澳头(AT)及海南万宁(WN)海域3个黑棘鲷Acanthopagrus schlegelii野生群体间的遗传多样性、遗传结构及群体历史动态。D-loop基因序列测定结果表明, 3个群体88个个体长序列长度范围为715~716bp, 共定义了59个单倍型, 单倍型多样性0.949~0.985, 核苷酸多样性0.0068~ 0.00901, 表明选取的3个野生群体都具有较高的遗传多样性水平; 由AMOVA分析结果可以看出, 东极与澳头2个群体的遗传分化较为明显, 其余群体间则无明显的遗传分化; 构建的单倍型邻接关系树显示出3个群体未有明显的谱系结构, 从而支持了可将黑棘鲷南方群体归为同一个管理单元的观点; 中性检验及核苷酸不配对分布表明黑棘鲷经历过更新世晚期的群体扩张事件。地理历史事件、海流及人工增殖放流的影响可能是黑棘鲷各群体现有的遗传多样性和遗传结构的主要原因。

关键词: 黑棘鲷, 遗传多样性, 遗传结构特性, 线粒体, 控制区

Abstract: In order to elucidate background information on the genetic diversity and population history of Acanthopagrus schlegelii, the genetic diversity, population genetic structure and demographic history of Dongji stock, Aotou stock and Wanning stock of the black porgy were analyzed based on the control region fragment of mitochondrial DNA in this study. The results showed that the sequences of 88 individuals of the three stocks were 715~716 bp in length and that there were 59 haplotypes. The value of haplotype diversity ranged from 0.949 to 0.985 and the nucleotide diversity, from 0.0068 to 0.00901. These results indicated that the three wild stocks presented a high level of genetic diversity. Significant population differentiation was detected for the two populations of Dongji stock and Aotou stock, while there was no significant genetic differentiation between Dongji stock and Wanning stock as well as between Aotou stock and Wanning stock. In addition, no obvious lineages and geographic clusters were found in the neighbor-joining tree, which supported the notion that unique management unit (MU) was recognized on the basis of the southern populations. Both neutrality tests and mismatch distribution analysis suggested a late Pleistocene population expansion for the three stocks. The genetic diversity and population genetic structure revealed were attributed to the absence of geographic barrier, influence of sea currents and human activities.

Key words: Acanthopagrus schlegelii, genetic diversity, genetic structure, mitochondrion, control region

施晓峰, 苏永全, 王文成, 王军. 基于mtDNA控制区序列的3个黑棘鲷群体遗传结构特性研究[J]. 热带海洋学报, 2015, 34(1): 56-63.

SHI Xiao-feng^{1, 2}, SU Yong-quan¹, WANG Wen-cheng¹, WANG Jun¹. Population genetic structure of three stocks of Acanthopagrus schlegelii based on mtDNA control region sequences[J]. Journal of Tropical Oceanography, 2015, 34(1): 56-63.

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基于mtDNA控制区序列的3个黑棘鲷群体遗传结构特性研究

Population genetic structure of three stocks of Acanthopagrus schlegelii based on mtDNA control region sequences

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