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热带海洋学报 ›› 2013, Vol. 32 ›› Issue (3): 47-51.doi: 10.11978/j.issn.1009-5470.2013.03.007cstr: 32234.14.j.issn.1009-5470.2013.03.007

• 海洋生物学 • 上一篇    下一篇

18株南海海洋真菌的初步鉴定及其发酵产物的细胞毒活性和抗菌活性筛选

张玲1, 2, 3, 张庆波1, 3, 陈玉婵2, 李浩华2, 王磊2, 章卫民2   

  1. 1. 中国科学院南海海洋研究所, 广东 广州 510301; 2. 广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 省部共建华南应用微生物国家重点实验室, 广东 广州 510070; 3. 中国科学院大学, 北京 100049
  • 收稿日期:2011-12-22 修回日期:2012-02-20 出版日期:2013-08-27 发布日期:2013-08-28
  • 通讯作者: 国家自然科学基金项目(31272087)
  • 作者简介:张玲(1984—), 女, 山东省临沂市人, 在读硕士, 研究方向为微生物活性代谢产物。电话: 020-87688612; E-mail: zhangling2006-22@163.com

Preliminary identification of 18 marine fungal strains from the South China Sea and screening of their fermentation products for cytotoxic and antibacterial activities

ZHANG Ling1, 2, 3, ZHANG Qing-bo1, 3, CHEN Yu-chan2, LI Hao-hua2, WANG Lei2, ZHANG Wei-min2   

  1. 1. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 2. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Collection and Application, Guangdong Provincial Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangzhou 510070, China; 3. University of Chinese Academy of Sciences, Beijing 100049, China;
  • Received:2011-12-22 Revised:2012-02-20 Online:2013-08-27 Published:2013-08-28
  • Contact: 章卫民。电话: 020-37656321; E-mail:wmzhang58@qq.com

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被引次数

10 | 2

摘要：

从南海海洋沉积物中分离得到18株真菌, 经内转录间隔区(ITS)序列测定进行初步鉴定; 采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法对其发酵液及菌丝体的提取物进行体外细胞毒活性和抗菌活性筛选。结果显示, 对人乳腺癌细胞株MCF-7增殖的抑制率在90%以上的菌株有5株; 对金黄色葡萄球菌的抑制率在90%以上的有15株, 占分离菌株总数的83.3%; 对大肠杆菌和铜绿假单胞菌的抑制率在90%以上的各有2株, 其中有1株对以上3种供试细菌都表现出较强的抑制活性, 抑制率达90%以上。

关键词: 海洋真菌, 细胞毒, 抗菌

Abstract:

Eighteen strains of marine fungi were isolated from the sediments of the South China Sea and primarily identified by intergenic transcribed spacer (ITS) sequencing. Cytotoxic and antibacterial activities of their fermentation products were evaluated using the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) method. The results showed that five strains displayed cytotoxic activity against human breast adenocarcinoma cell line (MCF-7) with an inhibition rate of above 90%; fifteen strains exhibited bacterial inhibition against Staphylococcus aureusat the rate above 90%, accounting for 83.3% of the total strains isolated; two strains showed bacterial inhibition against Escherichia coliat the rate above 90% and two strains showed bacterial inhibition against Pseudomonas aeruginosaat the rate above 90%; and one strain was found to show strong bacterial inhibition against the three tested bacteria.

Key words: marine fungi, cytotoxic, antibacterial

中图分类号: 

  • P735.51