海南岛海洋绿藻新记录种未氏仙掌藻(Halimeda velasquezii)的形态学与分子系统学分析

doi:10.11978/2020021

热带海洋学报 ›› 2020, Vol. 39 ›› Issue (6): 114-121.doi: 10.11978/2020021CSTR: 32234.14.2020021

海南岛海洋绿藻新记录种未氏仙掌藻(Halimeda velasquezii)的形态学与分子系统学分析

孟天¹(), 陈佐¹, 朱军^1,², 邹潇潇^1,², 符青艳¹, 鲍时翔^1,²()

1.中国热带农业科学院热带生物技术研究所, 海南海口 571101
2.海南省海洋生物资源功能性成分研究与利用重点实验室, 海南海口 571101

收稿日期:2020-02-24 修回日期:2020-05-14 出版日期:2020-10-10 发布日期:2020-06-22
通讯作者: 鲍时翔
作者简介:孟天(1990—), 女, 山东省淄博市人, 硕士, 大型海藻生物学。email: 2009074mt@163.com
基金资助:
农业农村部财政专项项目(NFZX2018);中央级公益性科研院所基金(1630052019010);中央级公益性科研院所基金(1630052020016)

Morphological and molecular study on marine green alga, Halimeda velasquezii, first recorded in Hainan Island

MENG Tian¹(), CHEN Zuo¹, ZHU Jun^1,², ZOU Xiaoxiao^1,², FU Qingyan¹, BAO Shixiang^1,²()

1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 570000, China
2. Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, China

Received:2020-02-24 Revised:2020-05-14 Online:2020-10-10 Published:2020-06-22
Contact: BAO Shixiang
Supported by:
Financial Fund of the Ministry of Agriculture and Rural Affairs of China(NFZX2018);National Non-profit Institute Research Grant of CATAS-ITBB from Chinese Government(1630052019010);National Non-profit Institute Research Grant of CATAS-ITBB from Chinese Government(1630052020016)

摘要/Abstract

摘要：

2019年4月在海南岛冯家湾潮间带的野外调查过程中采集到一种仙掌藻, 利用传统形态分类学与分子系统分类学结合的方法, 将该种鉴定为未氏仙掌藻Halimeda velasquezii W.R. Taylor。该种的形态特征为藻体呈绿色钙化, 直立生长, 固着器单一较小, 由横卵形或肾形节片连接形成, 节片表面观形态为近圆形, 内部为管状髓丝, 节点处髓丝多发生侧面成对融合, 皮层下髓丝末端无囊状膨大。1,5-二磷酸核酮羧化酶/氧化酶大亚基基因(ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, rbcL)的序列分析结果显示, 在系统发育树上, 本研究样本与印度洋-太平洋地区产的未氏仙掌藻处于同一分支, 且自展支持值为100, 碱基基因差异为9bp (0.69%)。该种在海南岛海域首次被发现, 为新记录种。本研究同时首次提取并分析了中国种群的rbcL基因序列, 丰富了该种的中国种群的生态学知识, 扩展了其地理分布范围, 对了解海南岛海域海藻资源的生物多样性及其分布特征具有重要意义。

关键词: 未氏仙掌藻, 海南岛, 分类, 形态, rbcL基因

Abstract:

Samples of Halimeda were collected from the intertidal zone of Fengjia Bay in Hainan Island during the field survey in April 2019. Detailed morphological structure observation and molecular systematics analysis were conducted. The results showed that the sample was identified as Halimeda velasquezii W.R. Taylor. We found the following combination of the characters: the thalli is green, calcified, erect from a single small stupose holdfast; segments are transversely oval to reniform linking together; utricles form in surface view is round or a little compressed; filaments inside are long tubular; medullary filaments fuse for a short distance in pairs in common; subcortical filaments loosely branch, and are not utriculiform. The rbcL gene analysis showed that the sample was clustered on the same branch as that from the Indian Ocean-Pacific Ocean region, whose bootstrap value and sequence divergence were 100 and 9 bp (0.69%), respectively. The species was the first recorded in Hainan Island. The results should enrich our knowledge of Chinese species’ morphology and extend their geographical distribution. They should be of great significance to understand the biodiversity and distribution characteristics of algal resources in the waters of Hainan Island.

Key words: Halimeda velasquezii, Hainan Island, classification, morphology, rbcL gene

中图分类号:

P735.121

孟天, 陈佐, 朱军, 邹潇潇, 符青艳, 鲍时翔. 海南岛海洋绿藻新记录种未氏仙掌藻(Halimeda velasquezii)的形态学与分子系统学分析[J]. 热带海洋学报, 2020, 39(6): 114-121.

MENG Tian, CHEN Zuo, ZHU Jun, ZOU Xiaoxiao, FU Qingyan, BAO Shixiang. Morphological and molecular study on marine green alga, Halimeda velasquezii, first recorded in Hainan Island[J]. Journal of Tropical Oceanography, 2020, 39(6): 114-121.

图/表 4

表1

图1

图2

表2

本研究样本与已报道的属内形态相似种的特征比较(Dijoux et al, 2012)"

样本与物种	形态特征
样本与物种	藻体形状	节片形状	节片尺寸	固着器	节点融合	囊胞表面观
QM190425	直立	全缘, 横卵形或肾形	长(4~10mm) <宽(5~15mm)	单一, 成簇细丝状	2~5根丝体局部侧面融合	圆形或椭圆形
H. velasquezii	直立或下垂	边缘平整, 宽椭圆形或肾形	长(2~7mm) <宽(3~10mm)	单一, 丝状假根束	2~3根丝体局部融合	圆形或多边形
H. discoidea	匍匐生长	边缘平整, 肾形或三叶形, 常有中肋	长(4~8mm) <宽(6~20mm)	多个分散的假根固着器	2~5根丝体局部侧面融合	多边形
H. opuntia	匍匐生长, 形成矮密藻丛	边缘平整, 肾形, 有时有中肋	长(3~7mm) <宽(4~8mm)	多个分散的假根固着器	2根局部融合, 很少有3根融合	多边形, 有时圆形
H. macroloba	直立	边缘平整, 盘形或肾形	长(8~20mm) <宽(14~30mm)	球根状	融合在一起, 中间有气孔	圆形或多边形
H. gracilis	匍匐生长, 分枝稀疏	边缘平整, 三叶形、宽卵形或窄楔形	长(2~6mm)=宽(1~6mm)	多个分散的假根固着器	2根(很少3根 ) 完全融合	多边形, 有时圆形
H. heteromorpha	直立, 丛生	边缘平整, 三叶形、肾形	长(3~8mm) >宽(1~10mm)	单一, 丝状假根束	融合在一起, 中间无气孔	多边形
H. cylindracea	直立	圆柱状或三叶状	长(1~9mm)=宽(1~8mm)	球根状	融合在一起, 中间有气孔	圆形或多边形
H. borneensis	直立	边缘平整, 三叶形或盘形	长(4~11mm)=宽(4~14mm)	球根状	融合在一起, 中间有气孔	圆形或多边形
H. kanaloana	直立	边缘平整, 宽三叶形或扇形	长(12mm) <宽(15~20mm)	球根状	融合在一起, 中间有气孔	多边形
H. melanesica	直立	边缘平整, 三叶形或楔形	长(3~5mm)=宽(4~5mm)	单一, 丝状假根束	融合在一起, 中间有小气孔	多边形

表2

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物种	采集地点	rbcL序列登录号
Halimeda velasquezii Taylor	印度洋-太平洋地区	FJ624507.1
H. borneensis W.R.Taylor	澳大利亚新厄里多尼亚岛	AB619273.1
H. borneensis W.R.Taylor	澳大利亚新厄里多尼亚岛	AB700353.1
H. cylindracea Decaisne	印度洋-太平洋地区	FJ624510.1
H. gracilis Harvey ex J.Agardh	大西洋地区	FJ624493.1
H. heteromorpha N'Yeurt	印度洋-太平洋地区	FJ624511.1
H. kanaloana Vroom	印度洋-太平洋地区	FJ624512.1
H. macroloba Decaisne	印度洋-太平洋地区	FJ624513.1
H. melanesica Valet	印度洋-太平洋地区	FJ624515.1
Udotea flabellum (J.Ellis & Solander) M.Howe	加勒比海地区	MG784872.1
U. argentea Zanardini	澳大利亚	NC_039527.1:45455-46882
Boodleopsis pusilla (Collins) W.R.Taylor, A.B.Joly & Bernatowicz	澳大利亚	NC_039520.1:24118-25545
Chlorodesmis sp. Tenija_051113A	日本冲绳	AB619306.1
Chlorodesmis sp. Tenija_051113A	日本冲绳	AB619308.1
Chlorodesmis sp. Tenija_050807A	日本冲绳	AB619311.1
Caulerpa racemosa (Forssk?l) J.Agardh	日本Tohwa-cho	AB038486.1
C. subserrata Okamura	日本神户	AB054015.1
C. taxifolia (M.Vahl) C.Agardh	日本神户	AB054016.1

海南岛海洋绿藻新记录种未氏仙掌藻(Halimeda velasquezii)的形态学与分子系统学分析

Morphological and molecular study on marine green alga, Halimeda velasquezii, first recorded in Hainan Island

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