罗氏沼虾线粒体锰超氧化物歧化酶的克隆表达与功能研究

doi:10.11978/2021109

Abstract

Abstract:

Macrobrachium rosenbergii is one of the important shrimps for consumers in China. In recent years, bacterial diseases have occurred frequently and caused huge economic losses in M. rosenbergii aquaculture. Therefore, understanding its immune mechanism is essential to guide disease prevention and control. Mitochondrial manganese superoxide dismutase (mMnSOD) is considered to be the first line against oxidative stress, playing a vital role in innate immunity. However, the immune function of mMnSOD is still unclear. To this end, we cloned the mMnSOD of M. rosenbergii (MrmMnSOD), prepared its polyclonal antibodies, and analyzed its expression patterns under Aeromonas hydrophila infection. On the gene expression levels, MrmMnSOD reached a peak after 3- and 6-h infection in hepatopancreas and intestine tissue, respectively. Tissue immunofluorescence analysis showed that the maximum fluorescence intensity occurred at 12 h after infections in both hepatopancreas and intestine tissue. The above results indicated that MrmMnSOD is involved in the immune response against A. hydrophila. Further antibacterial assays showed that MrmMnSOD significantly inhibited the growth of Escherichia coli, A. hydrophila, Vibrio parahaemolyticus, Staphylococcus aureus, and Streptococcus agalactiae, suggesting that MrmMnSOD may belong to an immune-related molecule, which exerts immune function by antibacterial effect. These results enrich the basic theory in crustacean innate immunity, and provide a new target for disease control and drug research for M. rosenbergii breeding in the future.

Key words: Macrobrachium rosenbergii, SOD, expression pattern, antibacterial assay

CLC Number:

S945.41

LI Yanan, LU Linqing, ZHANG Peng, QIN Zhendong, LIN Li, YAN Lei. Molecular cloning and functional study of mitochondrial manganese superoxide dismutase in Macrobrachium rosenbergii[J].Journal of Tropical Oceanography, 2022, 41(3): 111-118.

Figures/Tables 7

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References 31

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引物	序列 (5′—3′)	用途
MrmMnSOD S1	CCCTGAAGTTCAATGGAGGAGGT	qRT-PCR
MrmMnSOD A1	TTGGGACAAGTAGCAATTTGTAGTG	qRT-PCR
Mr18S F	TAGCAATTCGCCGTCGTTATTC	qRT-PCR内参
Mr18S R	CTACCCCCGGAACTCAAAGACT
MrmMnSOD-32a S	TATTTTCAGGGATCCGAATTCATGCTCAGTTTCAGCCGTCTG
MrmMnSOD-32a A	GTGGTGGTGGTGGTGCTCGAGTCACTTTGCAGCAGCAAATCTT	载体构建

Molecular cloning and functional study of mitochondrial manganese superoxide dismutase in Macrobrachium rosenbergii

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