基于线粒体DNA细胞色素b序列分析3个条纹斑竹鲨群体的遗传结构和遗传分化

doi:10.11978/j.issn.1009-5470.2010.06.086

Journal of Tropical Oceanography ›› 2010, Vol. 29 ›› Issue (6): 86-91.doi: 10.11978/j.issn.1009-5470.2010.06.086cstr: 32234.14.j.issn.1009-5470.2010.06.086

• Marine biology • Previous Articles Next Articles

Studies on the genetic structure and genetic subdivision of white spotted bamboo shark, Chiloscyllium plagiosum, by analysing mitochondrial Cyt b genes

FU Meng-na, WANG Jun, DING Shao-xiong, DU Jia-ying, SU Yong-quan

College of Ocean and Enviroment, Xiamen University, Xiamen 361005, China

Received:2008-12-26 Revised:2009-07-17 Online:2010-12-15 Published:2010-12-15
Contact: 苏永全。 E-mail:yqsu@xmu.edu.cn
About author:傅蒙娜(1983—), 女, 山东省即墨市人, 硕士研究生, 从事海洋鱼类群体遗传学研究。E-mail: fmn_1126@163.com
Supported by:
国家自然科学基金项目(40776083、40876080); 厦门市科技项目(3502Z20063022)

Abstract

Abstract:

The genetic diversity and genetic structure of Chiloscyllium plagiosum were assessed from three populations (Hainan, Guangdong, and Fujian), using mitochondrial Cyt b sequences. The complete mitochondrial Cyt b gene sequences (1146 nucleotides) from 71 individuals were defined as 15 haplotypes by 15 polymorphic sites after sequence alignment. The low levels of nucleotide diversity (π=0.00254) and haplotype diversity (h = 0.7195)indicated slow rate of mitochondrial DNA evolution in C. plagiosum . The result of selective neutrality tests (Tajima’s D, P>0.10) suggested that there was no population expansion of the C. plagiosum in these three areas and the sizes of the three populations were relatively stable. The phyloge-netic tree and the network based on haplotype indicated that the population in Guangdong was the ancestor, while the group in Fujian appeared recently. The results of genetic distance and gene flow analysis and the results of AMOVA revealed distinct genetic differentiations between Hainan and Fujian groups and between Guangdong and Fujian groups, while the genetic dif-ferentiation is not significant between Hainan and Guangdong groups. Our research suggested that population differentiation occurred as a consequence of isolation-by-distance.

Key words: Chiloscyllium plagiosum, mitochondrion, cytochrome b, population genetics

FU Meng-na,WANG Jun,DING Shao-xiong,DU Jia-ying,SU Yong-quan. Studies on the genetic structure and genetic subdivision of white spotted bamboo shark, Chiloscyllium plagiosum, by analysing mitochondrial Cyt b genes[J].Journal of Tropical Oceanography, 2010, 29(6): 86-91.

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Studies on the genetic structure and genetic subdivision of white spotted bamboo shark, Chiloscyllium plagiosum, by analysing mitochondrial Cyt b genes

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