高效溶藻放线菌BS01发酵培养基及发酵条件优化

doi:10.11978/j.issn.1009-5470.2011.02.109

Journal of Tropical Oceanography ›› 2011, Vol. 30 ›› Issue (2): 109-114.doi: 10.11978/j.issn.1009-5470.2011.02.109cstr: 32234.14.j.issn.1009-5470.2011.02.109

• Marine biology • Previous Articles Next Articles

Optimization of medium components and culture conditions of algicidal actinomy-cetes BS01

FU Li-jun1,2, AN Xin-li1,3, LI Dong2, XU Li-xia2, TIAN Yun1,3, ZHENG Tian-ling1,3

1. Key Laboratory of Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China; 2. Department of Environment and Life Science, Putian University, Putian 351100, China; 3. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China

Received:2010-07-26 Revised:2010-09-15 Online:2011-05-06 Published:2011-05-04
Contact: 郑天凌。E-mail: microzh@xmu.edu.cn E-mail:microzh@xmu.edu.cn
About author:傅丽君(1975—), 女, 福建省莆田市人, 博士, 副教授, 主要从事环境生态学研究。
Supported by:
国家高技术研究发展计划项目(2008AA09Z408); 国家自然科学基金项目(40930847、40876061); 滨海湿地生态系统教育部重点实验室开放基金项目(CWe0902); 福建省自然科学基金项目(2010J01223); 福建省教育厅项目(JA10232)

Abstract

Abstract:

A strain of actinomycetes BS01 capable of lysing the toxic dinoflagellate Alexandrium tamarense was isolated from mangrove area of Zhangjiangkou in Fujian province. The medium components and cultural conditions for algicidal activity of actinomycete strain BS01 were optimized by single-factor and uniform tests in shake-flask. The initial pH, fer-mentation temperature, fermentation time, and inoculum were optimized through single-factor tests. Results showed that BS01 exhibited the best algicidal activity by using soluble starch as carbon source, sodium nitrate as nitrogen source, initial pH at 7.7, temperature at 27.2℃, and shaking at 150r•min-1 in 100mL of 250mL conical flask for 48h. The optimal fer-mentation parameters of BS01 were: soluble starch 20g•L-1, sodium nitrate 0.5g•L-1, pH value 7.7, and temperature 27.2℃ by using uniform design experiments.

Key words: algicidal actinomycete, Alexandrium tamarense, optimization of medium component, single-factor experiment, uniform design

FU Li-jun,AN Xin-li,LI Dong,XU Li-xia,TIAN Yun,ZHENG Tian-ling,. Optimization of medium components and culture conditions of algicidal actinomy-cetes BS01[J].Journal of Tropical Oceanography, 2011, 30(2): 109-114.

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Optimization of medium components and culture conditions of algicidal actinomy-cetes BS01

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