生长激素与胰岛素样生长因子 - Ⅰ对马氏珠母贝壳生长形成相关基因表达的影响

doi:10.11978/j.issn.1009-5470.2012.02.013

Abstract

Abstract: This study investigated the expression levels of insulin-related peptide receptor gene ( irr) and several shell matrix protein genes ( nacrein, efcbp, n19, aspein, and accbp) in human recombinant growth hormone injection and insulin-like growth factor injection groups compared to the reference group in pearl oyster Pinctada fucata Gould. The expression level of irr was increased by hormone treatments ( P<0.05), which is consistent with its role as insulin-like growth factor receptor in mollusk. Higher transcripts of nacrein indicated higher growth metabolism levels in the pearl oysters of the experimental groups; compared to the reference group, the expression levels of n19, aspein and accbp decreased after growth hormone (GH) and insulin-like growth factor- Ⅰ (IGF- Ⅰ ) treatments ( P<0.05), suggesting they were suppressed by the GH and IGF- Ⅰ pathways. In addition, the expression levels of aspein and accbp were found to be highly correlated; we infer that these two genes may be regulated by a single upstream factor. The expression level of efcbp showed a relatively steady expression in all oysters, and no significant differences were found among these groups ( P>0.05).

Key words: growth hormone, insulin-like growth factor- Ⅰ, insulin-related peptide receptor, shell matrix protein, gene expression, Pinctada fucata Gould

CLC Number:

Q959.215

ZHANG Li-juan,HE Mao-xian. Effects of growth hormone and insulin-like growth factor- Ⅰ on shell growth related gene expression in Pinctada fucata Gould[J].Journal of Tropical Oceanography, 2012, 31(2): 96-101.

References

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Effects of growth hormone and insulin-like growth factor- Ⅰ on shell growth related gene expression in Pinctada fucata Gould

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