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Journal of Tropical Oceanography ›› 2012, Vol. 31 ›› Issue (4): 117-123.doi: 10.11978/j.issn.1009-5470.2012.04.016 cstr: 32234.14.j.issn.1009-5470.2012.04.016

• Marine geomorphology • Previous Articles     Next Articles

Effects of nitrate and phosphate on the growth and toxin production of Amphidinium klebsii (Sanya strain)

LONG Chao1,2, LONG Li-juan1   

  1. 1. Guangdong Key Laboratory of Marine Material, Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 
    2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-02-19 Revised:2011-04-17 Online:2012-10-22 Published:2012-10-24

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Abstract:

In order to evaluate the growth and toxin production of Amphidinium klebsii (Sanya strain) under different nitrate and phosphate concentrations, a series of nitrate and phosphate solutions were prepared based on modified K medium. Nitrate and phosphate had significant effects on the growth and toxin productivity of A. klebsii. Low-nitrate and low-phosphate concentrations (N concentration≤4.665×10-4mol•L-1, P concentration≤2.000×10-5mol•L-1) in culture media were not favorable to the biomass accumulation of A. klebsii but favorable to its growth and toxin production. On the contrary, middle-nitrate and middle-phosphate concentrations (4.665×10-4mol•L-1-4mol•L-1, 2.000×10-5mol•L-1-5mol•L-1) were favorable to the biomass accumulation of A. klebsii but not favorable to its toxin production. Extra-high-level nitrate and phosphate concentrations (N concentration≥37.320×10-4mol•L-1, P concentration≥16.000×10-5mol•L-1) seemed to inhibit the growth of A. klebsii, but resulted in higher cell densities and contributed to its toxin production. Under the experimental condition, optimum growth N and P concentrations were 4.665×10-4mol•L-1-4mol•L-1, 2.000×10-5mol•L-1-5mol•L-1. The highest biomass value estimated in culture was 1.237×105mL-1 at the levels of nitrate 9.330×10–4mol•L–1 and phosphate 4.000×10–5mol•L-1, and the maximum hemolytic activity reached 347.48HU•L-1 under the same condition.

Key words: Amphidinium klebsii, haemolytic toxin, nitrate, phosphate

CLC Number: 

  • Q178.53