长期高氨氮暴露对海水青鳉毒理效应的蛋白质组学研究

doi:10.11978/2016126

Abstract

Abstract:

Abstract: Most teleost fish excretes nitrogen waste as ammonia, but high environment ammonia is toxic to fish. In this study, we demonstrated that the death rate of marine medaka Oryzias melastigma exposed to 0.6 and 1.1 mmol·L^-1 NH₄Cl for four weeks were 21.25% and 40.00%, respectively, suggesting high environment ammonia is toxic to the fish. Besides, the higher concentration the ambient ammonia, the more toxic it is to the fish. We investigated the responses of chronic ammonia exposure in marine medaka using proteomics. Sixteen proteins were found to be remarkably altered in the fish exposed to 0.6 mmol·L^-1 NH₄Cl and seven proteins, in the fish exposed to 1.1 mmol·L^-1 NH₄Cl. The function of these proteins included inducing oxidative stress, neurotoxicity, disturbance in cell structure, muscle contraction, visual pathway, metabolic and immunological regulation. This is the first report of studying the toxicological effect of ammonia on marine medaka using proteomics. It provides important insights into toxicological effects of environmental contaminant using proteomics.

Key words: chronic ammonia exposure, proteomic, marine medaka, toxicological effect

Na GAO, Limei ZHU, Deliang YU, Li ZHANG. Proteomic responses of marine medaka (Oryzias melastigma) to chronic high environment ammonia exposure[J].Journal of Tropical Oceanography, 2017, 36(5): 40-48.

Figures/Tables 4

Fig. 1

Fig. 2

Tab. 1

List of proteins which were differentially expressed in O. melastigma induced by chronic ammonia exposure"

	蛋白点数	组别	蛋白名称		序列号	分子量/u	等电点	蛋白得分	序列覆盖率	匹配肽数	倍数变化
氧化应激	94	A	低分子量蛋白: 类线粒体的过氧化物还原酶5	青鳉	gi\|432899770	20491	8.68	114	0.18	2	4.26
	140	B	类蛋白酶体β亚基4	青鳉	gi\|432881057	28758	6.32	138	0.20	3	2.58
	178	B	胰蛋白酶原	青鳉	gi\|145966014	26708	5.16	255	0.12	1	1.63
细胞结构	285	A	类透明质酸蛋白聚糖连接蛋白1	青鳉	gi\|432874420	37805	8.18	63	0.03	1	-1.54
细胞结构	288	B	类透明质酸蛋白聚糖连接蛋白1	青鳉	gi\|432874420	37805	8.18	76	0.03	1	-1.71
肌肉收缩	92	A	类丝切蛋白-2	青鳉	gi\|432936587	18889	6.62	426	0.37	4	1.60
	343	A	类原肌球蛋白α1链亚型4	青鳉	gi\|432861692	31829	4.77	268	0.19	3	-1.70
	333	A	肌钙蛋白T, 类快骨骼肌型亚型2	青鳉	gi\|432860165	27699	9.41	96	0.10	2	-1.55
	403	B	类钙腔蛋白B	青鳉	gi\|432863493	29610	4.42	152	0.18	3	-1.92
视觉通路	176	A	中间丝蛋白ON3亚型2	青鳉	gi\|432864505	57350	5.06	69	0.02	1	1.63
	90	A	α-晶状体A链	青鳉	gi\|432958610	19919	5.95	184	0.44	6	1.70
	135	A	类β-晶状体B1链	青鳉	gi\|432878256	28885	8.36	70	0.23	4	4.88
	161	A	类β-晶状体A2链亚型1	青鳉	gi\|432964694	23855	6.09	377	0.51	5	2.07
代谢调节	17	A	14ku载脂蛋白部分片段	海水青鳉	gi\|328963182	11405	6.23	134	0.32	1	-1.53
	375	A	类线粒体ATP合成酶β亚基	青鳉	gi\|432849647	55238	5.10	562	0.24	6	2.15
	386	A	类α-烯醇酶	青鳉	gi\|432866138	45323	6.36	431	0.30	5	1.99
	207	B	类异戊烯基二磷酸Δ异构酶1	青鳉	gi\|432927313	32545	6.06	110	0.23	3	-3.27
免疫调节	153	A	类组织蛋白酶B	青鳉	gi\|432852559	37145	5.93	90	0.05	1	2.07
	42	A	类半乳凝素9	青鳉	gi\|432852360	23618	8.81	90	0.16	2	2.81
	559	B	类胎球蛋白B	大黄鱼	gi\|734621215	57367	5.99	63	0.01	1	2.75
神经毒性	18	A	类S100蛋白A	青鳉	gi\|432908621	11360	5.62	60	0.08	1	1.90
	391	A	突触囊泡膜蛋白VAT-1同系物	青鳉	gi\|432922308	46708	5.40	150	0.10	3	1.53
	847	B	类S100B亚型1	青鳉	gi\|432848584	11052	4.43	67	0.17	1	-2.70

Tab. 1

Fig. 3

References 45

[1]	陈文波, 李卫国, 张珍, 等, 2013. 斑马鱼3种胰蛋白酶原基因的克隆、序列分析及组织表达[J]. 中山大学学报(自然科学版), 52(1): 111-117.
	CHEN WENBO, LI WEIGUO, ZHANG ZHEN, et al, 2013. Molecular cloning, sequence analysis and tissue expression of three forms trypsinogens in zebrafish, Danio rerio[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 52(1): 111-117 (in Chinese with English abstract).
[2]	ACKERMAN P A, WICKS B J, IWAMA G K, et al, 2006. Low levels of environmental ammonia increase susceptibility to disease in Chinook salmon smolts[J]. Physiological and Biochemical Zoology, 79(4): 695-707.
[3]	ADAMI C, SORCI G, BLASI E, et al, 2001. S100B expression in and effects on microglia[J]. GLIA, 33(2): 131-142.
[4]	ADAMS J, 2004. The proteasome: A suitable antineoplastic target[J]. Nature Reviews Cancer, 4(5): 349-360.
[5]	AHSAN N, RENAUT J, KOMATSU S, 2009. Recent developments in the application of proteomics to the analysis of plant responses to heavy metals[J]. Proteomics, 9(10): 2602-2621.
[6]	ANDLEY U P, 2007. Crystallins in the eye: function and pathology[J]. Progress in Retinal and Eye Research, 26(1): 78-98.
[7]	AROCKIARAJ J, EASWVARAN S, VANARAJA P, et al, 2012. Immunological role of thiol-dependent peroxiredoxin gene in Macrobrachium rosenbergii[J]. Fish & Shellfish Immunology, 33(1): 121-129.
[8]	BOBKOV A A, MUHLRAD A, PAVLOV D A., et al, 2006. Cooperative effects of cofilin (ADF) on actin structure suggest allosteric mechanism of cofilin function[J]. Journal of Molecular Biology, 356(2): 325-334.
[9]	CHEN J C, LIU P C, LIN Y T, et al, 1988. Super intensive culture of red-tailed shrimp Penaeus penicillatus[J]. Journal of the World Aquaculture Society, 19(3): 127-131.
[10]	CHENG CHANGHONG, YANG FANGFANG, LING RENZHI, et al, 2015. Effects of ammonia exposure on apoptosis, oxidative stress and immune response in pufferfish (Takifugu obscurus)[J]. Aquatic Toxicology, 164: 61-71.
[11]	CHING BIYUN, CHEW S F, WONG W P, et al, 2009. Environmental ammonia exposure induces oxidative stress in gills and brain of Boleophthalmus boddarti (mudskipper)[J]. Aquatic Toxicology, 95(3): 203-212.
[12]	CHOI J W, LIU HAO, SONG H, et al, 2012. Plasma marker proteins associated with the progression of lung cancer in obese mice fed a high-fat diet[J]. Proteomics, 12(12): 1999-2013.
[13]	CONUS S, SIMON H U, 2008. Cathepsins: Key modulators of cell death and inflammatory responses[J]. Biochemical Pharmacology, 76(11): 1374-1382.
[14]	DOSDAT A, PERSON-LE RUYET J, COVÈS, et al, 2003. Effect of chronic exposure to ammonia on growth, food utilisation and metabolism of the European sea bass (Dicentrarchus labrax)[J]. Aquatic Living Resources, 16(6): 509-520.
[15]	EDDY F B, 2005. Ammonia in estuaries and effects on fish[J]. Journal of Fish Biology, 67(6): 1495-1513.
[16]	FEDORENKOVA A, VONK J A, LENDERS H J R, et al, 2010. Ecotoxicogenomics: Bridging the gap between genes and populations[J]. Environmental Science & Technology, 44(11): 4328-4333.
[17]	GIORDANO S, GLASGOW E, TESSER P, et al, 1989. A type II keratin is expressed in glial cells of the goldfish visual pathway[J]. Neuron, 2(5): 1507-1516.
[18]	HERNÁNDEZ-FONSECA K, CÁRDENAS-RODRÍGUEZ N, PEDRAZA-CHAVERRI J, et al, 2008. Calcium-dependent production of reactive oxygen species is involved in neuronal damage induced during glycolysis inhibition in cultured hippocampal neurons[J]. Journal of Neuroscience Research, 86(8): 1768-1780.
[19]	IVANČIČ I, DEGOBBIS D, 1984. An optimal manual procedure for ammonia analysis in natural waters by the indophenol blue method[J]. Water Research, 18(9): 1143-1147.
[20]	JI CHENGLONG, WU HUIFENG, WEI LEI, et al, 2013a. Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47)[J]. Aquatic Toxicology, 140-141: 449-457.
[21]	JI CHENGLONG, WU HUIFENG, WEI LEI, et al, 2013b. Responses of Mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics[J]. Fish & Shellfish Immunology, 35(2): 489-498.
[22]	KONDO H, MORINAGA K, MISAKI R, et al, 2005. Characterization of the pufferfish Takifugu rubripes apolipoprotein multigene family[J]. Gene, 346: 257-266.
[23]	KOSENKO E, KAMINSKI Y, LOPATA O, et al, 1999. Blocking NMDA receptors prevents the oxidative stress induced by acute ammonia intoxication[J]. Free Radical Biology and Medicine, 26(11-12): 1369-1374.
[24]	KOSHIKAWA N, HASEGAWA S, NAGASHIMA Y, et al, 1998. Expression of trypsin by epithelial cells of various tissues, leukocytes, and neurons in human and mouse[J]. The American Journal of Pathology, 153(3): 937-944.
[25]	KUMAR S, SHARMA J G, CHAKRABARTI R, 2007. Acute toxicity of ammonia to a freshwater teleost, Labeo bata larvae[J]. Toxicological & Environmental Chemistry, 89(2): 327-336.
[26]	LEMOS M F L, SOARES A M V M, CORREIA A C, et al, 2010. Proteins in ecotoxicology-how, why and why not?[J]. Proteomics, 10(4): 873-887.
[27]	LIEW H J, SINHA A K, NAWATA C M, et al, 2013. Differential responses in ammonia excretion, sodium fluxes and gill permeability explain different sensitivities to acute high environmental ammonia in three freshwater teleosts[J]. Aquatic Toxicology, 126: 63-76.
[28]	LIU XIOLI, Wu HUIFENG, JI CHENGLONG, et al, 2013. An integrated proteomic and metabolomic study on the chronic effects of mercury in Suaeda salsa under an environmentally relevant salinity[J]. PLoS One, 8(5): e64041.
[29]	OLEKSIAK M F, 2008. Changes in gene expression due to chronic exposure to environmental pollutants[J]. Aquatic Toxicology, 90(3): 161-171.
[30]	PERSON-LE RUYET J, CHARTOIS H, QUEMENER L, 1995. Comparative acute ammonia toxicity in marine fish and plasma ammonia response[J]. Aquaculture, 136(1-2): 181-194.
[31]	RANDALL D J, TSUI T K N, 2002. Ammonia toxicity in fish. Marine Pollution Bulletin, 45(1-2): 17-23.
[32]	ROSE-MARTEL M, SMILEY S, HINCKE M T, 2015. Novel identification of matrix proteins involved in calcitic biomineralization[J]. Journal of Proteomics, 116: 81-96.
[33]	SANTOS E M, BALL J S, WILLIAMS T D, et al, 2010. Identifying health impacts of exposure to copper using transcriptomics and metabolomics in a fish model[J]. Environmental Science & Technology, 44(2): 820-826.
[34]	SHINGLES A, MCKENZIE D J, TAYLOR E W, et al, 2001. Effects of sublethal ammonia exposure on swimming performance in rainbow trout (Oncorhynchus mykiss)[J]. Journal of Experimental Biology, 204(15): 2691-2698.
[35]	SINHA A K, ABDELGAWAD H, GIBLEN T, et al, 2014. Anti-oxidative defences are modulated differentially in three freshwater teleosts in response to ammonia-induced oxidative stress[J]. PLoS One, 9(4): e95319.
[36]	SINHA A K, DIRICX M, CHAN L P, et al, 2012. Expression pattern of potential biomarker genes related to growth, ion regulation and stress in response to ammonia exposure, food deprivation and exercise in common carp (Cyprinus carpio)[J]. Aquatic Toxicology, 122-123: 93-105.
[37]	SMITH R W, WOOD C M, CASH P, et al, 2005. Apolipoprotein AI could be a significant determinant of epithelial integrity in rainbow trout gill cell cultures: A study in functional proteomics[J]. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1749(1): 81-93.
[38]	SORCI G, AGNELETTI A L, DONATO R, 2000. Effects of S100A1 and S100B on microtubule stability. An in vitro study using triton-cytoskeletons from astrocyte and myoblast cell lines[J]. Neuroscience, 99(4): 773-783.
[39]	SPICER A P, JOO A, BOWLING JR R A, 2003. A hyaluronan binding link protein gene family whose members are physically linked adjacent to chrondroitin sulfate proteoglycan core protein genes: the missing links[J]. Journal of Biological Chemistry, 278(23): 21083-21091.
[40]	TIAN LI, WANG MINGHUA, LI XIAOMIN, et al, 2011. Proteomic modification in gills and brains of medaka fish (Oryzias melastigma) after exposure to a sodium channel activator neurotoxin, brevetoxin-1[J]. Aquatic Toxicology, 104(3-4): 211-217.
[41]	WADA J, KANWAR Y S, 1997a. Identification and characterization of galectin-9, a novel β-galactoside-binding mammalian lectin[J]. Journal of Biological Chemistry, 272(9): 6078-6086.
[42]	WADA J, OTA K, KUMAR A, et al, 1997b. Developmental regulation, expression, and apoptotic potential of galectin-9, a beta-galactoside binding lectin[J]. Journal of Clinical Investigation, 99(10): 2452-2461.
[43]	WOOD C M, NAWATA C M, 2011. A nose-to-nose comparison of the physiological and molecular responses of rainbow trout to high environmental ammonia in seawater versus freshwater[J]. Journal of Experimental Biology, 214(21): 3557-3569.
[44]	WU HUIFENG, LIU XIAOLI, ZHANG XINGYAN, et al, 2013. Proteomic and metabolomic responses of clam Ruditapes philippinarum to arsenic exposure under different salinities[J]. Aquatic Toxicology, 136-137: 91-100.
[45]	ZIMMERMANN H, 1982. Biochemistry of the isolated cholinergic vesicles[M]//KLEIN R L, LAGERCRANTZ H, ZIMMERMANN H. Neurotransmitter Vesicles. New York: Academic, 271: 304.

Proteomic responses of marine medaka (Oryzias melastigma) to chronic high environment ammonia exposure

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