Marine biology

Effects of different culture media on the growth and production of astaxanthin by Chlorella zofingiensis

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  • 1. South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, 510301; 2. Department of Botany, the Uni-versity of Hong Kong, Hong Kong, P. R. China
彭娟(1980—), 女, 湖北省松滋县人, 博士, 主要从事微藻生物技术研究。

Received date: 2008-01-07

  Revised date: 2008-04-08

  Online published: 2010-05-24

Supported by

中国科学院 “百人计划”课题资助

Abstract

The biomass and astaxanthin yield of Chlorella zofingiensis cultivated in three different media including CZ-M1, Kuhl and KM1 were investigated. Results showed that Kuhl medium was best for growth as reflected by its highest values of specific growth rate, maximum cell dry weight concentration and cell growth yield on glucose comparing with the other media. However, its astaxanthin production was the lowest. KM1 exhibited the highest astaxanthin production both on a per dry cell weight and a per volume basis. Higher biomass (up to 8.99g•L-1 cell dry weight concentration) and astaxanthin levels (up to 20.1mg•L-1 and 2.24mg•g-1) in glucose-stressed Chlorella zofingiensis cells growing under low light in modified KM1 medium were obtained.

Cite this article

PENG Juan,WANG Yan,XIANG Wen-zhou,CHEN Feng, . Effects of different culture media on the growth and production of astaxanthin by Chlorella zofingiensis[J]. Journal of Tropical Oceanography, 2010 , 29(3) : 61 -64 . DOI: 10.11978/j.issn.1009-5470.2010.03.061

References

[1] 刘子贻, 沈奇桂. 虾青素的生物活性及开发应用前景[J].中国海洋, 1997, 63(3): 46.
[2] 李浩明, 高  蓝. 虾青素的结构、功能与应用[J]. 精细化工, 2003, 20(1): 32-37.
[3] IP P F, CHEN F. Peroxynitrite and nitryl chloride enhance astaxanthin production by the green microalga Chlorella zofingiensis in heterotrophic culture[J]. Process Biochemistry, 2005, 40: 3595-3599.
[4] 陈涛, 向文洲, 何慧, 等. 不同碳源对小球藻Chlorella zofingiensis异养产虾青素的影响[J]. 微生物学通报, 2007, 34(5): 856-858.
[5] CHEN F, ZHANG Y M, GUO S Y. Growth and phycocyanin formation of Spirulina platenisis in photoheterotrophic cul-ture[J]. Biotechnology Letters, 1996, 18 (5): 603-608.
[6] CHEN F, JOHNS M R. Relationship between substrate inhi-bition and maintenance energy of Chlamydomonas reinhard-tii in heteterotrophic culture
[J]. Journal of Applied Phycol-ogy, 1996, 8(1): 15-19.
[7] MILLER G L. Use of dinitrosalicylic acid reagent for deter-mination of reducing sugar [J]. Anal Chem, 1959, 31: 426-427.
[8] YUAN J P, CHEN F. Purification of trans-astaxanthin from a high-yielding astaxanthin ester-producing strain of the mi-croalga Haematococcus pluvialis [J]. Food Chemistry, 2000, 68: 443-438. 

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