Journal of Tropical Oceanography >
Study on the secondary metabolites of fungus Penicillium sp. SCSIO 40438 from the South China Sea
Copy editor: YAO Yantao
Received date: 2022-04-14
Revised date: 2022-05-08
Online published: 2022-05-10
Supported by
Guangdong MEPP Funds(GDNRC[2021]48)
K.C.Wong Education Foundation(GJTD-2020-12)
Key Area Research and Development Program of Guangdong Province(2020B1111030005)
Special Support Program for Training High Level Talents in Guangdong(2019TQ05H299)
This experiment aims to study the secondary metabolites of fungus Penicillium sp. SCSIO 40438 from the South China Sea. The solid fermentation products of the strain were isolated and purified by a variety of separation methods, including silica gel column, Sephadex LH-20 gel column, and preparative high performance liquid chromatography. The structures of the isolated compounds were identified by nuclear magnetic resonance, high resolution electrospray ionization mass spectroscopy and comparison with the previously reported data. Nine compounds were obtained and identified as 1-(2-Methylbut-3-en-2-yl)-1H-indole-3-carbaldehyde (1), 1-methyl-2(1H)-quinazolinone (2), fructigenine A (3), fructigenine B (4), 2-[(s)-hydroxy(phenyl)methyl]-3-methylquinazolin-4(3H)-one (5), 3-Methylviridicatin (6), 3-O-methylviridicatol (7), viridicatol (8), (+)-cyclopenol (9), and compounds 1 and 2 are two new natural products. Compound 8showed moderate antibacterial activities against Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus with minimal inhibition concentration (MIC) values of 8.0 μg·mL-1.
Keywords fungi; Penicillium sp.; secondary metabolites; antibacterial activity
ZHU Yiguang , MOU Pengyun , ZHANG Qingbo , ZHANG Changsheng . Study on the secondary metabolites of fungus Penicillium sp. SCSIO 40438 from the South China Sea[J]. Journal of Tropical Oceanography, 2023 , 42(1) : 161 -167 . DOI: 10.11978/2022079
表1 化合物1—9的抑菌活性测试结果Tab. 1 The antibacterial activities of compounds 1~9 |
化合物 | M. Luteus SCSIO ML01 | S. Aureus ATCC 29213 | MRSA ATCC 43300 | P. aeruginosa ATCC 27853 | A. baumannii ATCC 19606 | E.coli ATCC 25922 |
---|---|---|---|---|---|---|
1 | 32.0 | >64.0 | 32.0 | >64.0 | >64.0 | >64.0 |
2 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
3 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
4 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
5 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
6 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
7 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
8 | >64.0 | 8.0 | 8.0 | >64.0 | >64.0 | >64.0 |
9 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 | >64.0 |
对照 | 0.5a | 0.5a | 0.5a | 1.0b | 1.0b | 1.0b |
注:a为万古霉素, b为环丙沙星 |
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