The false mussel Mytilopsis sallei is an important invasive fouling organism in south of China, which is often attaching to aquaculture facilities and causes fishery losses. In this study, the ecological single factor gradient test was used to test larval settlement and metamorphosis of M. sallei under different salinities, water temperatures, light intensities and dissolved oxygen concentrations. The results showed that the suitable temperature for larval settlement and metamorphosis of M. sallei was 25~35℃. Low temperatures (10℃ and 15℃) inhibited larval settlement and metamorphosis, while high temperature (40℃) caused the death of all larvae. The suitable salinity was 15‰~30‰, with the optimal salinity of 15‰. The suitable light intensity was 0~5000 lux, but when light intensity reached more than 10000 lux, larval settlement and metamorphosis were significantly inhibited. The suitable dissolved oxygen concentration was 40%~100% of saturated oxygen concentration (3.2~8.0 mg·L^-1), but when the dissolved oxygen concentration is lower than 20% (1.6 mg·L^-1), larval settlement and metamorphosis were inhibited. M. sallei larvae was suitable to settle and metamorphose under high temperature (25~35℃), wide salinity (15‰~30‰) and low light intensity (0~5000 lux), with strong resistance capacity to low dissolved oxygen concentration. This study revealed the suitable environmental conditions on larval settlement and metamorphosis ofM. sallei, which is important for predicting its possible distribution in the coastal areas of China and preventing its invasion to other areas of China and causing aquaculture industry losses.