In order to reveal the photosynthetic response of intertidal macroalgae to temperature rise and their photosynthetic efficiency under different light qualities, and to provide a theoretical basis for the construction of island seaweed farms and the cultivation of seaweed. We usd chlorophyll fluorescence and dissolved oxygen sensors to examine the photosynthetic responses of four macroalgae (Ulva pertusa, Grateloupia livida, Sargassum thunbergii and Sargassum fusiforme) from the intertidal zone of Lvhua Island to temperature (15, 20, 25℃, respectively) and light quality (white, red, green and blue). The results showed that temperature rise reduced the maximum quantum yield (Fv/Fm) of Photosystem II (PSII) in these seaweeds, but significantly enhanced the actual quantum yield (Y(Ⅱ)), ratio of photosynthetic repair to damage rate (r/k) and photochemical quenching (qP) under strong light (900μmol photons m-2·s-1). Temperature rise significantly increased the non-photochemical quenching (NPQ) of S. fusiforme, but the contrary was observed in G. livida. Compared to other seaweeds, S. thunbergii exhibits the highest maximum relative electron transfer rate (rETRmax), half-saturated light intensity (Ek), qP and r/k. Under different light qualities, these seaweeds show no significant differences in maximum photosynthetic rate (Pmax) under white, red and green light, but Pmax was significantly reduced under blue light, with the greatest decrease observed in U. pertusa. As a result, the photosynthetic responses of different species to temperature rise and light quality varied considerably, the short-term warming helps the four intertidal macroalgae to resist strong light and improve their photosynthetic activity under strong light at low tide. And S. thunbergii was more adapted to strong light and temperature rise than the others. Additionally, the higher photosynthetic efficiency under red and green light reflects their adaptation to the shallow water in the intertidal zone.