To evaluate the stock enhancement effectiveness of Acanthopagrus latus, this study employed highly polymorphic microsatellite markers developed by our research group using the species' genome and established a parentage assignment technology. Based on this, we assessed the stock enhancement effectiveness of A.latus in Xiamen Bay from 2023 to 2024. The specific findings are as follows: (1) Polymorphic Primer Screening: From 110 randomly selected microsatellite loci in the A.latus genome, we designed primers and preliminarily screened 9 loci with high polymorphism using a wild A.latus population. (2) Establishment of Parentage Assignment Technology: Using known pedigree families of A.latus, including 20 parental fish and 72 offspring, we performed parentage analysis with the 9 selected microsatellite loci. The results detected 145 alleles across the loci, with an average allele number (N_a) of 16.11, an average observed heterozygosity (H_o) of 0.788, an average expected heterozygosity (H_e) of 0.810, and an average polymorphic information content (PIC) of 0.785, indicating high polymorphism. The analysis using Cervus3.0.7 software showed that under a 95% confidence level, the parentage assignment success rate was 98.61% when the sex of both parents was known, and 97.22% when the sex was unknown. (3) Stock Enhancement Evaluation: The parentage assignment technology was applied to 114 released parental fish and 264 recaptured individuals. Among the 264 recaptured A.latus, 8 were identified as offspring of the released parents, indicating a resource contribution rate of 3.03% to the wild A.latus population.The results provide reliable technical methods for future genetic resource identification and stock enhancement evaluation of A.latus.