The secondary metabolites produced by Aspergillus fumigatus 9-1, isolated from the red alga Gelidium, were systematically separated and purified using a combination of TLC, silica gel column chromatography, HPLC, and gel column chromatography, resulting in the isolation of 16 compounds. Spectral analysis, including NMR spectroscopy and comparison with literature data, led to the structural elucidation of these compounds as: 12R,13S-dihydroxyfumitremorgin C (1), helvolic acid (2), questin (3), pyripyropene A (4), verruculogen TR-2 (5), cyclotryprostatin E (6), cyclotryprostatin B (7), asperfumigatin (8), spirocyclic diketopiperazine alkaloid (9), fumigaclavine C (10), monomethylsulochrin (11), thymidine (12), fumigaclavine A (13), chaetominine (14), prenylcyclotryprostatin A (15), fumiquinazoline J (16). Compound 15 was first discovered from the genus Aspergillus. Compound 2 exhibited potent antibacterial activity against Listeria monocytogenes, with a minimum inhibitory concentration (MIC) of 4.03 μg·mL ^-1. Compound 3, 4 and11 demonstrated moderate antibacterial activity against L. monocytogenes, with MIC values of 64.54 μg·mL ^-1, 66.25 μg·mL ^-1, and 78.63 μg·mL ^-1, respectively. Compound 1 displayed strong DPPH radical scavenging activity. Furthermore, compounds 1, 10, 11, and 13 exhibited potent ABTS radical scavenging activity.