Journal of Tropical Oceanography >
Degradation effects of Bacillus licheniformis strain De on shrimp (Litopenaeus vannamei) dejecta
Received date: 2008-05-30
Revised date: 2008-11-12
Online published: 2010-07-29
Supported by
国家“十一五”支撑项目(2006BAD03B0106, 2006BAD09A07); 中央级科研院所基本科研专项(2007ZD01); 广东省科技计划
(2007A020400001); 广东省重大科技兴渔项目(A200899A06); 公益性农业行业专项(nyhyzx07-042\200803012); 现代农业(虾)
产业技术体系建设专项 (nycytx46); 广东省鱼病防治专项(2130108)
The degradation activity of Bacillus licheniformis De strain on shrimp dejecta was analyzed by an independent analytical method. The interference effects are avoidance, including biologic or environmental factors in ecosystem of aquaculture ponds. Dejecta of Litopenaeus vannamei was collected, dehydrofreezed, and dissolved in disinfected sea water after comminuted as powder. The degradation effects of NH3—N, NO2-—N, NO3-—N, PO4-—P and COD in the dejecta solution were studied at different temperature (16, 21, 26 and 31℃), dejecta (5, 10 and 20mg·L-1) and Bacillus concentration (1, 2.5, 5 and 10mg·L-1). The results show that concentrations of both COD and NO3-—N decreased; their average degradation rates respectively exceeded 60% and 50%, but the concentrations of NH3—N、NO2-—N and PO4-—P all continually increased. There were significant effects (p<0.05) of temperature and of all parameters, with optimum conditions respectively at 26—31ºC and Bacillus concentration of 5mg·L-1. The differences of each two groups were insignificant (p>0.05) as the dejecta concentration exceeded 10mg·L-1.
Key words: Bacillus licheniformis; Litopenaeus vannamei; dejecta; degradation effects
CAO Yu-cheng,LI Zhuo-jia,LIN Xiao-tao,YANG Ying-ying . Degradation effects of Bacillus licheniformis strain De on shrimp (Litopenaeus vannamei) dejecta[J]. Journal of Tropical Oceanography, 2010 , 29(4) : 125 -131 . DOI: 10.11978/j.issn.1009-5470.2010.04.125
[1] 李松青, 林小涛, 李卓佳, 等. 摄食对凡纳滨对虾耗氧率和氮、磷排泄率的影响[J]. 热带海洋学报, 2006, 25(2): 44-48.
[2] 郝桂玉, 徐亚同, 黄民生, 等. 芽孢杆菌脱氮作用的研究[J]. 环境科学与技术, 2004, 27(1): 20-21.
[3] 刘波, 刘文斌. 芽孢杆菌对水产养殖环境的净化作用[J].渔业现代化, 2004, 22: 7-8.
[4] 熊伟, 梁运祥, 戴经元, 等. 枯草芽孢杆菌对斑节对虾饲养池水净化作用的初步研究[J]. 华中农业大学学报, 2003, 22(3): 247-250.
[5] 刘颖, 丁桂珍, 胡传红, 等. 枯草芽孢杆菌对养殖水体水质影响研究[J]. 淡水渔业, 2004, 34(5): 12-14.
[6] LIN HEIZHAO, GUO ZHIXUN, YANG YINGYING, et al. Effect of dietary probiotics on digestibility coefficients of nutrients of white shrimp Litopenaeus vannamei Boone[J]. Aquaculture Research, 2004, 35: 1441-1447.
[7] 曹煜成, 李卓佳, 冯娟, 等. 地衣芽孢杆菌胞外产物消化活性的研究 [J]. 热带海洋学报, 2006, 24(6): 6-12.
[8] 李卓佳, 张庆, 陈康德, 等. 应用微生物健康养殖斑节对虾的研究[J]. 中山大学学报, 2000, 39(增刊): 229-232.
[9] 国家质量技术监督局. 海洋监测规范——海水分析(GB17378.4—1998)[S]. 北京: 中国标准出版社, 1998: 142-162.
[10] 布坎南, 吉本斯. 伯杰细菌鉴定手册[M]. 北京: 科学出版社, 1984: 730-737.
[11] GATESOUPE F J. The use of probiotics in aquaculture [J]. Aquaculture, 1999, 160: 177-203.
[12] WELLINGTA C A N, MEIRE L L M. Production and properties of an extracellular protease from thermophilic Bacillus sp. [J]. Brazilian Journal of Microbiology, 2004, 35: 91-96.
[13] ÇALIK P, BILIR E, ÇALIK G, et al. Influence of pH conditions on metabolic regulations in serine alkaline protease production by Bacillus licheniformis
[J]. Enzyme and Microbial Technology, 2002, 31: 685-697.
[14] MAITIN V, KAVITHA R, UMESH-KUMAR S. Properties of an extracellular amylase purified from a Bacillus species[J]. World Journal of Microbiology and Biotechnology, 2001, 17: 823-826.
[15] 曹煜成, 李卓佳, 吴灶和, 等. 地衣芽孢杆菌胞外蛋白酶的纯化及特性分析[J]. 水生生物学报, 2006, 30(3): 262-268.
[16] 李卓佳, 曹煜成, 陈永青, 等. 地衣芽孢杆菌De株的胞外产物对凡纳滨对虾脂肪酶活性影响的体外试验[J]. 高技术通讯, 2006, 16(2): 191-195.
[17] 周德庆. 微生物学教程[M]. 北京: 高等教育出版社, 1999: 140-141.
[18] 沈南南, 李纯厚, 贾晓平, 等. 3种微生物制剂调控工厂化对虾养殖水质的研究[J]. 南方水产, 2007, 3(3): 20-25.
[19] 刘朝阳, 孙晓庆. 生物控制法在水产养殖水质净化中的综合应用[J]. 南方水产, 2007, 3(1): 69-74.
/
〈 |
|
〉 |