Journal of Tropical Oceanography >
Effects of temperature and pH on the digestive enzymes activities of juvenile striped beakperch Oplegnathus fasciatus
Received date: 2008-10-15
Revised date: 2009-02-23
Online published: 2011-10-10
Supported by
中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放基金(2007A014)
The effects of temperature and pH on digestive enzymes activities (stomach, intestine and liver) were investigated in juvenile striped beakperch Oplegnathus fasciatus. The results indicated that the optimum temperature for protease in intes-tine and liver was 25℃, and that in stomach was 28℃. The optimum temperature for amylase in stomach and liver was 22℃, and that in intestine was 19℃. The optimum temperature for lipase in three digestive organs was all 16℃. During 16℃ to 28℃, the protease and lipase both had relatively high activities, however, the amylase had a lower activity. The optimum pH values for protease, amylase and lipase were 3.2, 7.6 and 4.2 in stomach, and 8.6, 6.6 and 6.6 in intestine, respectively. The optimum pH in liver was 7.6 for protease, and 6.6 for amylase and lipase.
LUO Qi,OU You-jun,AI Li,LI Jia-er . Effects of temperature and pH on the digestive enzymes activities of juvenile striped beakperch Oplegnathus fasciatus[J]. Journal of Tropical Oceanography, 2010 , 29(5) : 154 -158 . DOI: 10.11978/j.issn.1009-5470.2010.05.154
[1] 牛化欣, 常杰, 马甡. 条石鲷的生物学及养殖生态学研究进展[J]. 水产科技情报, 2008, 1: 22-23.
[2] 吴勇, 区又君, 李希国. 消化酶活力在千年笛鲷幼鱼不同消化器官中的比较研究[J]. 南方水产, 2006, 2 (2): 61-63.
[3] FLOWERDEW M W, GROVE D J. Some observations of the effects of body weight, temperature, meal size and quality on gastric emptying time in turbot, Scophthalmus maximus (L.) using radiography [J]. J Fish Biol, 1979, 14: 229-238.
[4] CHESLEY L C. The concentration of proteases, amylases, and lipase in certain marine fishes[J]. Biol Bull, 1934, 66(2): 133-144.
[5] 陈品健, 王重刚, 黄崇能, 等. 真鲷仔、稚、幼鱼期消化酶活性的变化[J]. 台湾海峡, 1997, 16 (3): 245-248.
[6] 区又君, 刘泽伟. 千年笛鲷幼鱼的饥饿和补偿生长[J]. 水产学报, 2007, 31(3): 323-328.
[7] 付新华, 孙谧, 孙世春. 大菱鲆消化酶的活力[J]. 中国水产科学, 2005, 12 (1): 26-32.
[8] 王富荣. 生物工程分析与检验[M]. 北京: 中国轻工业出版社, 2005: 204-207.
[9] 胡思玉, 白建梅. 昆明裂腹鱼淀粉酶活性研究[J]. 水产科学, 2007, 26(4): 234-236.
[10] 陈品健, 王重刚, 郑森林. pH影响真鲷仔、幼鱼蛋白酶活性的研究[J]. 海洋学报, 1997, 19 (3): 97-101.
[11] 黄小燕, 吴天星, 李军, 等. 鮸鱼消化酶活性的研究[J]. 水利渔业, 2006, 24(4): 86-88.
[12] 叶继丹, 卢彤岩, 刘洪柏, 等. 六种鲟鱼消化酶活力的比较研究[J]. 水生生物学报, 2003, 27 (6): 590-595.
[13] 李希国, 李加儿, 区又君. 温度对黄鳍鲷主要消化酶活性的影响[J]. 南方水产, 2006, 2(1): 43-48.
[14] 田宏杰, 庄平, 章龙珍, 等. 水温对施氏鲟幼鱼消化酶活力的影响[J]. 中国水产科学, 2007, 14(1): 126-131.
[15] UGOLEV A M, EGOROVA V V, KUZMINA V V, et al. Comparative molecular characterization of membrane digestion in fish and mammals[J ]. Comp Biochem Physiol, 1983, 76B: 627-635.
[16] MUNILLA-MORAN R, SABORIDO-REY F. Digestive enzymes in marine species. Ⅱ. Amylase activities in gut from seabream (Sparus aurata), turbot (Scophthalmus maximus) and redfish (Sebastes mentella) [J]. Comp Biochem Physiol, 1996, 113B (4): 827–834.
[17] 沈文英, 祝尧荣, 钱科亮. 温度和pH对澳洲宝石鱼消化酶活性的影响[J]. 大连水产学院学报, 2006, 21(2): 189-192.
[18] 李希国, 李加儿, 区又君. pH对黄鳍鲷主要消化酶活性的影响[J]. 南方水产, 2005, 1(6): 18-22.
[19] 尾崎久雄著, 吴尚忠译. 鱼类消化生理学[M]. 上海: 上海科技出版社, 1983: 104-105.
/
〈 | 〉 |