Journal of Tropical Oceanography >
Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata
Received date: 2009-01-22
Revised date: 2009-11-03
Online published: 2010-12-15
Supported by
国家自然基金面上项目(30770283); “十一五”国家科技支撑计划重点项目(2007BAD29B03); 海南省教育厅高等学校科学研
究资助项目(Hjkj2008-22); 广东省科技兴海(渔)项目(B200701A06)
Serum levels of gonadotropin (GtH), testosterone (T), 11-ketotestosterone (11-KT), and estradiol-17β (E2) were assessed by radioimmunoassay during induction of sexual maturation of male marbled eel (Anguilla marmorata) by Human Chorionic Gonadotropin (HCG) treatment. Morphological alterations of the liver and GtH cell of Anguilla marmorata were investigated by means of light and electron microscopy. The testis Gonadosome Index (GSI) was 0.17±0.06% in the control group and their serum GtH and 11-KT levels were low, the T level was lower than 0.1 ng (the range of kit was 0.1―20ng•mL?1); Serum GtH, T, 11-KT levels increased rapidly in the operated group after the third injection of HCG, when the GSI of testis was 7.0±0.03%. After the spermiation, the GtH, T and 11-KT levels decreased a little, but still higher than the levels of the control group. Serum E2 levels decreased gradually as HCG treatment progressed, from 2.36 ng/ml of the control group to 0.83 ng/ml of the spermiation eels. In the hepat of the control group, abundant glycongen and little lipid droplets were obversived, the nuclear was in the middle of the cell. In contrast, in the hepat of the spermiation eel, the lipid droplets accu-mulated, which lead to the closing of nuclear to the cell membrane.
QI Xin,HUANG Hai,ZHOU Wen-yi,YIN Shao-wu,ZHANG Yong,LIU Xiao-chun, . Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata[J]. Journal of Tropical Oceanography, 2010 , 29(6) : 130 -136 . DOI: 10.11978/j.issn.1009-5470.2010.06.130
[1] YAMAMOTO K, YAMAUCHI K. Sexual maturation of Japanese eel and production of eel larvae in the aquarium [J]. Nature 1974, 251: 220–222.
[2] 王义强, 赵长春, 施正峰, 等. 河鳗人工繁殖的初步研究 [J]水产学报, 1980, 4(2): 147-156.
[3] 赵长春, 谭玉钧, 施正峰, 等. 产后鳗鲡再生的可能性 [J]海洋与湖沼, 1980, (3): 241-245.
[4] OKAMURA A, YAMADA Y, HORITA T. et al. Rearing eel leptocephali (
[5] PEDERSEN B H. Fertilisation of eggs, rate of embryonic development and hatching following induced maturation of the European eel
[6] 周雯伊, 黄海, 尹绍武, 等. 人工诱导花鳗鲡的精巢发育成熟及其精子的生物学特性[J]. 水产学报, 2009, 33: 53-57.
[7] DUFOUR S, NADINE LE-BELLE, FONTAINE Y A. Effects of steroid hormones on pituitary immunoreactive gonadotropin in European freshwater eel,
[8] DUFOUR S, NADINE LE-BELLE, BALOCHE S. Positive feedback control by the gonads on gonadotropin (GtH) and gonadoliberin (GnRH) levels in experimentally matured female silver eels,
[9] 林浩然, 张梅丽, 张素敏, 等. 鳗鲡繁殖生物学研究IV. 人工催熟过程中下海鳗鲡的GtH分泌活动、性腺发育状况和脑垂体GtH细胞的超显微结构[J]. 水生生物学报, 1987, 11(4): 320-330.
[10] 林浩然, 张梅丽. 鳗鲡繁殖生物学研究Ⅴ: 性类固醇激素诱导雌鳗促性腺激素(GtH)分泌和卵巢发育的作用[J]. 水生生物学报, 1994(3): 272-279.
[11] 汪小东, 林浩然. 鳗鲡繁殖生物学研究Ⅵ: 鳗鲡17α, 20β-双羟孕酮的生成和作用. [J]动物学报, 1999, 45(3): 317-322
[12] 张利红, 张为民, 林浩然. 雄烯二酮和甲基睾酮诱导雄性日本鳗鲡性腺发育的作用[J]. 动物学研究, 2001, 22(2): 89-9.
[13] 汪小东, 谢刚, 林浩然. 鲤脑垂体匀浆液和人绒毛膜促性腺激素混合注射对鳗鲡脑区促性腺激素释放激素和血清促性腺激素及性类固醇激素浓度的影响[J]. 水产学报, 2000, 24(2): 123-129.
[14] 林浩然. 鱼类生理学[M]. 广州: 广东省高教出版社, 1999, 181-196.
[15] WOODS L C, SULLIVAN C V. Reproduction of striped bass, Morone saxatilis (Walbaum), broodstock: monitoring maturation and hormonal induction of spawning [J]. Aquaculture and Fisheries Management. 1993, 21(2): 211-222.
[16] MYLONAS C C, ZOHAR Y, WOODS L C, et al. Hormone profiles of captive striped bass Morone saxatilisduring spermiation and long-term enhancement of milt production [J]. J World Aquacult Soc, 1998, 29(4): 379-392.
[17] JACKSON L F, SULLIVAN C V. Reproduction of white perch: the annual gametogenic cycle [J]. Transactions of the American Fisheries Society, 1995, 124(4): 563-577.
[18] PART F S, ZANUY M, CARILLO A, et al. Seasonal changes in plasma levels of gonadal steroids of sea bass, Dicentrarchus labrax L [J]. Gen Comp Endocrinol, 1990, 78(3): 361-363.
[19] BERLINSKY D L, JACKSON L F, SMITH T I J, et al. The annual reproductive cycle of the white bass Morone chrysops [J]. J World Aquacult Soc, 1995, 26(3): 252-260.
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