Marine biology

Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata

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  • 1. College of Ocean, Hainan University, Key Laboratory of Tropical Biology Resources, Ministry of Education, Haikou 570228, China; 2. Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China
齐鑫(1983—), 男, 辽宁省沈阳市人, 硕士研究生, 主要从事鱼类生理学研究。E-mail: forever_qixin@yahoo.com.cn

Received date: 2009-01-22

  Revised date: 2009-11-03

  Online published: 2010-12-15

Supported by

国家自然基金面上项目(30770283); “十一五”国家科技支撑计划重点项目(2007BAD29B03); 海南省教育厅高等学校科学研
究资助项目(Hjkj2008-22); 广东省科技兴海(渔)项目(B200701A06)

Abstract

Serum levels of gonadotropin (GtH), testosterone (T), 11-ketotestosterone (11-KT), and estradiol-17β (E2) were assessed by radioimmunoassay during induction of sexual maturation of male marbled eel (Anguilla marmorata) by Human Chorionic Gonadotropin (HCG) treatment. Morphological alterations of the liver and GtH cell of Anguilla marmorata were investigated by means of light and electron microscopy. The testis Gonadosome Index (GSI) was 0.17±0.06% in the control group and their serum GtH and 11-KT levels were low, the T level was lower than 0.1 ng (the range of kit was 0.1―20ng•mL?1); Serum GtH, T, 11-KT levels increased rapidly in the operated group after the third injection of HCG, when the GSI of testis was 7.0±0.03%. After the spermiation, the GtH, T and 11-KT levels decreased a little, but still higher than the levels of the control group. Serum E2 levels decreased gradually as HCG treatment progressed, from 2.36 ng/ml of the control group to 0.83 ng/ml of the spermiation eels. In the hepat of the control group, abundant glycongen and little lipid droplets were obversived, the nuclear was in the middle of the cell. In contrast, in the hepat of the spermiation eel, the lipid droplets accu-mulated, which lead to the closing of nuclear to the cell membrane.

Cite this article

QI Xin,HUANG Hai,ZHOU Wen-yi,YIN Shao-wu,ZHANG Yong,LIU Xiao-chun, . Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata[J]. Journal of Tropical Oceanography, 2010 , 29(6) : 130 -136 . DOI: 10.11978/j.issn.1009-5470.2010.06.130

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