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Journal of Tropical Oceanography >

2011 , Vol. 30 >Issue 1: 86 - 90

DOI: https://doi.org/10.11978/j.issn.1009-5470.2011.01.086

Marine biology

Isolation, culture, and identification of a picophytoplankton

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  • 1. South China Sea institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate University of CAS, Beijing 100039, China
左云龙(1982—), 男, 山东省郯城县人, 硕士研究生, 从事微藻生物技术研究。

Received date: 2009-01-16

  Revised date: 2010-05-14

  Online published: 2011-03-16

Supported by

广东省科技计划项目(2009B080701093); 广东省海洋与渔业局科技专项项目(A2008009-08)

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Abstract

In this study, strain SCSIO-8, an isolated picophytoplankton was obtained from the continental shelf of the northern South China Sea. Studies were carried out on its growth, morphological structure, physiological and biochemical characteris-tics and taxonomic identification. The results indicate that SCSIO-8 was a free unicellular alga without flagellate, (2.08±0.28)×(1.78±0.09)µm in size, and no pyrenoid, 1 chloroplast, 1 mitochondria, 1 nucleus, and many starch granules were observed in its cell. It was revealed that SCSIO-8 reproduced through autosporation. Results also indicate that SCSIO-8 was able to grow in a wide range of salinity from 0 to 38‰ at least, and adapted rapidly to the mutual switch between seawater and freshwater. HPLC chromatogram analysis showed that the main photosynthetic pigments of SCSIO-8 were chlorophyll a, chlorophyll b, and several carotenoids including cis- and trans-Violaxanthin, Lutein, β-carotene, etc.18S rDNA region of SCSIO-8 genome DNA was sequenced and blasted in GenBank. The picophytoplankton strain could be identified as Pi-cochlorum sp. SCSIO-8 through integrated analysis of its morphological, physiological, biochemical, and molecular charac-teristics.

Key words：  picophytoplankton; Picochlorum; taxonomic identification; 18S rDNA

Cite this article

ZUO Yun-long,XIANG Wen-zhou,LIU Ji-hua,SU Jiao-jiao,HE Hui,ZHANG . Isolation, culture, and identification of a picophytoplankton[J]. Journal of Tropical Oceanography, 2011 , 30(1) : 86 -90 . DOI: 10.11978/j.issn.1009-5470.2011.01.086

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