Journal of Tropical Oceanography >
Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae
Received date: 2009-05-12
Revised date: 2010-06-22
Online published: 2011-03-16
Supported by
国家自然科学基金项目(30070595); 国家“863”计划项目(2006AA10A414)
Studies on karyotype, Ag-NORs, and C-banding of Lutjanus sebae were performed. The chromosomes were re-ceived from the head kidney by using a method of injecting with PHA and colchicines, air drying, and Giemsa staining. The results were as follows. 1) The karyotypic formula of L.sebae was 2n=48t, NF=48. Meanwhile, a pair of chromosomes, with secondary constriction near the centromere, was found on chromosome 1. 2) The Ag-NORs polymorphisms were individually specific in this fish. The silver staining pots were 1-4, and the number of Ag-NORs was mainly 2 (79%). A pair of nucleolar organizer regions was observed on the secondary constriction of chromosome 1, and no Ag-NORs combination was found. 3) Centromeres of most chromosomes were C-bandings positive, and heterochromatin was detected at the satellite zone of chro-mosome 1. Thus, we discussed the evolution of karyotype, the developing mechanism of Ag-NORs, and C-bandings for fish. The phylogenetic condition of L. sebae was evaluated as well.
Key words： Lutjanus seba; karyotype; Ag-NORs; C-banding
GUO Ming-lan,YOU Xin-xin,SU Yong-quan,DING Shao-xiong,WANG Jun . Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae[J]. Journal of Tropical Oceanography, 2011 , 30(1) : 91 -95 . DOI: 10.11978/j.issn.1009-5470.2011.01.091
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