Journal of Tropical Oceanography >
Optimization of medium components and culture conditions of algicidal actinomy-cetes BS01
Received date: 2010-07-26
Revised date: 2010-09-15
Online published: 2011-05-04
Supported by
国家高技术研究发展计划项目(2008AA09Z408); 国家自然科学基金项目(40930847、40876061); 滨海湿地生态系统教育部重点实验室开放基金项目(CWe0902); 福建省自然科学基金项目(2010J01223); 福建省教育厅项目(JA10232)
A strain of actinomycetes BS01 capable of lysing the toxic dinoflagellate Alexandrium tamarense was isolated from mangrove area of Zhangjiangkou in Fujian province. The medium components and cultural conditions for algicidal activity of actinomycete strain BS01 were optimized by single-factor and uniform tests in shake-flask. The initial pH, fer-mentation temperature, fermentation time, and inoculum were optimized through single-factor tests. Results showed that BS01 exhibited the best algicidal activity by using soluble starch as carbon source, sodium nitrate as nitrogen source, initial pH at 7.7, temperature at 27.2℃, and shaking at 150r•min-1 in 100mL of 250mL conical flask for 48h. The optimal fer-mentation parameters of BS01 were: soluble starch 20g•L-1, sodium nitrate 0.5g•L-1, pH value 7.7, and temperature 27.2℃ by using uniform design experiments.
FU Li-jun,AN Xin-li,LI Dong,XU Li-xia,TIAN Yun,ZHENG Tian-ling, . Optimization of medium components and culture conditions of algicidal actinomy-cetes BS01[J]. Journal of Tropical Oceanography, 2011 , 30(2) : 109 -114 . DOI: 10.11978/j.issn.1009-5470.2011.02.109
[1] KRIENITZ L, BALLOT A, KOTUT K, et al. Contribution of hot spring cyanobacteria to the mysterious death of Lesser Flamingos at Lake Bogoria Kenya[J]. FEMS Microbiology Ecology, 2003, 43(2): 144-148.
[2] IMAMURA N, MOTOIKEL,SHIMADA N, et al. An efficient screening approach for anti-microcystis compounds based on knowledge of aquatic Microbial ecosystem[J]. J Antibiot, 2001, 54(6): 582-587.
[3] 赵以军, 刘永定. 有害藻类及其微生物防治的基础——藻菌关系的研究动态[J]. 水生生物学报, 1996, 20(2): 173-181.
[4] 王新, 周艳艳, 郑天凌. 海洋细菌生态学的若干重要领域及其研究新进展[J]. 微生物学报, 2010, 50(3): 291-297.
[5] 王新, 李志江, 郑天凌. 海洋浮游细菌在东海赤潮高发区的分布与活性[J]. 环境科学, 2010, 31(2): 287-295.
[6] WANG XIN, LI ZHIJIANG, SU JIANQIANG, et al. Lysis of a red-tide causing alga, Alexandrium tamarense, caused by bacteria from its phycosphere[J]. Biological Control, 2010, 52(2): 123-130.
[7] 郑天凌, 田蕴, 苏建强, 等. 海洋微生物研究的回顾与展望[J]. 厦门大学学报:自然科学版, 2006, 45(增刊2): 151-157.
[8] 吴刚, 席宇, 赵以军. 溶藻细菌研究的最新进展[J]. 环境科学研究, 2002, 15(5): 43-46.
[9] RASHIDAN K K, BIRD D F. Role of predatory bacteria in the termination of a cyanobacterial bloom[J]. Microb Ecol, 2001, 41(2): 97-105.
[10] 姜怡, 唐蜀昆, 王永霞, 等. 海洋放线菌分离方法[J]. 微生物学通报, 2006, 33 (6): 153-155.
[11] 曾湘, 郑天凌. 海洋活性肽研究的回顾与展望[J]. 世界科技研究与发展, 2009, 31(6): 1012-1016.
[12] SAFFERMAN R S, MORRIS M E. Evaluation of natural products for algicidal properties[J]. Appl Environ Microbiol, 1962, 10(4): 289-292.
[13] WHYTE LG, MAULE A, CULLIMORE D R. Method for isolating cyanobacteria-lysing Streptomycetes from soil[J]. Journal of Applied Microbiology, 1985, 58(2): 195-197.
[14] BIABANI M A, BAAKE M, LOVISETTO B, et al. Anthranilamides: new antimicroalgal active substances from a marine Streptomycetes sp.[J]. J Antibiot, 1998, 51(3): 333-340.
[15] SIGEE D C, GLENN R, ANDREWS M J, et al. Biological control of cyanobacteria: principles and possibilities[J]. Hydrobiologia, 1999, 395/396: 161-172.
[16] CHOI H J. Streptomyces neyagawaensis as a control for the hazardous biomass of Microcystis aeruginosa (Cyanobacteria) in eutrophic freshwaters[J]. Biological Control, 2005, 3(2): 335-343.
[17] 王慧玲, 叶新, 赵以军, 等. 一株高效溶藻放线菌的分子生物学鉴定[J]. 环境科学与技术, 2009, 32(6): 17-19.
[18] 崔妍, 李建宏, 汪燕, 等. 高效抑藻放线菌的筛选和活性[J] . 生态学报, 2008, 28(11): 5691-5697.
[19] 许珂, 吴刚, 余文平, 等. 溶藻细菌W5培养条件优化及发酵培养[J]. 环境科学与技术, 2009, 32(2): 12-15.
[20] 肖慈琼, 姜红, 程凯, 等. 溶藻放线菌AN02的筛选及其培养条件的优化[J]. 微生物学杂志, 2007, 27(4): 11-13.
[21] 方开泰. 均匀设计与均匀设计表[M]. 北京: 科学出版社, 1994: 1-62.
[22] 孔贇, 缪礼鸿, 曾驰, 等. 溶藻链霉菌SG-001发酵条件的优化及溶藻活性物质的理化性质[J]. 湖北农业科学, 2009, 6(48): 1361-1364.
[23] 杨丽英, 马国顺. 均匀设计在微生物发酵条件优化试验中的应用[J]. 太原师范学院学报, 2009, 8(3): 23-25.
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