Journal of Tropical Oceanography >
Establishment of in vitro biofilm model and characteristics of biofilm formation of Vibrio harveyi
Received date: 2010-06-29
Revised date: 2011-03-28
Online published: 2011-07-20
Modi?ed microtiter-plate test was used to establish an in vitro biofilm model of Vibrio harveyi TS-628 strain. Effect
of environmental factors on bacterial biofilm formation in vitro was also studied. The results show that V. harveyi TS-628
strains could form biofilm in polystyrene plates, and biofilm formation of this bacterium was significantly affected by different
environmental factors, such as temperature, initial bacterial concentration, pH, NaCl concentration, and so on. The optimum
conditions for biofilm formation of V. h ar v e y i TS-628 strain are at temperature of 30℃, pH=7, NaCl concentration of
3%−6%, and using 36h incubation time. Furthermore, V. harveyi TS-628 strain could form biofilm on the walls coated with
skin mucus, gill mucus, intestinal mucus, liver extract, spleen extract, and muscle extract. The biofilm formation of V. ha r v e y i
on the walls coated with liver extract exhibited a remarkably higher value of D(590)(p<0.01)than that on the walls coated with
other tissue extracts.
LI Hai-ping ,YAN Qing-pi ,XU Xiao-jin ,SU Yong-quan ,QIN Ying-xue . Establishment of in vitro biofilm model and characteristics of biofilm formation of Vibrio harveyi[J]. Journal of Tropical Oceanography, 2011 , 30(3) : 99 -104 . DOI: 10.11978/j.issn.1009-5470.2011.03.099
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