In order to investigate the infected rates of White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV) and Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) among aquatic Litopenaeus vannamei, we tested the samples from western Guangdong Province using Polymerase Chain Reaction (PCR) and Loop-mediated Isothermal Amplication (LAMP). The results show that there were higher infected rates of WSSV and TSV, which suggests these two viruses were the dominant pathogens in this region. These data reveal that the LAMP method was equivalent to the PCR method in specificity and sensitivity, but the LAMP method was more convenient, rapid and low cost than the PCR.
Jiang-Xiao
,
Lin-Chun-Hua-
,
Hu-Chao-Qun-
,
Diao-Zhe
. Epidemiological investigation of three kinds of major virus in Litopenaeus vannamei from western Guangdong Province[J]. Journal of Tropical Oceanography, 2011
, 30(6)
: 97
-103
.
DOI: 10.11978/j.issn.1009-5470.2011.06.097
[1] 宋盛宪. 对虾养殖现状、发展趋势与对策 [J]. 水产科技, 2006 (2): 1-7.
[2] 史成银, 黄倢, 杨冰, 等. 应用 PCR 和 RT-PCR 技术对 4 种对虾病毒的检测 [J]. 海洋水产研究, 2003, 24 (1): 1-5.
[3] 谢芝勋, 谢丽基, 庞耀珊等. 三种对虾病毒多重荧光 PCR 检测方法的建立 [J]. 农业生物技术学报, 2008, 16 (5): 909-910.
[4] 胡超群, 沈琪, 任春华, 等. 快速检测对虾传染性皮下及造血组织坏死病毒试剂盒: 中国, 1410549[P]. 2003-04-16.
[5] 胡超群, 沈琪, 任春华, 等. 一种快速检测对虾桃拉病毒试剂盒: 中国, 1410550[P]. 2003-04-16.
[6] 赵哲, 任春华, 胡超群, 等. 一种用于检测对虾白斑综合症病毒的试剂盒及其检测方法: 中国, 101691615A[P]. 2010-04-07.
[7] 赵哲, 任春华, 胡超群, 等. 一种用于检测对虾传染性皮下及造血组织坏死病毒的试剂盒及其检测方法: 中国, 101643793[P]. 2010-02-10.
[8] 赵哲, 任春华, 胡超群, 等. 一种用于快速检测对虾桃拉病毒的 LAMP 试剂盒及其检测方法: 中国, 101845519A [P]. 2010-09-29.
[9] 宋晓玲, 史成银, 黄倢, 等. 用 DNA 斑点杂交法检测对虾及其饵料和环境生物携带白斑综合症病毒状况的调查 [J]. 中国水产科学, 2001, 8(4): 36-40.
[10] 宣引明, 陆小平, 戴永良, 等. 南美白对虾养殖主要病害分析及对策 [J]. 科学养鱼, 2009 (3): 48-49.
[11] 江世贵, 何建国, 吕玲, 等. 白斑综合症病毒对斑节对虾亲虾的感染及垂直传播的初步研究 [J]. 中山大学学报: 自然科学版, 2000, 39( 增刊 ): 164-171.
[12] LO C F, HO C H, CHEN C H, et al. Detection of tissue tropism of white spot syndrome baculovirus (WSSV) in peneaidbrooders of Penaeus monodon with a special emphasis on reproductive organs[J]. Disease of Aquatic Organisms, 1997, 30: 53-72.
[13] S UN Z F, HU C Q, REN C H, et al. Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated iso thermal amplification[J]. Journal of Virological Methods, 2006, 131 (1): 41-46.
[14] 赵哲, 任春华, 江晓, 等. 荧光定量 PCR 与 LAMP 检测 IHHNV 的特异性和灵敏性比较 [J]. 水生生物学报, 2010, 34(5): 984-989.
[15] KALAGAYAN H, GODIN D, KANNA R, et al. IHHN virus as an etiological factor in runt-deformity syndrome (RDS) of juvenile Penaeus vannamei cultured in Hawaii[J]. Journal of the World Aquaculture Society, 1991, 22 (4): 235-243.
[16] LIGHTNER D V, BELL T A, REDMAN R M, et al. A review of some major diseases of economic significance in peneaidprawns/shrimps of the Americas and Indo-Pacific[R] Manila, Philippines: Asian Fisheries Society, 1992: 57-80.
[17] LIGHTNER D V. A handbook of shrimp pathology and diagnostic procedures of diseases of cultured peneaid shrimp [M]. World Aquaculture Society, 1996: 305.
[18] LIGHTNER D V. The peneaid shrimp viruses IHHNV and TSV: epizootiology, production impacts and role of international trade in their distribution in the Americas[J]. Revue Scientifique et Technique Office International des Epizooties, 1996b, 15: 579-601.
[19] BROWDY C L, HOLLOWAY J D, KING C O, et al. IHHN virus and intensive culture of Penaeus vannamei: effects of stocking density and water exchange rates[J]. Journal of Crustacean Biology, 1993, 13: 87-94.
[20] 庞德彬, 彭劲松, 邢华. 南美白对虾桃拉病毒 (TSV) 防治技术 [J]. 中国水产, 2001, 6: 86-87.
[21] 张文革. 南美白对虾常见病害及其防治技术 [J]. 齐鲁渔业, 2010, 27 (3): 39-40.
[22] 谢芝勋, 庞耀珊, 何竞铭, 等. 应用多重 PCR 检测鉴别对虾白斑综合征病毒和桃拉病毒 [J]. 中国兽医学报, 2005, 25 (1): 13-15.
