Journal of Tropical Oceanography >
Study on the AFLP primer selection and genetic diversities of 4 Snappers
Received date: 2012-04-07
Revised date: 2012-06-05
Online published: 2012-10-24
4 species of snappers (Lutjanus russellii, L.erythropterus, L.argentimaculatus and L.sebae) were analyzed in the study using the AFLP technique with restriction endonuclease of EcoRⅠ/MselⅠmix, so as to develop AFLP Markers and analyze genetic diversity. 50 pairs of AFLP (amplified fragment length polymorphism) primers were used, 24 of which were selected for L. russellii, 20 for L. argentimaculatus, and 22 for both L. erythropterus and L. sebae, the polymorphism sites were 1431, 1370, 1403 and 1349 in turn, their proportion separately was 22.85%, 17.08%, 17.18% and 12.90%. The Nei’s genetic diversity was analyzed in 4 snapper species, the average genetic distance was 0.1035, 0.0719, 0.0805 and 0.0572, respectively. AFLP fingerprinting of 4 snappers with 2 primers (A4, C4) revealed a total number of 140 sites, of which 104 ones were polymorphic. Genetic distance among the 4 snappers ranged from 0.3773 to 0.6650, which was significantly higher than the ones within species. The genetic distances and phylogenic trees both show that L.russellii and L.erythropterus are closest and L.argentimaculatus and L.sebae are furthest.
LIU Li , ZHAO Jie , GUO Yu-song , LIU Chu-wu . Study on the AFLP primer selection and genetic diversities of 4 Snappers[J]. Journal of Tropical Oceanography, 2012 , 31(4) : 112 -116 . DOI: 10.11978/j.issn.1009-5470.2012.04.015
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