Journal of Tropical Oceanography >
Genetic analysis of the marine mussel Perna viridis (Bivalvia: Mytilidae) based on mitochondrial COI sequence and its phylogenetic relationship with other congeneric species
Received date: 2007-11-09
Revised date: 2008-05-04
Online published: 2009-12-12
Supported by
国家自然科学基金项目(30570242);国家“863”计划项目(2006AA10A409)
Partial mitochondrial COI gene sequences from the gonads (male and female) and somatic tissue of mussel Perna viridis were separately amplified by PCR using the universal primers COIL 1490/COIH 2198. A 661 bp nucleotide sequence of COI gene was obtained. It was found that this gene sequence was identical among different tissues of male individual, and low divergence (0.31%) of COI sequence was detected between male and female of the gonad tissues. Only one haplotype was found in both female and male for the sequence. No doubly uniparental inheritance (DUI) was detected. Based on the COI sequences obtained from this study and those of other Perna species from GenBank, NJ tree, MP tree and Bayesian inference (BI) tree were constructed with PAUP and MrBayes respectively, and phylogenetic relationships among them were analyzed accordingly.
WEI Zheng-peng , KONG Xiao-yu , WU Xiang-yun . Genetic analysis of the marine mussel Perna viridis (Bivalvia: Mytilidae) based on mitochondrial COI sequence and its phylogenetic relationship with other congeneric species[J]. Journal of Tropical Oceanography, 2009 , 28(6) : 72 -78 . DOI: 10.11978/j.issn.1009-5470.2009.06.072
[1] 廖顺尧,鲁 成.动物线粒体基因组研究进展 [J].生物化学与生物物理进展.2000; 27(5):508-512.
[2] STEWART, D.T., SAAVEDRA, C., STANWOOD, R.R., et al. Male and female mitochondrial DNA lineages in the blue mussel (Mytilus edulis) species group [J]. Mol. Biol. Evol., 1995, 12: 735–747.
[3] BEAGLEY, C.T., TAYLOR, K.A, WOLSTENHOLME, D.R. Gender associated diverse mitochondrial DNA molecules of the mussel Mytilus californianus[J]. Curr. Genet., 1997, 31: 318–324.
[4] MARCO PASSAMONTI. An unusual case of gender-associated mitochondrial DNA heteroplasmy: the mytilid Musculista senhousia (Mollusca Bivalvia). BMC Evol Biol. 2007: 7(Suppl 2): S7,10.1186/1471-2148-7-S2-S7.
[5]HOEH, W.R., STEWART, D.T., SUTHERLAND, B.W., et al. Cytochrome c oxidase sequence comparisons suggest an unusually high rate of mitochondrial DNA evolution in Mytilus (Mollusca: Bivalvia) [J]. Mol. Biol. Evol., 1996, 13: 418–421.
[6] LUI, H.P., MITTON, J.B., and WU, S.K. Paternal mitochondrial DNA differentiation far exceeds maternal DNA and allozyme differentiation in the freshwater mussel, Anodonta grandis grandis [J]. Evolution, 1996, 50: 952–957.
[7]PASSAMONTI, M., and SCALI, V. Gender-associated mitochondrial DNA heteroplasmy in the venerid clam Tapes philippinarum (Mollusca Bivalvia) [J]. Curr. Genet., 2001, 39: 117–124.
[8] ZOUROS, E., BALL, A.O., SAAVEDRA, C., et al. Mitochondrial DNA inheritance [J]. Nature (London), 1994, 368: 818
[9]SKIBINSKI, D.O.F., GALLAGHER, C., and BEYNON, C.M. Sex-limited mitochondrial DNA transmission in the marine mussel Mytilus edulis [J ]. Genetics, 1994, 138: 801–809.
[10] ZBAWICKA M., SKIBINSKI D.O.F, WENNE R. Doubly uniparental transmission of mitochondrial DNA length variants in the mussel Mytilus trossulus [J]. Mar. Biol., 2003.142: 455-460
[11] 王祯瑞.中国动物志贻贝目 [M], 北京:科学出版社, 1997, 59-62.
[12] WOOD,A.R., APTE, S., MACAVOY, E.S. et al. A molecular phylogeny of the marine mussel genus Perna (Bivalvia: Mytilidae) based on nuclear (ITS1&2) and mitochondrial (COI) DNA sequences [J]. Mol Phylogenet Evol, United States 2007, 44(2): 685-698
[13] BLAIR, D., WAYCOTT, M., BYRNE, L. et al. Molecular Discrimination of Perna (Mollusca: Bivalvia) Species Using the Polymerase Chain Reaction and Species-Specific Mitochondrial Primers [J]. Mar. Biotechnol.2006, 8 (4), 380-385.
[14] APTE, S and GARDNER J.P.A, Gene order for a segment of the mitochondrial genome of the greenshell mussel, Perna canaliculus (Mytilidae: Bivalvia) [J]. J. Moll. Stud., 2002, 68: 283-286.
[15] RAWSON, P.D., HILBISH, T.J. Evolutionary relationships among the male and female mitochondrial DNA lineages in the Mytilus edulis species complex [J]. Mol. Biol. Evol.1995, 12, 893–901.
[16] BUCKLIN A , GUARNIERI M , HILL R S , et al. Taxonomic and systematic assessment of planktoic copepods using mitochondrial COI sequence variation and competitive species-specific PCR[J] . Hydrobiologia, 1999, 401:239-254.
[17]THOMPSON J D, GIBSON T J, PLEWNIAK F, et al. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J]. Nucleic Acids Res., 1997, 25: 4876–4882.
[18] TAMURA K, DUDLEY J, NEI M, et al. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599.
[19] SWOFFORD,D.L. PAUP*Phylogenetic Analysis Using Parsimony (and Other Methods). Version 4. Sinauer, Sunderland, Massachusetts, 2000
[20] RONQUIST,F.and HUELSENBECK J.P.. MRBAYES 3: Bayesian phylogenetic inference under mixed models [J]. Bioinformatics, 2003, 19:1572-1574
[21]HASTINGS,W.K. Monte Carlo sampling methods using Markov chains and their applications[J]. Biometrika, 1970, 57:97-109.
[22] AVISE, J. C. Phylogeography. The history and formation of species. [M]. Cambridge, MA: Harvard University Press. 2000
[23] SATTA Y., and TAKAHATA N. Evolution of Drosophila mitochondrial DNA and the history of the melanogaster subgroup [J]. Proc. Natl. Acad. Sci. USA, 1990, 87: 9558-9562.
[24] SPICER G B. Phylogenetic utility of the mitochondrial cytochrome oxidase gene: molecular evolution of the Drosophila buzzatii species complex [J]. Mol Evol, 1995, 41: 749-759.
[25] CROZIER R H, CROZIER Y C, MACKINLAY A G. The COI and COII region of honeybee mitochondrial DNA: evidence for variation in insect mitochondrial evolutionary rates [J]. Mol. Bio. Evol. 1989, 6(4): 399-411
[26] MIZI A, ZOUROS E, MOSCHONAS N, et al. The complete maternal and paternal mitochondrial genomes of the Mediterranean mussel Mytilus galloprovincialis: implications for the doubly uniparental inheritance mode of mtDNA [J]. Mol Biol Evol 2005, 22, 952–967
[27] ZHENG XIAO DONG, WANG RU CAI, WANG XIAO FFENG,et al.Genetic variation in population of the common Chinese cuttlefish Sepiella maindroni (Mollusca Cephalopoda) using allozymes and mitochondrial DNA sequence analysis [J].J. Shellfish Res.,2001,20 (3): 1159-1165.
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